02296nas a2200241   4500008004100000022001400041245011800055210006900173260006700242300001200309490000700321520147000328653001001798653001801808653001701826653001101843653000901854653000801863100001801871700002101889700001701910856012701927       2012                        eng d  a0885-451300aPhytosynthesis of intracellular and extracellular gold nanoparticles by living peanut plant (Arachis hypogaea L.)0 aPhytosynthesis of intracellular and extracellular gold nanoparti  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcNOV  a471-4780 v593 a<p>Inorganic nanomaterials of different chemical compositions are conventionally synthesized under harsh environments such as extremes of temperature, pressure, and pH. Moreover, these methods are eco-unfriendly and cumbersome, yield bigger particles, and agglomerate because of not being capped by capping agents. In contrast, biological synthesis of inorganic nanomaterials occurs under ambient conditions, namely room temperature, atmospheric pressure, and physiological pH. These methods are reliable, eco-friendly, and cheap. In this paper, we report for the first time the extracellular and intracellular synthesis of gold nanoparticles (GNPs) using living peanut seedlings. The formed GNPs were highly stable in solution and inside the plant tissue. Transmission electron microscopy revealed that extracellular GNPs distributions were in the form of monodispersed nanoparticles. The nanoparticles ranged from 4 to 6 nm in size. The intercellular nanoparticles were of oval shape and size ranged from 5 to 50 nm. Both extracellular and intracellular nanoparticles were further characterized by standard techniques. The formed GNPs inside the plant tissue were estimated by inductively coupled plasma spectrometry. This opens up an exciting possibility of a plant-based nanoparticle synthesis strategy, wherein the nanoparticles may be entrapped in the biomass in the form of a film or produced in the solution, both of which have interesting applications.</p>10acells10aextracellular10aliving plant10aPeanut10aroot10aTEM1 aRaju, Dugyala1 aMehta, Urmil, J.1 aAhmad, Absar  uhttps://library.ncl.res.in/content/phytosynthesis-intracellular-and-extracellular-gold-nanoparticles-living-peanut-plant-002302nas a2200241   4500008004100000022001400041245008900055210006900144260011100213300001200324490000600336520145700342653001701799653001101816653002301827653001501850653001101865653000801876100001301884700002101897700001701918856012501935       2013                        eng d  a1573-413700aExtra- and intracellular gold nanoparticles synthesis using live peanut callus cells0 aExtra and intracellular gold nanoparticles synthesis using live   aEXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATESbBENTHAM SCIENCE PUBL LTDcFEB  a107-1120 v93 a<p>An important challenge of considerable topical significance in nanotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials in large quantities with variable sizes, shapes and chemical compositions. Green synthesis routes for the production of inorganic metal nanoparticles using whole cell of microorganisms and plant extracts are gaining tremendous popularity as these are non-toxic, cheap and occur at ambient conditions. The present work emphasizes on gold nanoparticles synthesis protocol using live plant callus cells. Peanut callus cells when incubated with HAuCl4 solution in ambient conditions reduced the precursor and lead to formation of well dispersed, water soluble extracellular and intracellular gold nanoparticles within 24 hours. The biosynthesis of gold nanoparticles was monitored by UV-visible spectroscopy (UV-Vis) and further characterized by X-ray diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Selected Area Electron Diffraction (SAED). The particle size distribution shows that the average particle size is 50 nm for extra-and 31 nm for intracellular gold nanoparticles. The nanoparticles may be stabilized by proteins secreted by callus cells. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, plant cell-based method for the synthesis of nanoparticles over a wide range of chemical compositions.</p>10aBiosynthesis10acallus10agold nanoparticles10alive cells10aPeanut10aTEM1 aRaju, D.1 aMehta, Urmil, J.1 aAhmad, Absar  uhttps://library.ncl.res.in/content/extra-and-intracellular-gold-nanoparticles-synthesis-using-live-peanut-callus-cells-001839nas a2200229   4500008004100000022001400041245008300055210006900138260009200207300001400299490000700313520105400320653002301374653001101397653001301408653001501421653001001436100001301446700002101459700001701480856011201497       2015                        eng d  a1533-488000aSimple recovery of intracellular gold nanoparticles from peanut seedling roots0 aSimple recovery of intracellular gold nanoparticles from peanut   a26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USAbAMER SCIENTIFIC PUBLISHERScFEB  a1575-15810 v153 a<p>Fabrication of inorganic nanomaterials via a biological route witnesses the formation either extracellularly, intracellulary or both. Whereas extracellular formation of these nanomaterials is cherished owing to their easy and economical extraction and purification processes; the intracellular formation of nanomaterials, due to the lack of a proper recovery protocol has always been dreaded, as the extraction processes used so far were tedious, costly, time consuming and often resulting in very low recovery. The aim of the present study was to overcome the problems related with the extraction and recovery of intracellularly synthesized inorganic nanoparticles, and to devise a method to increasing the output, the shape, size, composition and dispersal of nanoparticles is not altered. Water proved to be much better system as it provided well dispersed, stable gold nanoparticles and higher recovery. This is the first report, where intracellular nanoparticles have been recovered using a very cost-effective and eco-friendly approach.</p>10agold nanoparticles10aPeanut10aRecovery10aSonication10aWater1 aRaju, D.1 aMehta, Urmil, J.1 aAhmad, Absar  uhttps://library.ncl.res.in/content/simple-recovery-intracellular-gold-nanoparticles-peanut-seedling-roots-0