02131nas a2200265 4500008004100000022001400041245011800055210006900173260000800242300001000250490000800260520122700268653002501495653001701520653002101537653002701558653001601585653001001601100002501611700002301636700002501659700002301684700002701707856013101734 2021 eng d a0168-165600aRhizobacterial consortium mediated aroma and yield enhancement in basmati and non-basmati rice ( Oryza sativa L.)0 aRhizobacterial consortium mediated aroma and yield enhancement i cFEB a47-580 v3283 a

Basmati and non-basmati rice varieties are commercially important. Aromatic rice varieties are low yielding and recently depletion in aroma is observed due to the shift towards modern agriculture. Therefore, it is necessary to restore the aroma and increase the yield through sustainable agriculture. The use of microbial bioinoculants is one of the promising ways to achieve these targets. With these objectives, rhizospheric bacterial strains Enterobacter hormaechei (AM122) and Lysinibacillus xylanilyticus (DB25) having the property of synthesizing 2acetyl-1-pyrroline (2AP) were isolated from the rhizosphere of two aromatic rice varieties, Ambemohar-157 and Dehradun Basmati respectively and their effect on plant growth, aroma and yield enhancement under mono-inoculation and consortium conditions was analyzed. The bacterial inoculum in consortium resulted in significant improvement in vegetative growth, yield and 2AP content over mono inoculation and control. The study highlights the potential of E. hormaechei and L. xylanilyticus in plant growth, yield and aroma enhancement in basmati and non-basmati rice varieties. These strains can be taken up further for developing a commercial bioformulation.

10a2-Acetyl-1-pyrroline10aBasmati rice10aNon-basmati rice10aplant growth promotion10aRhizosphere10ayield1 aDhondge, Harshal, V.1 aPable, Anupama, A.1 aBarvkar, Vitthal, T.1 aDastager, Syed, G.1 aNadaf, Altafhusain, B. uhttps://library.ncl.res.in/content/rhizobacterial-consortium-mediated-aroma-and-yield-enhancement-basmati-and-non-basmati-rice02726nas a2200277 4500008004100000022001400041245011000055210006900165260000800234300001100242490000800253520182900261653001502090653002402105653001502129653001702144653001602161653000902177100002102186700002502207700001702232700002302249700002302272700002702295856012602322 2022 eng d a0944-501300aExploring the core microbiota in scented rice (Oryza sativa L.) rhizosphere through metagenomics approach0 aExploring the core microbiota in scented rice Oryza sativa L rhi cOCT a1271570 v2633 a

Rice is a major food crop cultivated around the globe. Specially scented rice varieties are of commercial importance but they are low-yielding. The rhizospheric microflora plays a significant role in improving yield and aroma. However, the core microbiome of the scented rice rhizosphere is comparatively less explored. Here, we analyzed the core microbiome associated with the rhizosphere of the scented (Ambemohar-157 and Dehradun basmati) in comparison with non-scented rice (Kolam and Arize 6444 Gold) cultivated at two different geoclimatic zones of India (Maharashtra and Uttarakhand) using the metagenomics approach. The alpha and beta diversity analysis showed that the microbial communities associated with scented and non-scented varieties significantly changes with respect to richness, diversity, and evenness. The taxonomic profiling revealed the variation in composition, diversity, and abundance of the microbiome in terms of phyla and genera associated with scented rice varieties over non-scented. The cluster analysis distinguishes the microbial communities based on their geographical positions. The core microbiome analysis revealed that scented rice rhizosphere shelters distinct and unique microbiota. 28.6 % of genera were exclusively present only in the scented rice rhizosphere. The putative functional gene annotation revealed the high abundance of genes related to the biosynthesis of 2-acetyl-1-pyrroline (2AP) precursors in scented rice. The precursor feeding analysis revealed proline as a preferred substrate by 2AP synthesizing bacteria. The 2AP precursor proline and proline metabolism genes showed a positive correlation. The scented rice-specific rhizobacteria pointed out in this study can be used as bioinoculants for enhancing aroma, yield, and sustainable rice cultivation.

10aMetagenome10aMicrobial community10aMicrobiome10aOryza sativa10aRhizosphere10arice1 aDhondge, Harshal1 aBarvkar, Vitthal, T.1 aPaul, Dhiraj1 aDastager, Syed, G.1 aPable, Anupama, A.1 aNadaf, Altafhusain, B. uhttps://library.ncl.res.in/content/exploring-core-microbiota-scented-rice-oryza-sativa-l-rhizosphere-through-metagenomics02526nas a2200277 4500008004100000022001400041245010300055210006900158260000800227300000700235490000700242520162300249653001801872653001301890653001301903653002301916653002001939653002701959100001601986700002302002700002402025700002502049700002102074700002702095856012602122 2023 eng d a0137-588100aRole of ions and their transporters in combating salt stress in Pandanus odorifer (Forssk.) Kuntze0 aRole of ions and their transporters in combating salt stress in  cMAY a660 v453 a

Soil salinity is a major environmental constraint causing huge losses in crop production. Pandanus odorifer (Forssk.) Kuntze is an aromatic monocot plant species belonging to the family Pandanaceae, which grows naturally on the saline soils along the coasts of Asia, Southeast Asia, and Polynesia. The plants are dioecious and highly fertile, and the consistent saline sprays, strong winds, and severe soil salinity hardly affect their growth and fertility. Hence, understanding the mechanism of high salinity tolerance in P. odorifer could provide major advances in overcoming salinity stress in crop plants. In the present study, we analyzed control (0 M NaCl) and treated (1 M NaCl treatment for 3 weeks) plants of P. odorifer to understand the role of ion distribution, ion transport, and related mechanisms under salt stress. Using a combination of ionomics and transcriptomics approaches, we identified the molecular mechanisms contributing to the high salinity stress in P. odorifer. Under NaCl stress, there was a significant increase in Na, Cl, and other ions in leaves, while the concentrations of Si, Fe, Ni, and Ti decreased. Similarly, in roots, the levels of Na, Mg, Cd, and Cr were significantly high, while the levels of other ions decreased. Most of the genes related to ion transport and homeostasis, such as NHX1, CLC-C, SOS1, HAK, and ABC transporters, were upregulated in 1 M NaCl stress conditions. This study revealed that vacuolar sequestration of Na+ and the distribution of ions in the roots and shoots play significant roles in the salt-stress tolerance mechanism of P. odorifer.

10aIon transport10aIonomics10aPandanus10aSalinity tolerance10aTranscriptomics10aVacuolar sequestration1 aRashmi, Deo1 aAnsari, Waquar, A.1 aKadoo, Narendra, Y.1 aBarvkar, Vitthal, T.1 aDeshmukh, Rupesh1 aNadaf, Altafhusain, B. uhttps://library.ncl.res.in/content/role-ions-and-their-transporters-combating-salt-stress-pandanus-odorifer-forssk-kuntze02902nas a2200301 4500008004100000022001400041245009800055210006900153260000900222300001100231490000800242520188100250653002502131653002402156653003702180653002702217653001802244653002602262100002502288700002502313700002302338700002302361700001802384700002302402700002202425700002702447856012602474 2024 eng d a0168-160500aGenome sequencing and protein modeling unraveled the 2AP biosynthesis in Bacillus cereus DB250 aGenome sequencing and protein modeling unraveled the 2AP biosynt cMAR  a1106000 v4133 a

2-Acetyl-1-pyrroline (2AP) is an important and major flavor aroma compound responsible for the fragrance of basmati rice, cheese, wine, and several other food products. Biosynthesis of 2AP in aromatic rice and a few other plant species is associated with a recessive Betaine aldehyde dehydrogenase 2 (BADH2) gene. However, the literature is scant on the relationship between the functional BADH2 gene and 2AP biosynthesis in prokaryotic systems. Therefore, in the present study, we aimed to explore the functionality of the BADH2 gene for 2AP biosynthesis in 2AP synthesizing rice rhizobacterial isolate Bacillus cereus DB25 isolated from the rhizosphere of basmati rice (Oryza sativa L.). Full-length BcBADH2 sequence was obtained through whole genome sequencing (WGS) and further confirmed through traditional PCR and Sanger sequencing. Then the functionality of the BcBADH2 gene was evaluated in-silico through bioinformatics analysis and protein docking studies and further experimentally validated through enzyme assay. The sequencing and bioinformatics analysis results revealed a full-length 1485 bp BcBADH2 coding sequence without any deletion or premature stop codons. Full-length BcBADH2 was found to encode a fully functional protein of 54.08 kDa with pI of 5.22 and showed the presence of the conserved amino acids responsible for enzyme activity. The docking studies confirmed a good affinity between the protein and its substrate whereas the presence of BcBADH2 enzyme activity confirmed the functionality of BADH2 enzyme in B. cereus DB25. In conclusion, the findings of the present study suggest that B. cereus DB25 is able to synthesize 2AP despite a functional BADH2 gene and there may be a different molecular mechanism responsible for 2AP biosynthesis in bacterial systems, unlike that found in aromatic rice and other eukaryotic plant species.

10a2-Acetyl-1-pyrroline10aBasmati rice flavor10aBetaine aldehyde dehydrogenase 210aProtein-ligand docking10aRhizobacteria10aWhole-genome sequence1 aDhondge, Harshal, V.1 aBarvkar, Vitthal, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S.1 aRajput, Vinay1 aPable, Anupama, A.1 aHenry, Robert, J.1 aNadaf, Altafhusain, B. uhttps://library.ncl.res.in/content/genome-sequencing-and-protein-modeling-unraveled-2ap-biosynthesis-bacillus-cereus-db2502558nas a2200253 4500008004100000022001400041245009000055210006900145260000800214300001200222490000700234520173800241653001901979653001301998653001802011653002302029653001602052653002602068100001602094700002502110700002702135700002402162856011802186 2025 eng d a0971-781100aHarnessing microRNAs and their targets for salt stress tolerance in Pandanus odorifer0 aHarnessing microRNAs and their targets for salt stress tolerance cSEP a714-7300 v343 a

Salinity poses a formidable challenge to agriculture, detrimentally impacting plant growth and the yield of several crops globally. Pandanus odorifer (Forssk.) Kuntze is a highly salt-stress resilient plant that grows naturally along the coastal regions of Asia, Southeast Asia, and Polynesia. It is frequently found as a frontier species along the sandy shores receiving constant salt sprays. MicroRNAs (miRNAs) play essential regulatory roles in growth, development, and combating various biotic and abiotic stresses. Thus, characterizing miRNAs and their targets is critical to understanding their roles in salt stress tolerance. Through a rigorous analysis based on homology, secondary structure and signatures of other miRNA genes, we identified 52 conserved nonredundant miRNAs from the P. odorifer transcriptome data. These miRNAs were diverse and belonged to 23 miRNA phylogenetic families. Several miRNAs, including pod-MIR156a, pod-MIR164d, and pod-MIR535d, demonstrated distinct expression patterns in response to salt stress. Their target genes exhibited opposing expression trends, corroborating the intricate miRNA-target relationship. Delving deeper into their influence, we discovered that these miRNAs mainly targeted transcription factors that are known for their pivotal roles in stress responses. We also detected four miRNA-star (miRNA*) sequences (the complementary strands of functional mature miRNAs), which supports the authenticity of the identified miRNAs. This study unveils the intricate regulatory mechanism through which miRNAs may reinforce salt stress tolerance in P. odorifer, providing a promising avenue for enhancing crop resilience to various stresses through miRNA-based strategies.

10aAbiotic stress10amicroRNA10amiRNA targets10aSalinity tolerance10asalt stress10aTranscription factors1 aRashmi, Deo1 aBarvkar, Vitthal, T.1 aNadaf, Altafhusain, B.1 aKadoo, Narendra, Y. uhttps://library.ncl.res.in/content/harnessing-micrornas-and-their-targets-salt-stress-tolerance-pandanus-odorifer