01875nas a2200217   4500008004100000022001400041245013000055210006900185260009000254300001000344490000700354520103800361653002201399653001501421653002301436653001601459653001601475100002001491700001701511856012901528       2013                        eng d  a1024-242200aBiotransformation of vinblastine to vincristine by the endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aBiotransformation of vinblastine to vincristine by the endophyti  aTELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLANDbINFORMA HEALTHCAREcAPR  a89-930 v313 a<p>A fungal transformation method for the production of the less abundant but more valuable anticancer drug vincristine using the more abundant anticancer drug vinblastine by an endophytic fungus Fusarium oxysporum isolated from the plant Catharanthus roseus is described. Vinblastine dissolved in sterile water when incubated with the fungal mycelial mass was transformed into vincristine at room temperature. The biotransformation process was monitored by periodic sampling of aliquots that were extracted with ethyl acetate. The crude transformed compounds were analysed using TLC where the Rf value of the spot obtained on the 8th day was found to be similar to that of standard vincristine (Rf 0.74). Purification by HPLC using a C 18 symmetry column showed a peak with RT (34.9 min) similar to that of standard vincristine. Further characterization with the help of ESI-MS showed a peak at m/z 825 attributed to the (M + H)(+) ion. This indicated that the fungus Fusarium oxysporum had converted vinblastine to vincristine.</p>10aBiotransformation10aendophytes10afusarium oxysporum10aVinblastine10avincristine1 aKumar, Ashutosh1 aAhmad, Absar  uhttps://library.ncl.res.in/content/biotransformation-vinblastine-vincristine-endophytic-fungus-fusarium-oxysporum-isolated-0