02292nas a2200217   4500008004100000022001400041245014700055210006900202260000900271300001200280490000800292520149700300653001501797653001801812653002701830100002601857700002001883700002501903700002001928856012601948       2019                        eng d  a0141-813000aPotent chitin-hydrolyzing enzyme from Myrothecium verrucaria affects growth and development of Helicoverpa armigera and plant fungal pathogens0 aPotent chitinhydrolyzing enzyme from Myrothecium verrucaria affe  cDEC   a517-5280 v1413 a<p>Chitin, a crucial structural and functional component of insects and fungi, serves as a target for pest management by utilizing novel chitinases. Here, we report the biocontrol potential of recombinant Myrothecium verrucaria endochitinase (rMvEChi) against insect pest and fungal pathogens. A complete ORF of MvEChi (1185 bp) was cloned and heterologously expressed in Escherichia coli. Structure based sequence alignment of MvEChi revealed the presence of conserved domains SXGG and DXXDXDXE specific for GH-18 family, involved in substrate binding and catalysis, respectively. rMvEChi (46.6 kDa) showed optimum pH and temperature as 7.0 and 30 degrees C, respectively. Furthermore, rMvEChi remained stable within the pH range of 6.0 to 8.0 and up to 40 degrees C. rMvEChi exhibited k(cat)/K-m values of 129.83 x 10(3) [(g/L)(-1) s(-1)] towards 4MU chitotrioside. Hydrolysis of chitooligosaccharides with various degrees of polymerization (DP) using rMvEChi indicated the release of DP2 as main end product with order of reaction as DP6 &gt; DP5 &gt; DP4 &gt; DP3. Bioassay of rMvEChi against Helicoverpa armigera displayed potent anti-feedant activity and induced mortality. In vitro antifungal activity against plant pathogenic fungi (Ustilago maydis and Bipolaris sorokiniana) exhibited significant inhibition of mycelium growth. These results suggest that MvEChi has significant potential in enzyme-based pest and pathogen management. (C) 2019 Elsevier B.V. All rights reserved.</p>
10aBiocontrol10aendochitinase10aMyrothecium verrucaria1 aVidhate, Ravindra, P.1 aBhide, Amey, J.1 aGaikwad, Sushama, M.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/potent-chitin-hydrolyzing-enzyme-myrothecium-verrucaria-affects-growth-and-development01935nas a2200253   4500008004100000022001400041245008900055210006900144260000800213300001000221490000700231520111400238653001501352653001501367653002101382653002701403653002301430653001701453100002601470700002301496700002401519700002001543856011801563       2023                        eng d  a0095-362800aGenomic determinants of entomopathogenic fungi and their involvement in pathogenesis0 aGenomic determinants of entomopathogenic fungi and their involve  cJAN  a49-600 v853 a<p>
	Entomopathogenic fungi offer an effective and eco-friendly alternative to curb insect populations in biocontrol strategy. The evolutionary history of selected entomopathogenic fungi indicates their ancestral relationship with plant endophytes. During this host shifting, entomopathogenic fungi must have acquired multiple mechanisms, including a combination of various biomolecules that make them distinguishable from other fungi. In this review, we focus on understanding various biochemical and molecular mechanisms involved in entomopathogenesis. In particular, we attempt to explain the indispensable role of enlarged gene families of various virulent factors, viz. chitinases, proteases, lipases, specialized metabolites, and cytochrome P450, in entomopathogenesis. Our analysis suggests that entomopathogenic fungi recruit a different set of gene products during the progression of pathogenesis. Knowledge of these bio-molecular interactions between fungi and insect hosts will allow researchers to execute pointed efforts towards the development of improved entomopathogenic fungal strains.</p>
10aBiocontrol10aChitinases10aEndophytic fungi10aEntomopathogenic fungi10aHydrolytic enzymes10aInsect pests1 aVidhate, Ravindra, P.1 aDawkar, Vishal, V.1 aPunekar, Sachin, A.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/genomic-determinants-entomopathogenic-fungi-and-their-involvement-pathogenesis