02492nas a2200277   4500008004100000022001400041245016500055210006900220260000800289300001100297490000800308520149600316653001801812653001201830653001801842653002701860100002101887700002701908700002301935700002501958700002701983700002302010700003102033700002602064856012402090       2020                        eng d  a1381-514800aEpoxy functionalized polymer grafted magnetic nanoparticles by facile surface initiated polymerization for immobilization studies of Candida Antarctica lipase B0 aEpoxy functionalized polymer grafted magnetic nanoparticles by f  cFEB  a1044540 v1473 a<p>Immobilization of Candida Antarctica lipase B (Cal-B) was done on the epoxy functionalized polymer (EFP) grafted magnetic nanoparticles (MNPs) via covalent attachment with the active epoxy groups. The EFP brushes were grafted on iron-oxide based MNPs by a facile surface-initiated atom transfer radical polymerization (ATRP) using activators generated by electron transfer (AGET) of glycidyl methacrylate (GMA). Each step of the surface modification, polymer grafting, and enzyme immobilization process on the polymer grafted MNPs was studied using Fourier transform infrared spectroscopy (FTIR). A thermogravimetric analysis (TGA) calculated the amount of engineered organic components, a transmission electron microscopy (TEM) visualized the core-shell formation of the MNPs, and a vibrating sample magnetometer (VSM) validated their magnetic properties at various modification stages. The lipase immobilization efficiency was described as a function of immobilization time, as well as, enzyme amount. The activity was characterized within a range of pH, temperature, kinetic parameters, resusability and storage stability, for both the free and immoblized Cal-B enzyme. The results of this study suggested that poly(GMA) grafted MNPs can be successfully used for the immobilization of Cal-B with improved efficiencies compared to those obtained with free soluble lipase. The reported enzyme immobilization method appears to be reproducible and scalable for industrial production.</p>
10aCal-B enzymes10aenzymes10aImmoblization10aMagnetic nanoparticles1 aAfzal, Haq, Asif1 aGhorpade, Ravindra, V.1 aThorve, Asmita, K.1 aNagaraja, Sreeharsha1 aAl-Dhubiab, Bandar, E.1 aMeravanige, Girish1 aRasool, Sahibzada, Tasleem1 aRoopashree, Teeka, S.  uhttps://library.ncl.res.in/content/epoxy-functionalized-polymer-grafted-magnetic-nanoparticles-facile-surface-initiated