02064nas a2200265   4500008004100000022001400041245011200055210006900167260000800236300001400244490000700258520120900265653000601474653001701480653001701497653002101514653001301535653001301548653002701561100001801588700002001606700001901626700002501645856012801670       2021                        eng d  a0268-257500aBioglycerol (C3) upgrading to 2,3-butanediol (C4) by cell-free extracts of Enterobacter aerogenes NCIM 26950 aBioglycerol C3 upgrading to 23butanediol C4 by cellfree extracts  cMAY  a1316-13250 v963 a<p>BACKGROUND Production of biobased chemicals from renewable resources is a green starring approach that serves as a substitute to petroleum derivatives. Bioglycerol, with its growing production as a co-product of biodiesel, is an attractive low-cost feedstock for the synthesis of platform chemicals by microbial fermentation. 2,3-butanediol (2,3-BDO) is amongst the top biorefinery platform chemicals that can be produced by glycerol fermentation. RESULTS The `Circular Economy' concept is demonstrated by converting the by-product bioglycerol using a cell-free extract of Enterobacter aerogenes NCIM 2695 (National Collection of Industrial Microorganisms, NCIM), yielding 22 g L-1 2,3-BDO, in 96 h, 98% atom economy and 0.4 g/g E factor. The cell-free bioglycerol conversion to 2,3-BDO was proved using a modified procedure for determining glycerol dehydrogenase enzyme assay by protein analysis and it was also shown to be cell-bounded. CONCLUSION Our study offers an effective utilization of the leftover material (i.e. cell-free extract) that biocatalysed C3 to C4 diol, which adds value to the overall economics of the process using only crude glycerol (C3) itself as a fermentative medium.</p>
10a210a3-Butanediol10aatom economy10acircular economy10aE factor10aGlycerol10aglycerol dehydrogenase1 aParate, Roopa1 aBorgave, Mrunal1 aDharne, Mahesh1 aRode, Chandrashekhar  uhttps://library.ncl.res.in/content/bioglycerol-c3-upgrading-23-butanediol-c4-cell-free-extracts-enterobacter-aerogenes-ncim