02789nas a2200289   4500008004100000022001400041245015800055210006900213260000800282300001400290490000700304520175600311653002102067653001702088653003302105653002102138653002002159100002502179700002102204700003202225700002202257700002002279700002502299700002202324700002402346856012902370       2020                        eng d  a0721-759500aEnzymatic and non-enzymatic detoxification of reactive carbonyl compounds improves the oxidative stress tolerance in cucumber, tobacco and rice seedlings0 aEnzymatic and nonenzymatic detoxification of reactive carbonyl c  cSEP  a1359-13720 v393 a<p>Detoxification of reactive carbonyl compounds (RCC) is crucial to sustain cellular activity to improve plant growth and development. Seedling growth is highly affected by accumulation of RCC under stress. We report non-enzymatic, enzymatic mechanisms of detoxification of RCC in the cucumber, tobacco and rice seedling systems exposed to glucose, NaCl, methyl viologen (MV) induced oxidative stress. The cucumber seedlings exposed to carbonyl stress had higher levels of malondialdehyde (MDA), protein carbonyls (PCs) and advanced glycation end-product N-carboxymethyl-lysine (AGE-CML) that negatively affected the seedling growth. The overexpression of enzyme encoding aldo-keto reductase-1 (AKR1) in tobacco and rice showed detoxification of RCC, MDA and methylglyoxal (MG) with improved seedling growth under glucose, NaCl and MV-induced oxidative stress. Further, small molecules like acetylsalicylic acid (ASA), aminoguanidine (AG), carnosine (Car), curcumin (Cur) and pyridoxamine (PM) showed detoxification of RCC non-enzymatically and rescued the cucumber seedling growth from glucose, NaCl and MV-stress. In autotrophically grown rice seedlings these molecules substantially improved seedling growth under MV-induced oxidative stress. Seedlings treated with the small molecules sustained higher guaiacol peroxidase (GPX) enzyme activity signifying the role of small molecules in reducing carbonyl stress-induced protein inactivation and AGE-CML protein modifications. The results showed that besides enzymatic detoxification of RCC, the small molecules also could reduce cytotoxic effect of RCC under stress. The study demonstrates that small molecules are attractive compounds to improve the seedling growth under stress conditions.</p>
10aOxidative stress10aplant growth10aReactive carbonyl scavengers10aSeed germination10asmall molecules1 aNareshkumar, Ambekar1 aSubbarao, Sindhu1 aVennapusa, Amarnatha, Reddy1 aAshwin, Vargheese1 aBanarjee, Reema1 aKulkarni, Mahesh, J.1 aRamu, Vemanna, S.1 aUdayakumar, Makarla  uhttps://library.ncl.res.in/content/enzymatic-and-non-enzymatic-detoxification-reactive-carbonyl-compounds-improves-oxidative