01983nas a2200253   4500008004100000022001400041245008300055210006900138260005700207300001200264490000700276520111600283653002201399653003001421653001401451653001901465653002401484653001601508100002701524700002101551700001801572700002501590856011401615       2015                        eng d  a1618-954X00aEco-friendly enzymatic dehairing of skins and hides by C-brefeldianus protease0 aEcofriendly enzymatic dehairing of skins and hides by Cbrefeldia  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcFEB  a393-4050 v173 a<p>Alkaline protease from Conidiobolus brefeldianus was efficient in unhairing various types of skins and hides. The crude protease preparation was active toward keratin-azure, elastin-orcin, azocasein, and azocoll, but did not show true collagenase activity. In addition, the crude enzyme exhibited other enzyme activities such as chondroitinase, laminarase, and chitinase. Complete hair removal of skin/hide by the protease achieved in 16-18 h. The dehaired pelt showed smooth and white appearance due to hair removal along with epidermal layer. In addition, the grain was clean and without damage in enzymatically dehaired pelts. The microscopic observation of the cross-section of dehaired goat skin and cow hide showed absence of epidermis, hair shaft with empty follicles. Enzymatic dehairing resulted in complete and uniform fiber opening in the dermis and corium region. Physical properties viz. tensile strength, elongation, and tear strength of dyed crust of enzymatically and conventionally dehaired pelts were comparable. Results were also validated on large scale with goat skins and cow hides.</p>10aAlkaline Protease10aConidiobolus brefeldianus10aCow hides10aDehaired pelts10aEnzymatic dehairing10aSheep skins1 aKhandelwal, Harish, B.1 aMore, Snehal, V.1 aKalal, K., M.1 aLaxman, Ryali, Seeta  uhttps://library.ncl.res.in/content/eco-friendly-enzymatic-dehairing-skins-and-hides-c-brefeldianus-protease-001731nas a2200229   4500008004100000022001400041245013800055210006900193260000800262300000900270490000700279520093700286653002201223653001401245653001401259653000901273653001601282100002101298700002001319700003101339856013101370       2017                        eng d  a1544-047800aSilk degumming and utilization of silk sericin by hydrolysis using alkaline protease from beauveria Sp. (MTCC 5184): a green approach0 aSilk degumming and utilization of silk sericin by hydrolysis usi  cJUL  a1-110 v153 aConventionally, degumming is carried out at 90°C--110°C temperature by boiling the raw silk with Marseilles soap and sodium bicarbonate which eventually requires a lot of water and energy. In this study, degumming of Chinese bivoltine raw silk fibres with alkaline protease produced by Beauveria sp. (MTCC 5184) is studied. Complete degumming was obtained in 45 min with 75 units of enzyme per gram of silk. Degumming was found to be optimal at 50°C and pH 9.0. Scanning electron micrographs showed that the sericin deposits were removed and the obtained fibres were clean, separated, had smooth feel with shine as compared to untreated fibres. Sericin isolated from silk cocoon (by-product which goes waste) was hydrolyzed with the same alkaline protease obtained from Beauveria sp. to get small molecular weight peptides. These peptides can be utilized further for cosmetic, pharmaceutical, and various industrial applications.10aAlkaline Protease10abivoltine10adegumming10aSilk10aweight loss1 aMore, Snehal, V.1 aChavan, Sakalya1 aPrabhune, Asmita, Ashutosh  uhttps://library.ncl.res.in/content/silk-degumming-and-utilization-silk-sericin-hydrolysis-using-alkaline-protease-beauveria-sp