01191nas a2200229   4500008004100000022001400041245014500055210006900200260000800269520029900277653003800576653002900614653000800643653003300651653003400684100002500718700002100743700002000764700002300784700002500807856012900832       2020                        eng d  a0739-110200aEvaluation of biomolecular interactions and cytotoxic activity of organometallic binuclear Ru(II) complexes of ferrocenyl thiosemicarbazones0 aEvaluation of biomolecular interactions and cytotoxic activity o  cJUL3 a<p>{Four new ferrocenyl substituted thiosemicarbazone ligands (L1-L4) and their corresponding binuclear ruthenium(II) arene complexes of the general type [(eta(6)-pcym)(L)Ru(mu-im)Ru(L)(eta(6)-p-cym)]Cl (C1-C4) and [(eta(6)-pcym)(L)Ru(mu-azpy)Ru(L)(eta(6)-p-cym)]Cl-2(C5-C8) (cym = cymene</p>
10abinuclear ruthenium(II) complexes10aBSA binding interactions10aDNA10aFerrocenyl thiosemicarbazone10aHeLa human cervical carcinoma1 aKhanvilkar, Priyanka1 aDash, Soumya, R.1 aVohra, Alisagar1 aDevkar, Ranjitsinh1 aChakraborty, Debjani  uhttps://library.ncl.res.in/content/evaluation-biomolecular-interactions-and-cytotoxic-activity-organometallic-binuclear-ruii02096nas a2200265   4500008004100000022001400041245015300055210006900208260000800277490000700285520111600292653003901408653002901447653002101476653000801497653002701505653003401532100002501566700002101591700002301612700002101635700002301656700002501679856012601704       2021                        eng d  a1735-207X00aImidazole/4,4 `-azopyridine bridging binuclear Ru(II) complexes: design, synthesis, bimolecular interactions and cytotoxicity against HeLa cell line0 aImidazole44 azopyridine bridging binuclear RuII complexes design  cDEC0 v183 a<p>Binuclear Ru(II)-arene complexes [(eta(6)-pcym)(Flq)Ru(mu-im/mu-azpy)Ru(Flq)(eta(6)-p-cym)]Cl (C1-C8) (cym = cymene; Flq = fluoroquinolones; im = imidazole; azpy = 4,4 ` azo pyridine) have been synthesized and characterized by elemental analysis, molar conductivity and various spectral techniques (ESI-MS, IR, UV-Vis and H-1-NMR). The geometry of the complexes was optimized by DFT calculations, which revealed a pseudo-octahedral coordination around each metal centre. Binding of the synthesized complexes with CT-DNA and BSA was studied spectroscopically, and it has been established that the presence of two hydrophobic planar arene moieties enhances the binding efficacies of the binuclear complexes to the macromolecules, compared to their mononuclear analogues. The results of competitive binding between C1-C8 and ethidium bromide (EB) towards DNA have shown that the complexes are able to displace EB from DNA-EB adduct and interact with DNA via intercalation. The complexes display cytotoxicity against the HeLa human cervical cancer cell lines with IC50 values in the range of 30.1-120.9 mu M.</p>10aBinuclear ruthenium (II) complexes10aBSA binding interactions10aDFT calculations10aDNA10aFluoroquinolones (FQs)10aHeLa human cervical carcinoma1 aKhanvilkar, Priyanka1 aDash, Soumya, R.1 aPulipaka, Ramadevi1 aShirsath, Kavita1 aDevkar, Ranjitsinh1 aChakraborty, Debjani  uhttps://library.ncl.res.in/content/imidazole44-azopyridine-bridging-binuclear-ruii-complexes-design-synthesis-bimolecular02563nas a2200265   4500008004100000022001400041245016500055210006900220260000800289300001000297490000700307520156800314653003901882653002901921653000801950653003401958653003901992100002502031700002102056700002202077700002002099700002302119700002502142856013002167       2021                        eng d  a0268-260500aOrganoruthenium (II) complexes featuring pyrazole-linked thiosemicarbazone ligands: synthesis, DNA/BSA interactions, molecular docking, and cytotoxicity studies0 aOrganoruthenium II complexes featuring pyrazolelinked thiosemica  cOCT  ae63430 v353 aA series of pyrazol-derived thiosemicarbazone ligands (L1-L4) were synthesized and reacted with [Ru(p-cymene)(mu-Cl)Cl](2) to yield a series of ``piano-stool''-type binuclear ruthenium (II)-arene-thiosemicarbazone complexes (C1-C8) of the general type [(Ru(eta(6)-p-cym)L)(2)(mu-im/azpy)] Cl1-2 (L = diphenylpyrazole thiosemicarbazone; cym = p-cymene; im = imidazole; azpy = 4,4 `-azopyridine). The thiosemicarbazone ligands act as N and S donors binding to the Ru(II) center via the imine nitrogen and the thione sulfur atoms. The complexes were characterized by NMR, FTIR, UV-Vis spectroscopy, and ESI+ mass spectrometry. The binding of the complexes to calf thymus deoxyribonucleic acid (CT-DNA) and bovine serum albumin (BSA) was evaluated, and it has been established that the binuclear complexes have good binding efficacies with DNA (K-b = 10(4)-10(5) M-1) and BSA (K-a = 10(5)-10(6) M-1). This is attributed to the arene moieties present in the ligands of the complexes that can have hydrophobic interactions with DNA/BSA. Ethidium bromide (EB) displacement studies and DNA viscosity measurements revealed intercalative interaction of the complexes with DNA. Static interaction of the complexes with BSA was revealed by fluorescence quenching studies. Molecular docking studies confirmed base stacking, H-bonding, and hydrophobic interactions with the biomolecules. In vitro antiproliferative studies of the complexes affirmed that the complexes are cytotoxic towards the HeLa (human cervical cancer) cell line with IC50 values in range of 17.3-41.3 mu M.10aBinuclear ruthenium (II) complexes10aBSA binding interactions10aDNA10aHeLa human cervical carcinoma10apyrazole-derived thiosemicarbazone1 aKhanvilkar, Priyanka1 aDash, Soumya, R.1 aBanerjee, Devjani1 aVohra, Aliasgar1 aDevkar, Ranjitsinh1 aChakraborty, Debjani  uhttps://library.ncl.res.in/content/organoruthenium-ii-complexes-featuring-pyrazole-linked-thiosemicarbazone-ligands-synthesis