02115nas a2200289   4500008004100000022001400041245010000055210006900155260000800224300001000232490000800242520122000250653001201470653001201482653001901494653001701513653002201530653001201552100002301564700002201587700001801609700001701627700002101644700002201665700001501687856012301702       2022                        eng d  a0960-308500aProcess development for crystalline xylitol production from corncob biomass by Pichia caribbica0 aProcess development for crystalline xylitol production from corn  cMAY  a45-560 v1333 a<p>
	Xylitol, a commodity chemical, is widely used in nutraceutical and pharmaceutical formulations. Microbial xylitol production is a promising alternative to mitigate current industrial practice issues and offers an environment-friendly sustainable conversion route. This study demonstrates a bioprocess for xylitol production from corncob using a mesophilic yeast Pichia caribbica. Corncob was hydrolyzed by dilute acid and steam explosion to recover fermentable xylose and used as the feed for xylitol production. Activated carbon treatment (3% w/v) completely removed the biomass-derived inhibitors furfural and hydroxymethyl furfural from the liquid hydrolysate. The fermenting yeast Pichia caribbica produced 124.1 +/- 0.45 g/L xylitol from the detoxified and concentrated corncob hydrolysate with a high yield of 0.80 +/- 0.02 g/g. The crystallized xylitol with 96.5% purity demonstrated no harmful effects on the cell line used as a control for the in-vitro toxicity studies. This proof of concept can be applied to help scale up for bio-refinery-based large-scale production of xylitol from corncob biomass.(c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.</p>
10aBiomass10aCorncob10aDetoxification10aFermentation10aToxicity analysis10axylitol1 aDasgupta, Diptarka1 aSidana, Arushdeep1 aSarkar, Bipul1 aMore, Snehal1 aGhosh, Debashish1 aBhaskar, Thallada1 aRay, Anjan  uhttps://library.ncl.res.in/content/process-development-crystalline-xylitol-production-corncob-biomass-pichia-caribbica01602nas a2200277   4500008004100000022001400041245011400055210006900169260000800238300001100246490000700257520070400264653002300968653001900991653001701010653001701027653001201044100001801056700002801074700002101102700001801123700001601141700001701157700002301174856012701197       2022                        eng d  a2213-292900aProcess development for detoxification of corncob hydrolysate using activated charcoal for xylitol production0 aProcess development for detoxification of corncob hydrolysate us  cFEB  a1070970 v103 aThe work describes process development for detoxification of corncob hydrolysate using activated carbon for microbial xylitol production. Activated carbon was used as an adsorbent to completely remove the major fermentation inhibitors obtained during dilute acid and steam explosion of biomass. A xylitol yield of 0.78 g/g was achieved from the detoxified hydrolysate in a 5 L fermenter by Pichia caribbica. The spent carbon was effectively regenerated and used for three consecutive cycles of operation. The successive regeneration and reuse of carbon could reduce the operational cost by similar to 38% and be used as a reference dataset for process scale-up and cost-effective xylitol production.10aActivated charcoal10aDetoxification10aFermentation10aRegeneration10axylitol1 aAhuja, Vishal1 aKshirsagar, Siddheshwar1 aGhosh, Prasenjit1 aSarkar, Bipul1 aSutar, Ajit1 aMore, Snehal1 aDasgupta, Diptarka  uhttps://library.ncl.res.in/content/process-development-detoxification-corncob-hydrolysate-using-activated-charcoal-xylitol