01574nas a2200265   4500008004100000022001400041245011300055210006900168260000900237300001000246490000700256520067800263653002800941653001300969653001900982653001401001653001301015653002401028100001601052700002201068700003101090700002701121700003001148856013001178       2020                        eng d  a1751-825300aOvalbumin-mediated synthesis and simultaneous functionalization of graphene with increased protein stability0 aOvalbuminmediated synthesis and simultaneous functionalization o  cJAN   a60-670 v133 a<p>In this article, we have shown an easy, one-step, protein-directed approach for the synthesis of water-soluble and functional graphene using ovalbumin. The obtained ovalbumin functionalized graphene was characterized by UV-visible spectroscopy, X-ray diffraction, Raman spectroscopy, and Fourier transform infrared spectroscopy. Atomic force microscopy was used to check the attachment/functionalization of ovalbumin on grapheme sheets. It was shown that the functionalization of ovalbumin on grapheme sheets makes the protein more thermally stable compare to free ovalbumin, which has been shown by using sodium dodecyl sulfate polyacrylamide gel electrophoresis.</p>
10aAtomic force microscopy10agraphene10agraphene oxide10aOvalbumin10aSDS-PAGE10athermal degradation1 aUllah, Riaz1 aKhan, Shadab, Ali1 aAladresi, Aref, Ali Mohamm1 aAlharbi, Sulaiman, Ali1 aChinnathambi, Arunachalam  uhttps://library.ncl.res.in/content/ovalbumin-mediated-synthesis-and-simultaneous-functionalization-graphene-increased-protein02298nas a2200325   4500008004100000022001400041245010500055210006900160260000800229300001400237490000700251520126500258653001201523653002601535653001401561653002101575653003001596653002201626100002001648700002101668700002101689700001701710700001901727700002001746700001901766700002101785700001601806700001901822856013101841       2022                        eng d  a2001-037000aSNP discovery and structural insights into OeFAD2 unravelling high oleic/linoleic ratio in olive oil0 aSNP discovery and structural insights into OeFAD2 unravelling hi  cJUL  a1229-12430 v203 a<p>
	Fatty Acid Desaturase 2 (FAD2), a key enzyme in the fatty acid biosynthesis pathway, is involved in the desaturation and conversion of oleic acid to linoleic acid. Therefore, it plays a crucial role in oleic/linoleic acid ratio and the quality of olive oil. DNA sequencing of 19 FAD2 genes from a set of olive oil varieties revealed several single-nucleotide polymorphisms (SNPs) and highlighted associations between some of the SNPs and saturated fatty acids contents. This was further confirmed by SNP-interaction and machine learning approach. Haplotype diversity analysis led to the discovery of three highly polymorphic SNPs and four haplotypes harboring differential oleic/linoleic acid ratios. Moreover, a combination of molecular modeling and docking experiments allowed a deeper and better understanding of the structure-function relationship of the FAD2 enzyme. Sequence patterns and variations involved in the regulation of the FAD2 activity were also identified. Furthermore, S82C and H213N substitutions in OeFAD2 make the Oueslati variety more interesting in terms of fatty acid profile and oleic acid level. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.</p>
10adocking10aFatty acid desaturase10aHaplotype10amachine learning10aOleic/linoleic acid ratio10aProtein structure1 aBen Ayed, Rayda1 aMoreau, Fabienne1 aBen Hlima, Hajer1 aRebai, Ahmed1 aErcisli, Sezai1 aKadoo, Narendra1 aHanana, Mohsen1 aAssouguem, Amine1 aUllah, Riaz1 aAli, Essam, A.  uhttps://library.ncl.res.in/content/snp-discovery-and-structural-insights-oefad2-unravelling-high-oleiclinoleic-ratio-olive-oil