01253nas a2200181 4500008004100000245012600041210006900167260000800236300001400244490000700258520056900265100002500834700001900859700002000878700002300898700002500921856012500946 2019 eng d00aIsolation and molecular characterization of thermophilic coprophilous fungus malbranchea cinnamomea GSMBKU from goat dung0 aIsolation and molecular characterization of thermophilic coproph cDEC a2227-22330 v133 a

Thermophilic fungi, found in the varied habitats are valuable sources for potential enzymes and other biochemical metabolites throwing challenges to the mycologists. A study of such fungi will not only enables the biochemists, microbiologists, and the biotechnologists to understand the basic molecular process but also search for novel genes, gene products and reactions. In this study, Malbranchea cinnamomea was isolated from goat dung and characterized based on its morphological and physiological characters, and later confirmed molecularly.

1 aAjmera, Shanthipriya1 aRao, Koteswara1 aRanipadmini, V.1 aMerugu, Ramchander1 aGirisham, Sivadevuni uhttps://library.ncl.res.in/content/isolation-and-molecular-characterization-thermophilic-coprophilous-fungus-malbranchea04532nas a2200169 4500008004100000245010000041210006900141260000800210300001400218490000600232520390100238100002304139700003004162700002504192700002004217856012504237 2021 eng d00aAspergillus derived mycotoxins in food and the environment: prevalence, detection, and toxicity0 aAspergillus derived mycotoxins in food and the environment preva cJAN a1008-10300 v83 a

Aspergillus species are the paramount ubiquitous fungi that contaminate various food substrates and produce biochemicals known as mycotoxins. Aflatoxins (AFTs), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), aflatrem (AT), secalonic acids (SA), cyclopiazonic acid (CPA), terrein (TR), sterigmatocystin (ST) and gliotoxin (GT), and other toxins produced by species of Aspergillus plays a major role in food and human health. Mycotoxins exhibited wide range of toxicity to the humans and animal models even at nanomolar (nM) concentration. Consumption of detrimental mycotoxins adulterated foodstuffs affects human and animal health even trace amounts. Bioaerosols consisting of spores and hyphal fragments are active elicitors of bronchial irritation and allergy, and challenging to the public health. Aspergillus is the furthermost predominant environmental contaminant unswervingly defile lives with a 40–90 % mortality risk in patients with conceded immunity. Genomics, proteomics, transcriptomics, and metabolomics approaches useful for mycotoxins’ detection which are expensive. Antibody based detection of toxins chemotypes may result in cross-reactivity and uncertainty. Aptamers (APT) are single stranded DNA (ssDNA/RNA), are specifically binds to the target molecules can be generated by systematic evolution of ligands through exponential enrichment (SELEX). APT are fast, sensitive, simple, in-expensive, and field-deployable rapid point of care (POC) detection of toxins, and a better alternative to antibodies.

1 aNavale, Vishwambar1 aVamkudoth, Koteswara, Rao1 aAjmera, Shanthipriya1 aDhuri, Vaibhavi uhttps://library.ncl.res.in/content/aspergillus-derived-mycotoxins-food-and-environment-prevalence-detection-and-toxicity03136nas a2200181 4500008004100000245009600041210006900137260000800206300001400214490000700228520248000235100001902715700002202734700001802756700003002774700002502804856012502829 2022 eng d00aPectinase production by aspergillus niger and its applications in fruit juice clarification0 aPectinase production by aspergillus niger and its applications i cNOV a2724-27370 v163 a

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions that degrade pectic substances. In the present study, we have isolated the Aspergillus niger strain from soil samples, in India and evaluated pectinase production. The highest pectinase producing A. niger strain was further evaluated and optimized with various agricultural wastes. Plackett-Burman design (PBD) and Central composite design ‘(CCD)’ were used to determine the best parameters for maximum pectinase production. Pectinase activity was increased to 99.21 U/ml after optimizing the production medium using PBD and CCD statistical analysis. A positive correlation of pectinase activity between predicted (112.65 U/ml) and experimental (99.21 U/ml with SD=0.005) optimum was observed. Maximum pectinase was produced by A. niger under submerged fermentation, utilizing orange peel, which is a cost-effective, adaptable, and environmentally friendly approach. The partially purified pectinase showed significant application for apple juice clarification and showed the ability to degrade pectin and therefore the colour change was observed in apple juice within 120 min. Maximum pectinase was produced by A. niger using agricultural waste orange peel under submerged fermentation which is an economical, versatile and eco-friendly process and pectinase showed a significant application for apple juice clarification.

1 aWagh, Vrushali1 aPatel, Hirvitaben1 aPatel, Nafisa1 aVamkudoth, Koteswara, Rao1 aAjmera, Shanthipriya uhttps://library.ncl.res.in/content/pectinase-production-aspergillus-niger-and-its-applications-fruit-juice-clarification