02428nas a2200241   4500008004100000022001400041245015600055210006900211260000800280300000900288490000700297520153600304100002101840700002301861700001901884700002701903700002601930700002001956700002701976700002802003700002802031856012702059       2020                        eng d  a2045-232200aHighly sensitive label-free bio-interfacial colorimetric sensor based on silk fibroin-gold nanocomposite for facile detection of chlorpyrifos pesticide0 aHighly sensitive labelfree biointerfacial colorimetric sensor ba  cMAR  a41980 v103 a<p>Herein, the preparation of gold nanoparticles-silk fibroin (SF-AuNPs) dispersion and its label-free colorimetric detection of the organophosphate pesticide, namely chlorpyrifos, at ppb level are reported. The silk fibroin solution was extracted from B. mori silk after performing degumming, dissolving and dialysis steps. This fibroin solution was used for synthesis of gold nanoparticles in-situ without using any external reducing and capping agent. X-ray Diffractometry (XRD), Field Emission Transmission Electron Microscopy (FETEM) along with Surface Plasmon Resonance based optical evaluation confirmed generation of gold nanoparticles within SF matrix. The resultant SF-AuNPs dispersion exhibited rapid and excellent colorimetric pesticide sensing response even at 10 ppb concentration. Effect of additional parameters viz. pH, ionic concentration and interference from other pesticide samples was also studied. Notably, SF-AuNPs dispersion exhibited selective colorimetric pesticide sensing response which can be calibrated. Furthermore, this method was extended to various simulated real life samples such as tap water, soil and agricultural products including plant residues to successfully detect the presence of chlorpyrifos pesticide. The proposed colorimetric sensor system is facile yet effective and can be employed by novice rural population and expert researchers alike. It can be exploited as preliminary tool for label-free colorimetric chlorpyrifos pesticide sensing in water and agricultural products.</p>
1 aMane, Pramod, C.1 aShinde, Manish, D.1 aVarma, Sanjana1 aChaudhari, Bhushan, P.1 aFatehmulla, Amanullah1 aShahabuddin, M.1 aAmalnerkar, Dinesh, P.1 aAldhafiri, Abdullah, M.1 aChaudhari, Ravindra, D.  uhttps://library.ncl.res.in/content/highly-sensitive-label-free-bio-interfacial-colorimetric-sensor-based-silk-fibroin-gold04223nas a2200277   4500008004100000022001400041245011700055210006900172260000800241300000700249490000700256520327800263653002603541653002303567653002603590653002103616653001303637100002103650700002303671700002203694700002603716700002403742700002403766700002803790856012703818       2023                        eng d  a1868-695800aGreen adeptness in synthesis of non-toxic copper and cobalt oxide nanocomposites with multifaceted bioactivities0 aGreen adeptness in synthesis of nontoxic copper and cobalt oxide  cDEC  a790 v143 a<p>
	Background: In the present era, we are facing different health problems mainly concerning with drug resistance in microorganisms as well as in cancer cells. In addition, we are also facing the problems of controlling oxidative stress and insect originated diseases like dengue, malaria, chikungunya, etc. originated from mosquitoes. In this investigation, we unfurled the potential of Achatina fulica mucus in green synthesis of mucus mediated copper oxide bio-nanocomposites (SM-CuONC) and cobalt oxide bio-nanocomposites (SM-Co3O4NC). Herein we carried out the physico-chemical characterization like UV-Vis spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Transmission electron microscopy (TEM), Energy Dispersive X-ray Analysis (EDAX) and X-ray photoelectron spectroscopy (XPS) of as synthesized bio-nanocomposites. Both the bio-nanocomposites were tested for their potential as antimicrobial activity using well diffusion assay, anticancer activity by MTT assay, antioxidant activity by phosphomolybdenum assay and mosquito larvicidal activity.Results: The results of this study revealed that, SM-CuONC and SM-Co3O4NC were synthesized successfully using A. fulica mucus. The FESEM and TEM data reveal the formation of nanoparticles with quasi-spherical morphology and average particle size of similar to 18 nm for both nanocomposites. The EDAX peak confirms the presence of elemental copper and cobalt in the analyzed samples. The X-ray diffraction analysis confirmed the crystalline nature of the CuO and Co3O4. The result of anti microbial study exhibited that, SM-CuONC showed maximum antimicrobial activity against Escherichia coli NCIM 2065 and Aspergillus fumigatus NCIM 902 which were noted as 2.36 +/- 0.31 and 2.36 +/- 0.59 cm resp. at 60 mu g/well concentration. The result of anticancer activity for SM-CuONC was exhibited as, 68.66 +/- 3.72, 62.66 +/- 3.61 and 71.00 +/- 2.36 percent kill, while SM-Co3O4NC exhibited 61.00 +/- 3.57, 72.66 +/- 4.50 and 71.66 +/- 4.22 percent kill against Human colon cancer (HCT-15), Cervical cancer (HeLa), and Breast cancer (MDA-MB-231) cell lines, respectively, at 20 mu g/well concentration. Both the nanocomposites also exhibited better antioxidant activity. Total antioxidant activity for SM-CuONC at 50 mu g/ml concentration was found to be highest as 55.33 +/- 3.72 while that of SM-Co(3)O(4)Ns was 52.00 +/- 3.22 mM of ascorbic acid/mu g respectively. Both bio-nanocomposites also exhibited 100% mosquito larvicidal activity at concentration ranging from 40 to 50 mg/l. During cytotoxicity study it is noted that at 5 mu g/well concentration, SM-CuO and SM-Co3O4NCs suspension showed more than 97% viability of normal (L929) cell lines. We also studied phytotoxicity of both bio-nanocomposites on Triticum aestivum. In this study, 100% seed germination was observed when seeds are treated with SM-CuONC and SM-Co3O4NC at 500 mg/l and 250 mg/l concentration respectively.Conclusions: This study concludes that in future as synthesized SM-CuONC and SM-Co3O4NC can be used in pharmaceutical, health care system for betterment and welfare of human life as both bio-nanocomposites exhibits better antimicrobial, anticancer, antioxidant and mosquito larvicidal potential.</p>
10aAchatina fulica mucus10aBio-nanocomposites10aBiological activities10aCharacterization10aToxicity1 aMane, Pramod, C.1 aKadam, Deepali, D.1 aKhadse, Ashok, N.1 aChaudhari, Aditya, R.1 aUghade, Supriya, P.1 aAgawane, Sachin, B.1 aChaudhari, Ravindra, D.  uhttps://library.ncl.res.in/content/green-adeptness-synthesis-non-toxic-copper-and-cobalt-oxide-nanocomposites-multifaceted