01496nas a2200193   4500008004100000022001400041245018600055210006900241260000900310300001200319490000600331520072700337100002301064700001801087700002001105700002601125700002401151856012701175       2020                        eng d  a2470-134300aMethoxymethyl threofuranosyl thymidine (4 `-MOM-TNA-T) at the T7 position of the thrombin-binding aptamer boosts anticoagulation activity, thermal stability, and nuclease resistance0 aMethoxymethyl threofuranosyl thymidine 4 MOMTNAT at the T7 posit  cJAN   a498-5060 v53 a<p>The synthesis of 4'-methoxymethyl threofuranosyl (4'-MOM-TNA) thymidine and derived oligomers of the G-rich thrombin-binding aptameric (TBA) sequence is reported. The G-quadruplex stability, anticoagulation activity, and the enzymatic stability of these oligomers bearing the 2'-3'-phosphodiester backbone as single substitutions in the loop regions are studied. Amongst all the oligomers, TBA-7T bearing the 4'-MOM-TNA unit at the T7 position formed a quadruplex with the highest thermal stability. It also resulted in enhanced anticlotting activity that allowed a one-third reduction in the dose, relative to TBA. Further, TBA-7T exhibited enhanced nuclease resistance properties to both endo- and exonucleases.</p>
1 aVarada, Manojkumar1 aAher, Manisha1 aErande, Namrata1 aKumar, Vaijayanti, A.1 aFernandes, Moneesha  uhttps://library.ncl.res.in/content/methoxymethyl-threofuranosyl-thymidine-4-mom-tna-t-t7-position-thrombin-binding-aptamer