02035nas a2200241   4500008004100000245012600041210006900167260000800236300000800244490000700252520115000259653002501409653002901434653002701463653003101490653001401521653002801535100002701563700002101590700002101611700003001632856013101662       2022                        eng d00aAssembly, annotation, and comparative whole genome sequence of fusarium verticillioides isolated from stored maize grains0 aAssembly annotation and comparative whole genome sequence of fus  cJUL  a8100 v113 a<p>
	Fusarium verticillioides is a plant pathogenic fungus affecting a wide range of crops worldwide due to its toxigenic properties. F. verticillioides BIONCL4 strain was isolated from stored maize grain samples in India, and produces high amount of fumonisin B1 (FB1). We report a comparative genomic analysis of F. verticillioides, covering the basic genome information, secretome, and proteins involved in host-pathogen interactions and mycotoxin biosynthesis. Whole-genome sequencing (WGS) was performed using the Illumina platform with an assembly size of 42.91 Mb, GC content of 48.24%, and 98.50% coverage with the reference genome (GCA000149555). It encodes 15,053 proteins, including 2058 secretory proteins, 676 classical secretory proteins, and 569 virulence and pathogenicity-related proteins. There were also 1447 genes linked to carbohydrate active enzymes (CaZymes) and 167 genes related to mycotoxin production. Furthermore, F. verticillioides genome comparison revealed information about the species' evolutionary history. The overall study helps in disease prevention and management of mycotoxins to ensure food safety.</p>
10acomparative genomics10aFusarium verticillioides10amycotoxin biosynthesis10aplant-pathogen interaction10aSecretome10awhole-genome sequencing1 aNavale, Vishwambar, D.1 aSawant, Amol, M.1 aGowda, Varun, U.1 aVamkudoth, Koteswara, Rao  uhttps://library.ncl.res.in/content/assembly-annotation-and-comparative-whole-genome-sequence-fusarium-verticillioides-isolated02468nas a2200229   4500008004100000022001400041245009600055210006900151260000800220300001400228490000700242520169800249653001401947653001601961653002901977653001402006653001802020100002702038700002102065700003002086856012202116       2023                        eng d  a1415-475700aGenetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India0 aGenetic diversity of toxigenic Fusarium verticillioides associat  cMAY  ae202200730 v463 a<p>In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F. coffeatum, F. foetens, and F. euwallaceae, 6.55%, 3.27%, and 1.63%, respectively. We isolated 54 strains of F. verticillioides, and their genetic diversity was studied by inter simple sequence repeats (ISSR). The ISSR fingerprints (AG) 8C and (AG) 8G showed 252 and 368 microsatellite sites in the genome of F. verticillioides and resulted in 99-100% repeatability and reproducibility. The Simpson (SID) and Shannon (H) indices (0.78 and 2.36) suggest that F. verticillioides strains exhibit moderate to high diversity. Molecular detection of fumonisin B1 (FB1) biosynthetic genes (FUM1 and FUM13) involved in FB1 production in F. verticillioides was confirmed by polymerase chain reaction (PCR). Furthermore, 91% of the strains were positive for FB1 production, which was affirmed by liquid chromatography with tandem mass spectrometry (LC-MS-MS). In-vitro appurtenance of F. verticillioides spores exhibited a high to moderate effect on the growth and development of the maize. The current finding demonstrated that most F. verticillioides strains showed a wide range of genetic diversity with varied toxigenic and pathogenic potentials. In conclusion, for the first time, F. coffeatum, F. foetens, and F. euwallaceae species were reported from maize grain samples in India. They were positive for FB1 and negatively affecting grain quality, which is a major concern in food safety.</p>
10adiversity10aFood safety10aFusarium verticillioides10aMycotoxin10apathogenicity1 aNavale, Vishwambar, D.1 aSawant, Amol, M.1 aVamkudoth, Koteswara, Rao  uhttps://library.ncl.res.in/content/genetic-diversity-toxigenic-fusarium-verticillioides-associated-maize-grains-india