02270nas a2200241 4500008004100000245007000041210006900111260000900180300001600189490000700205520148900212100002201701700002301723700002401746700001601770700002001786700002801806700002501834700002301859700002101882700002301903856010201926 2020 eng d00aApproach to nigericin derivatives and their therapeutic potential0 aApproach to nigericin derivatives and their therapeutic potentia cNOV  a43085-430910 v103 a

A new nigericin analogue that has been chemically modified was synthesized through a fluorination process from the parent nigericin, produced from a novel Streptomyces strain DASNCL-29. Fermentation strategies were designed for the optimised production of nigericin molecule and subjected for purification and structural analysis. The fermentation process resulted in the highest yield of nigericin (33% (w/w)). Initially, nigericin produced from the strain DASNCL-29 demonstrated polymorphism in its crystal structure, i.e., monoclinic and orthorhombic crystal lattices when crystallised with methanol and hexane, respectively. Furthermore, nigericin produced has been subjected to chemical modification by fluorination to enhance its efficacy. Two fluorinated analogues revealed that they possess a very potent antibacterial activity against Gram positive and Gram negative bacteria. To date, the nigericin molecule has not been reported for any reaction against Gram-negative bacteria, which are increasingly becoming resistant to antibiotics. For the first time, fluorinated analogues of nigericin have shown promising activity. In vitro cytotoxicity analysis of fluorinated analogues demonstrated tenfold lesser toxicity than the parent nigericin. This is the first type of study where the fluorinated analogues of nigericin showed very encouraging activity against Gram-negative organisms; moreover, they can be used as a candidate for treating many serious infections.

1 aSahu, Amit, Kumar1 aSaid, Madhukar, S.1 aHingamire, Tejashri1 aGaur, Megha1 aKhan, Abujunaid1 aShanmugam, Dhanasekaran1 aBarvkar, Vitthal, T.1 aDharne, Mahesh, S.1 aBharde, Atul, A.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/approach-nigericin-derivatives-and-their-therapeutic-potential01410nas a2200241 4500008004100000022001400041245009500055210006900150260000800219520058000227653002700807653002500834653002000859100002300879700002000902700002200922700002000944700001800964700002300982700001901005700002101024856012301045 2020 eng d a1478-641900aTotal synthesis of (-)-2-methoxy-2-butenolide-3-cinnamate and its antimicrobial potentials0 aTotal synthesis of 2methoxy2butenolide3cinnamate and its antimic cJUL3 a

The first total synthesis of (-)-2-methoxy-2-butenolide-3-cinnamate (butenolide cinnamate) was achieved using commercially available starting material. The synthesized compound was found to have promising antibacterial activity against Gram-negative strainsEscherichia coli(ATCC 8739),Salmonella typhimurium(ATCC 23564) andPseudomonas aeruginosa(ATCC 19154) with a minimum inhibitory concentration of 2.0 mu g/mL, 1.0 mu g/mL and 2.0 mu g/mL, respectively. Notably, the compound was more potent against Gram-negative test strains than the Gram-positive test strains.

10aantibacterial activity10abutenolide cinnamate10aTotal synthesis1 aSaid, Madhukar, S.1 aUdavant, Rohini1 aSahu, Amit, Kumar1 aKhan, Abujunaid1 aNayak, Rashmi1 aDastager, Syed, G.1 aKumar, Pradeep1 aGajbhiye, Jayant uhttps://library.ncl.res.in/content/total-synthesis-2-methoxy-2-butenolide-3-cinnamate-and-its-antimicrobial-potentials01352nas a2200217 4500008004100000022001400041245013700055210006900192260000800261300001400269490000700283520056800290100002000858700002300878700002600901700002000927700002100947700002200968700002300990856012101013 2022 eng d a0163-386400aEnceleamycins A-C, furo-naphthoquinones from amycolatopsis sp. MCC0218: isolation, structure elucidation, and antimicrobial activity0 aEnceleamycins AC furonaphthoquinones from amycolatopsis sp MCC02 cMAY a1267-12730 v853 a

Three novel furo-naphthoquinones, enceleamycins A-C (1-3), and a new N-hydroxypyrazinone acid (4) were identified from the strain Amycolatopsis sp. MCC 0218, isolated from a soil sample collected from the Western Ghats of India. Their chemical structure and absolute and relative configurations were established by 1D and 2D NMR spectroscopy, single-crystal X-ray crystallography, and high-resolution mass spectrometry. Compounds 1 and 3 were active against methicillin-susceptible and -resistant Staphylococcus aureus with MIC values of 2-16 mu g/mL.

1 aKhan, Abujunaid1 aSaid, Madhukar, S.1 aBorade, Balasaheb, R.1 aGonnade, Rajesh1 aBarvkar, Vitthal1 aKontham, Ravindar1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/enceleamycins-c-furo-naphthoquinones-amycolatopsis-sp-mcc0218-isolation-structure02106nas a2200217 4500008004100000245007300041210006900114260000900183300001600192490000700208520144800215653001001663653001101673653001601684653002001700653001001720100002001730700001601750700002301766856009901789 2023 eng d00aIn vitro anticancer evaluation of enceleamycin A and its underlying 0 aIn vitro anticancer evaluation of enceleamycin A and its underly cNOV  a34183-341930 v133 a

It has become more crucial than ever to find novel anticancer compounds due to the rise in cancer mortality and resistance to the present chemotherapeutic drugs. Naphthoquinones are regarded as privileged structures for their ability to inhibit various cancers. The current study examined three novel furo-naphthoquinones (Enceleamycins A-C) previously isolated from Amycolatopsis sp. MCC 0218 for their anticancer potential. Enceleamycin A demonstrated considerable cytotoxicity for triple-negative breast cancer (TNBC) MDA-MB-231 cells with an IC50 value of 1.25 mu g mL(-1) (3.78 mu M). It also showed the ability to inhibit MDA-MB-231 cell migration. Enceleamycin A raises intracellular ROS levels in TNBC cells, ultimately leading to apoptotic cell death, as demonstrated by Annexin V/PI staining. The molecular docking and simulation investigation revealed better binding affinity of Enceleamycin A with AKT2, which plays a vital role in breast cancer's invasiveness and chemo-resistance. Enceleamycin A inhibits the AKT2 enzyme in vitro with an IC50 value of 0.736 mu g mL(-1) (2.22 mu M), further validating the docking study. The in silico physicochemical and pharmacokinetics characteristics of Enceleamycin A demonstrated its drug-likeness. Intriguingly, Enceleamycin A is non-hemolytic in nature. Taken together, Enceleamycin A could be a candidate molecule for treating TNBC cells by targeting the AKT2 signaling pathway.

10aAssay10acancer10aDerivatives10aNaphthoquinones10aPlays1 aKhan, Abujunaid1 aPradeep, S.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/vitro-anticancer-evaluation-enceleamycin-and-its-underlying02427nas a2200277 4500008004100000022001400041245012800055210007000183260000900253490000700262520147600269653003801745653001001783653001801793653001701811653000901828100002201837700001801859700003001877700002401907700002001931700002001951700001801971700003001989856013002019 2024 eng d a0947-653900aBoron- and oxygen-doped π-extended helical nanographene with circularly polarised thermally activated delayed fluorescence0 aBoron and oxygendoped πextended helical nanographene with circul cAPR 0 v303 a

Helical nanographenes have garnered substantial attention owing to their finely adjustable optical and semiconducting properties. The strategic integration of both helicity and heteroatoms into the nanographene structure, facilitated by a boron-oxygen-based multiple resonance (MR) thermally activated delayed fluorescence (TADF), elevates its photophysical and chiroptical features. This signifies the introduction of an elegant category of helical nanographene that combines optical (TADF) and chiroptical (CPL) features. In this direction, we report the synthesis, optical, and chiroptical properties of boron, oxygen-doped pi-extended helical nanographene. The pi-extension induces distortion in the DOBNA-incorporated nanographene, endowing a pair of helicenes, (P)-B2NG, and (M)-B2NG exhibiting circularly polarized luminescence with glum of -2.3x10-3 and +2.5x10-3, respectively. B2NG exhibited MR-TADF with a lifetime below 5 mu s, and a reasonably high fluorescence quantum yield (50 %). Our molecular design enriches the optical and chiroptical properties of nanographenes and opens up new opportunities in multidisciplinary fields. B, O-doped pi-extended helical nanographene has been explored for its optical and chiroptical properties. The presence of helical and nanographene segments stabilize the helicity and imparts tunable optical properties. Helical nanographene display circularly polarized thermally activated delayed fluorescence. image

10acircularly polarized luminescence10aDOBNA10adual emission10ananographene10aTADF1 aVenugopal, Geethu1 aKumar, Viksit1 aJadhav, Ashok, Badrinaray1 aDongre, Sangram, D.1 aKhan, Abujunaid1 aGonnade, Rajesh1 aKumar, Jatish1 aBabu, Sukumaran, Santhosh uhttps://library.ncl.res.in/content/boron-and-oxygen-doped-%CF%80-extended-helical-nanographene-circularly-polarised-thermally04290nas a2200253 4500008004100000245013100041210006900172260000800241490000700249520336900256100002203625700002803647700002003675700002403695700003003719700002003749700001803769700002603787700002303813700001303836700002803849700002703877856013203904 2024 eng d00aIntegrated genomic and functional analysis of Streptomyces sp. UP1A-1 for bacterial wilt control and solanaceae yield increase0 aIntegrated genomic and functional analysis of Streptomyces sp UP cDEC0 v373 a

Ralstonia solanacearum is one of the most destructive soil-borne pathogen, causing bacterial wilt to the solanaceae vegetables. Streptomyces sp. UP1A-1 isolated from healthy solanaceae rhizosphere soil, exhibited the lowest disease incidence and increased fruit yield of solanaceae vegetables. However, the genomic and functional properties of UP1A-1 are unclear. Therefore, we conducted the present study to elucidate the genomic characteristics of UP1A-1 by whole genome sequencing. The results indicate that the genome of Streptomyces sp. UP1A-1 consists of 8,252,902 bp and contains 72.42 % G + C. We identified the genes that confer plant growth promoting (PGP) function, which include those involved in siderophore production, indole-3-acetic acid biosynthesis, phosphate solubilization, nitrogen metabolism, and potassium metabolism. We also identified several other genes, such as chitinase, peroxidase, superoxide dismutase, catalase, proline biosynthesis, and glucose dehydrogenase, which are believed to be involved in the control of wilt disease. These genes revealed that the strain UP1A-1 has physiologically adapted to varied environmental conditions and could potentially control both abiotic and biotic stresses.

1 aKaari, Manigundan1 aManikkam, Radhakrishnan1 aJoseph, Jerrine1 aKrishnan, Sakthivel1 aAnnamalai, Kishore, Kumar1 aKhan, Abujunaid1 aRajput, Vinay1 aDastager, Syed, Gulam1 aDharne, Mahesh, S.1 aUmar, Md1 aVenugopal, Gopikrishnan1 aAlexander, Balamurugan uhttps://library.ncl.res.in/content/integrated-genomic-and-functional-analysis-streptomyces-sp-up1a-1-bacterial-wilt-control-and