03345nas a2200253   4500008004100000022001400041245013800055210006900193260000800262520238100270653003102651653002102682653003102703653002302734653001902757653003702776100002702813700002302840700002102863700001902884700002802903700003002931856013002961       2024                        eng d  a1867-130600aDietary supplementation of lactococcus lactis subsp. lactis BIONCL17752 on growth performance, and gut microbiota of broiler chickens0 aDietary supplementation of lactococcus lactis subsp lactis BIONC  cJUN3 a<p>
	The rapid rise of antimicrobial resistance (AMR) is a global concern, being triggered by the overuse or misuse of antibiotics in poultry farming sector. We evaluated Lactococcus lactis subsp. lactis BIONCL17752 strain, and characterized its probiotic potential to endure hostile gastrointestinal conditions. Genome sequencing analysis revealed probiotics traits, and gene clusters involved in bacteriocins, lactococcin A, and sactipeptides production. The absence of genes for antibiotic resistance, virulence, and biogenic amine production indicates the potential of probiotic strain. The BIONCL17752 strain was explored for antibiotic-free feed supplement for growth promotor in broiler chicken. The feed supplemented with 4 x 109 CFU/kg of probiotic strain, in combination with various concentrations of fructooligosaccharides (FOS) 1.0, 2.5, and 5.0 kg/tonne in starter, grower, and finisher diets, respectively. A significant improvement of body weight 152 to 171 g/bird (p &lt; 0.05), and a low feed conversion ratio (FCR) of 1.62, was achieved without using synthetic antibiotics for growth promotion. The results of biochemical, hematological, and histological examinations showed normal features, indicating that the treatment had no harmful effects on the bird's health. Reduced levels of cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) in serum are an indication of the health benefits for the treated birds. Microbial community analysis of fecal samples of poultry birds exhibited a higher abundance of Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, and Fusobacteria. Probiotic treatment resulted in reduced Firmicutes and increased Bacteroidetes (F/B ratio) in the broiler's gut which highlights the benefits of probiotic dietary supplements. Importantly, the probiotic-fed group exhibited a high abundance of carbohydrate-active enzymes (CAZyme) such as glycoside hydrolases (GH), glycoside transferases (GT), and carbohydrate-binding module (CBM) hydrolases which are essential for the degradation of complex sugar molecules. The probiotic potential of the BIONCL17752 strain contributes to broilers' health by positively affecting intestinal microbiota, achieving optimal growth, and lowering mortality, demonstrating the economic benefits of probiotic treatment in organic poultry farming.</p>
10aAntibiotic growth promotor10aBroiler chickens10aFunctional genome analysis10aGrowth performance10aGut microbiota10aLactococcus lactis subsp. lactis1 aNavale, Vishwambar, D.1 aYadav, Rakeshkumar1 aKhilari, Ajinkya1 aDharne, Mahesh1 aShanmugam, Dhanasekaran1 aVamkudoth, Koteswara, Rao  uhttps://library.ncl.res.in/content/dietary-supplementation-lactococcus-lactis-subsp-lactis-bioncl17752-growth-performance-and02305nas a2200313   4500008004100000245009800041210006900139260000800208300001200216490000700228520125200235653002901487653002301516653002701539653003101566653002301597100001901620700002101639700002701660700001901687700002501706700002401731700002101755700002001776700002701796700001801823700002801841856012201869       2025                        eng d00aDetection and variant characterization of lumpy skin disease virus from dairy cattle in India0 aDetection and variant characterization of lumpy skin disease vir  cDEC  aveaf0900 v113 a<p>
	The spread of a severe and often fatal form of lumpy skin disease (LSD) in cattle and water buffaloes has caused widespread mortality and morbidity of these animals in India. To track and understand the genetic changes occurring in the virus and to enable routine surveillance of the virus, multiplexed polymerase chain reaction (PCR) and sequencing methods were developed and validated in this study. Multiplexed nested PCR for LSD virus (LSDV) detection was optimized using skin lesion swabs and nasal samples collected from symptomatic and asymptomatic animals. For genotyping, overlapping PCRs to amplify the entire LSDV genome were developed and tested on field samples collected from the Maharashtra and Odisha states of India. Analysis of LSDV genomes from 41 field samples collected in 2022 and 2023 revealed the presence of highly conserved novel mutations. Phylogenetic analysis shows that a distinct genotype of LSDV has spread across India, which warrants genomic surveillance of the virus in the coming years to track the evolution and transmission of the virus. The non-invasive sample collection, detection, and genotyping methods described in this study can facilitate large-scale surveillance of LSDV in dairy animals.</p>
10agenotyping by sequencing10alumpy skin disease10amultiplexed nested PCR10aOxford Nanopore technology10avirus surveillance1 aBajpai, Manali1 aKhilari, Ajinkya1 aLikhitkar, Bhagyashree1 aMusale, Pankaj1 aJadhav, Santoshkumar1 aDhanikachalam, Velu1 aKakramkar, Payal1 aBhave, Kaustubh1 aSwaminathan, Marimuthu1 aJoshi, Sachin1 aShanmugam, Dhanasekaran  uhttps://library.ncl.res.in/content/detection-and-variant-characterization-lumpy-skin-disease-virus-dairy-cattle-india