02693nas a2200217   4500008004100000022001400041245019300055210006900248260000800317300001400325490000700339520181800346100003502164700002102199700002902220700002602249700002102275700002402296700002702320856012802347       2020                        eng d  a1144-054600aElectrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/polyethylene oxide (PEO) microfibers reinforced with ZnO nanocrystals for antibacterial and antibiofilm wound dressing applications0 aElectrospun poly3hydroxybutyrateco3hydroxyvaleratepolyethylene o  cJUN  a9754-97660 v443 a<p>Biocompatible and biodegradable polymers are extensively used in designing wound dressing materials. The present investigation deals with the preparation of a unique blend of zinc oxide (ZnO) nanoparticles incorporated in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/polyethylene oxide (PEO) microfibersviaan electrospinning technique for antibacterial, antibiofilm and wound dressing applications. This composite was prepared by incorporating previously synthesized ZnO NPs with better antibacterial and antibiofilm activity in PHBV-PEO (4 : 1) polymers in chloroform solution. Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy confirmed that ZnO NPs were incorporated in the PHBV-PEO microfibers. The synthesized microfibers exhibited enhanced mechanical properties after the incorporation of ZnO NPs. The results of antibacterial and antibiofilm activity of the prepared microfibers revealed that the incorporated ZnO NPs in different concentrations (1%, 3%, and 5%) showed different degrees of antibacterial activity against pathogenic Gram-positiveStaphylococcus aureus(NCIM 2654) and Gram-negativePseudomonas aeruginosa(NCIM 5032), which are the main bacteria found in wound infections. The PHBV-PEO-ZnO microfibers exhibited excellent hemocompatibility with improved swelling behavior after the incorporation of ZnO NPs.In vitrocytotoxicity assays revealed the non-toxic nature of the prepared PHBV-PEO-ZnO microfibers. The current work confirms that utilizing a unique blend of the biodegradable, biocompatible, thermoplastic and hydrophobic natural polymer PHBV with hydrophobic, biodegradable, non-toxic and synthetic polymer PEO with ZnO NPs holds great potential for use as an antibacterial and antibiofilm material for wound dressing applications.</p>
1 aMahamuni-Badiger, Pranjali, P.1 aPatil, Pooja, M.1 aPatel, Pratikshkumar, R.1 aDhanavade, Maruti, J.1 aBadiger, Manohar1 aMarathe, Yogesh, N.1 aBohara, Raghvendra, A.  uhttps://library.ncl.res.in/content/electrospun-poly3-hydroxybutyrate-co-3-hydroxyvaleratepolyethylene-oxide-peo-microfibers