02668nas a2200277   4500008004100000022001400041245008000055210006900135260007400204300001200278490000800290520174300298653001502041653002402056653003302080653001302113100002402126700002502150700002602175700001402201700002002215700001702235700001602252700001602268856010602284       2014                        eng d  a0003-607200aRoseovarius azorensis sp nov., isolated from seawater at espalamaca, azores0 aRoseovarius azorensis sp nov isolated from seawater at espalamac  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR  a571-5780 v1053 a<p>A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084(T), was isolated from a surface seawater sample collected at Espalamaca (38A degrees 33'N; 28A degrees 39'W), Azores. Growth was found to occur from 15 to 40 A degrees C (optimum 30 A degrees C), at pH 7.0-9.0 (optimum pH 7.0) and with 25-100 % seawater or 0.5-7.0 % NaCl in the presence of Mg2+ and Ca2+; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084(T) did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084(T) belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172(T), Roseovarius mucosus DFL-24(T) and Roseovarius lutimaris 112(T) with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed &lt; 95 % similarity. The polar lipids of strain SSW084(T) were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C-18:1 omega 7c (52.5 %) and C-16:0 (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084(T) is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084(T) (=KCTC 32421(T) = MTCC 11812(T)).</p>10aEspalamaca10aPolyphasic taxonomy10aRoseovarius azorensis sp nov10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana  uhttps://library.ncl.res.in/content/roseovarius-azorensis-sp-nov-isolated-seawater-espalamaca-azores-002246nas a2200241   4500008004100000022001400041245008500055210006900140260011800209300001200327490000700339520136700346100002401713700002501737700002001762700002601782700001401808700002001822700001701842700001601859700001601875856011301891       2015                        eng d  a1466-502600aVitellibacter nionensis sp nov., isolated from a shallow water hydrothermal vent0 aVitellibacter nionensis sp nov isolated from a shallow water hyd  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcFEB  a692-6970 v653 a<p>A novel, Gram-stain-negative, non-motile, rod-shaped yellow bacterium, designated VBW088(T) was isolated from a shallow water hydrothermal vent in Espalamaca in the Azores. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain VBW088(T) clustered with three type strains of species of the genus Vitellibacter and exhibited a sequence similarity of 97.3% with Vitellibacter soesokkakensis RSSK-12(T). However, strain VBW088(T) and V. soesokkakensis RSSK-12(T) exhibited low DNA-DNA relatedness (12.7 +/- 3.5 %). Strain VBW088(T) was positive for catalase and oxidase. Growth occurred at 10-37 degrees C, with the optimum at 80 degrees C, and at pH 6.0-8.0 (optimum pH 6.0) and in up to 5% (w/v) NaCl with optimum growth at 1-2% (w/v) NaCl. The major fatty acids (&gt;10%) were iso-C-15:0 (33.5%) and iso-C-17:0 3-OH (32.0%). The polar lipids detected in strain VBW088(T) consisted of phosphatidylethanolamine, one unidentified aminolipid and three unidentified phospholipids. The DNA G+C content of strain VBW088(T) was 36.7 mol%. On the basis of phylogenetic inference, DNA-DNA relatedness, chemotaxonomic analysis and physiological data, the isolate represents a novel species of the genus Vitellibacter, for which the name Vitellibacter nionensis sp. nov. is proposed, with the type strain as VBW088(T). (=KCTC 32420(T)=MCC 2354(T)).</p>1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aYoon, Jung-Hoon1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana  uhttps://library.ncl.res.in/content/vitellibacter-nionensis-sp-nov-isolated-shallow-water-hydrothermal-vent-0