02740nas a2200217   4500008004100000022001400041245015100055210006900206260007100275300001400346490000800360520189600368100001802264700001602282700001402298700001602312700002202328700002602350700001802376856012802394       2009                        eng d  a1520-610600aEffects for the incorporation of five-atom thioacetamido nucleic acid (TANA) backbone on hybridization thermodynamics and kinetics of DNA duplexes0 aEffects for the incorporation of fiveatom thioacetamido nucleic   a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAR  a2944-29510 v1133 a<p>Thermodynamic studies of nucleic acids serve not only to widen our understanding on the nature and strength of forces that stabilize nucleic acids in a myriad of structural states they assume but also to facilitate the development of databases that could be used to predict the stability and selectivity of probe/primer-sets required in a broad range of nucleic acid-based diagnostic and therapeutic protocols. In the current study, we investigated the effect of a novel, backbone-modified ``thioacetamido linkage'' (TANA) on thermodynamics of hybridization, binding kinetics, and conformation of a DNA duplex. The modification comprises of an extended five-atom amide (N3'-CO-CH(2)-S-CH(2)) linker, as opposed to four-atom phosphodiester linker backbone present in DNA. One to three TANA-substitutions have been introduced in the linker backbone of two thymidine residues of one of the strand of the DNA duplex. Using spectroscopic and calorimetric techniques, we observed that TANA destabilizes the DNA helix by lowering the favorable enthalpy parameter of duplex formation. TANA center dot DNA duplexes were found to adopt a conformation toward an A-type duplex as shown by circular dichroism spectroscopy studies. Analysis of differential scanning calorimetry data indicated a nonzero heat capacity change, Delta C(p), accompanying the duplex formation. The average Delta C(p) change per duplex was found to be 832.5 cal mol(-1) K(-1), giving an average base-pair change of 59.5 cal (mol of base pairs)(-1) K(-1). Hybridization kinetic measurements using surface plasmon resonance indicated a decrease in binding affinity parameter (K(A)) that originates from higher dissociation rate constants (k(d)). Furthermore, optical melting studies showed that increasing the number of modifications results in a modest change in the number of counterions taken up during duplex formation.</p>1 aKaur, Harleen1 aArora, Amit1 aGogoi, K.1 aSolanke, P.1 aGunjal, Anita, D.1 aKumar, Vaijayanti, A.1 aMaiti, Souvik  uhttps://library.ncl.res.in/content/effects-incorporation-five-atom-thioacetamido-nucleic-acid-tana-backbone-hybridization-001684nas a2200265   4500008004100000022001400041245011500055210006900170260009900239300001400338490000700352520069900359653001901058653002001077653002201097653002101119653001601140653002301156100001601179700001901195700002601214700002201240700002901262856012701291       2016                        eng d  a0261-306900aEffect of different fixed bed media on the performance of sodium dodecyl sulfate for hydrate based CO2 capture0 aEffect of different fixed bed media on the performance of sodium  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJAN  a1186-11910 v903 a<p>Sodium Dodecyl Sulfate (SDS) is used as a kinetic promoter in gas hydrate formation. In this work, the performance of SDS for carbon dioxide gas hydrate formation in two different fixed bed media: silica sand and zeolite (5A and 13X) has been evaluated. The concentration of SDS was fixed at 0.5 wt%. The experiments were carried out in batch mode with the initial pressure fixed at 3.0 MPa, and the temperature kept constant at 274.65 K. The results showed that hydrate of carbon dioxide with fixed bed of silica sand was significantly promoted by the addition of SDS as compared to the other fixed bed medium used in this study: zeolite 13X. (c) 2015 Elsevier Ltd. All rights reserved.</p>10aCarbon dioxide10aFixed bed media10aHydrate formation10aKinetic promoter10aSilica sand10aZeolite 5A and 13X1 aArora, Amit1 aKumar, Asheesh1 aBhattacharjee, Gaurav1 aKumar, Pushpendra1 aBalomajumder, Chandrajit  uhttps://library.ncl.res.in/content/effect-different-fixed-bed-media-performance-sodium-dodecyl-sulfate-hydrate-based-co2-0