03031nas a2200289   4500008004100000022001400041245012300055210006900178260000800247300001100255490000800266520207100274653001802345653002302363653001502386653002002401653001402421653002302435100002502458700001702483700002202500700001902522700002202541700002802563700001802591856013202609       2020                        eng d  a0928-493100aPoly (vinylpyrrolidone)-iodine engineered poly (epsilon-caprolactone) nanofibers as potential wound dressing materials0 aPoly vinylpyrrolidoneiodine engineered poly epsiloncaprolactone   cMAY  a1107310 v1103 a<p>Facilitating the process of wound healing and effective treatment of wounds remains a serious challenge in healthcare. Wound dressing materials play a major role in the protection of wounds and in accelerating the natural healing process. In the present study, novel core/shell (c/s) nanofibrous mats of poly(vinyl pyrrolidone)-iodine (PVPI) and polycaprolactone (PCL) were fabricated using a co-axial electrospinning process followed by their surface modification with poly-L-lysine. The developed nanofibrous mats were extensively characterized for their physicochemical properties using various analytical techniques. The core/shell structure of the PVP-I/PCL nanofibers was confirmed using TEM analysis. The PVP-I release studies showed an initial burst phase followed by a sustained release pattern of PVP-I over a period of 30 days. The developed nanofibers exhibited higher BSA and fibrinogen adsorption as compared to pristine PCL. Cytotoxicity studies using MTT assay demonstrated that the PVP-I/PCL (c/s) nanofibers were cytocompatible at optimized PVP-I concentration (3 wt%). The PCL-poly-L-lysine and PVP-I/PCL-poly-L-lysine nanofibers exhibited higher cell viability (24.2% and 21.4% higher at day 7) when compared to uncoated PCL and PVP-I/PCL nanofibers. The PVP-I/PCL nanofibers showed excellent antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria. The inflammatory response of Mouse RAW 264.7 macrophage cells towards the nanofibers was studied using RTPCR. It revealed that the pro-inflammatory cytokines (TNF-alpha and IL-1 beta) were significantly upregulated on PCL nanofibers, while their expression was comparatively lower on poly-L-lysine coated PCL or PVP-I/PCL(c/s) nanofibers. Overall, the study highlights the ability of poly-L-lysine coated PVP-I/PCL (c/s) nanofibers as potential wound dressing materials effectively facilitating the early stage wound healing and repair process by virtue of their selective modulation of inflammation, cell adhesion and antimicrobial properties.</p>
10abiodegradable10aControlled release10aCore/shell10aelectrospinning10ascaffolds10atissue engineering1 aShitole, Ajinkya, A.1 aRaut, Piyush1 aGiram, Prabhanjan1 aRade, Priyanka1 aKhandwekar, Anand1 aGarnaik, Baijayantimala1 aSharma, Neeti  uhttps://library.ncl.res.in/content/poly-vinylpyrrolidone-iodine-engineered-poly-epsilon-caprolactone-nanofibers-potential-wound