02302nas a2200241   4500008004100000022001400041245008900055210006900144260011100213300001200324490000600336520145700342653001701799653001101816653002301827653001501850653001101865653000801876100001301884700002101897700001701918856012501935       2013                        eng d  a1573-413700aExtra- and intracellular gold nanoparticles synthesis using live peanut callus cells0 aExtra and intracellular gold nanoparticles synthesis using live   aEXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATESbBENTHAM SCIENCE PUBL LTDcFEB  a107-1120 v93 a<p>An important challenge of considerable topical significance in nanotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials in large quantities with variable sizes, shapes and chemical compositions. Green synthesis routes for the production of inorganic metal nanoparticles using whole cell of microorganisms and plant extracts are gaining tremendous popularity as these are non-toxic, cheap and occur at ambient conditions. The present work emphasizes on gold nanoparticles synthesis protocol using live plant callus cells. Peanut callus cells when incubated with HAuCl4 solution in ambient conditions reduced the precursor and lead to formation of well dispersed, water soluble extracellular and intracellular gold nanoparticles within 24 hours. The biosynthesis of gold nanoparticles was monitored by UV-visible spectroscopy (UV-Vis) and further characterized by X-ray diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Selected Area Electron Diffraction (SAED). The particle size distribution shows that the average particle size is 50 nm for extra-and 31 nm for intracellular gold nanoparticles. The nanoparticles may be stabilized by proteins secreted by callus cells. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, plant cell-based method for the synthesis of nanoparticles over a wide range of chemical compositions.</p>10aBiosynthesis10acallus10agold nanoparticles10alive cells10aPeanut10aTEM1 aRaju, D.1 aMehta, Urmil, J.1 aAhmad, Absar  uhttps://library.ncl.res.in/content/extra-and-intracellular-gold-nanoparticles-synthesis-using-live-peanut-callus-cells-0