01670nas a2200205 4500008004100000022001400041245009800055210006900153260000800222300001600230490000700246520095900253100002201212700001501234700002101249700002101270700001701291700002601308856013001334 2019 eng d a0743-746300aSilk fibroin-sodium dodecyl sulfate gelation: molecular, structural, and rheological insights0 aSilk fibroinsodium dodecyl sulfate gelation molecular structural cNOV a14870-148780 v353 a

A gelling agent is necessary to accelerate sol to gel transition in an aqueous solution of silk fibroin (SF), which otherwise takes several days to complete. In this paper, we investigate the mechanism of gelation of Bornbyx mori SF by a model anionic surfactant, sodium dodecyl sulfate (SOS). Even though interactions between SDS and proteins have been extensively investigated, most of these studies have focused on globular proteins, which undergo denaturation. The interaction with a fibrous protein such as SF is different and results in an altered secondary structure leading to gelation. In this work, the concentration-dependent gelation process of the SF-SDS system is examined using rheology, SANS, FTIR, and NMR. We observed preferential binding of SDS to specific regions on the SF chain, which aids structural changes favoring beta-sheet formation. We propose a mechanism for the accelerated sol-gel transition in the SF-SDS system.

1 aHirlekar, Swarali1 aRay, Debes1 aAswal, Vinod, K.1 aPrabhune, Asmita1 aNisal, Anuya1 aRavindranathan, Sapna uhttps://library.ncl.res.in/content/silk-fibroin-sodium-dodecyl-sulfate-gelation-molecular-structural-and-rheological-insights01752nas a2200193 4500008004100000022001400041245007200055210006900127260000800196300001600204490000600220520113300226100002201359700001501381700002101396700002501417700001701442856009901459 2020 eng d a2470-134300aLauric acid sophorolipid: accelerating the gelation of silk fibroin0 aLauric acid sophorolipid accelerating the gelation of silk fibro cNOV a28571-285780 v53 a

Silk fibroin (SF) hydrogels find wide applications in tissue engineering. However, their scope has been limited due to the long gelation time in ambient conditions. This paper shows the reduction in gelation time of silk fibroin to minutes upon doping with a newly synthesized lauric acid sophorolipid (LASL). LASL comprises a fatty acid, lauric acid (with a 12-carbon aliphatic chain), that is derivatized by glucose molecules using a non-pathogenic yeast Candida bombicola. LASL was characterized using spectroscopic (Fourier transform infrared spectroscopy) and chromatographic (high-performance liquid chromatography, thin-layer chromatography, and high-resolution mass spectrometry) methods. This gelation of SF is comparable to the effect of an anionic surfactant, sodium dodecyl sulfate (SDS). The microstructure of SF-LASL hydrogels was investigated by small-angle neutron scattering (SANS) measurements and exhibited the beads-on-a-necklace model. The rheological properties of these hydrogels show similarity to SF-SDS hydrogels, therefore presenting a greener alternative for tissue engineering applications.

1 aHirlekar, Swarali1 aRay, Debes1 aAswal, Vinod, K.1 aPrabhune, Asmita, A.1 aNisal, Anuya uhttps://library.ncl.res.in/content/lauric-acid-sophorolipid-accelerating-gelation-silk-fibroin02458nas a2200277 4500008004100000020001300041245007900054210006900133260000800202300000900210490000700219520159500226653002501821653002001846653001401866653003001880653002601910653002601936100002201962700002001984700001802004700002202022700002102044700001702065856009802082 2021 eng d a0971119800aGreen antibacterial molecules: sophorolipids with varying fatty acid chain0 aGreen antibacterial molecules sophorolipids with varying fatty a cNOV a431+0 v353 aAntibiotic resistance is a rising problem of over the last decade making it difficult to treat infections caused by various pathogenic bacteria. To tackle this problem, newer antimicrobial strategies are being explored. Sophorolipids (SLs) are a class of biomolecules produced by a non-pathogenic yeast Starmarella bombicola (S. bombicold) with surfactant as well as potent broad spectrum antibacterial activity. In this work, we have successfully synthesized sophorolipids using different fatty acids with increasing chain length of hydrophobic tail; Lauric acid (C12)-LASL, Myristic acid (C14)-MASL, Palmitic acid (C16)-PASL and Stearic acid (C18)-SASL. We have used various techniques like FTIR, HRMS to characterize the synthesized SL. Further, we have measured properties like the critical micellar concentration for these biosurfactants and the SL's showed CMC less than or equal to 100 mg/L indicating excellent surface-active property. We have studied the antibacterial activity against gram-negative and gram-positive bacteria. Interestingly, the antibacterial activity was found to be more potent as the chain length of the fatty acid in the SL decreased. This trend was found to be reverse for toxicity towards mammalian cells. The shorter chain fatty acid SL's were found to be less cytotoxic. These results indicate that the sophorolipid candidates with shorter chain lengths would be beneficial for various biomedical applications such as tissue engineering, regenerative medicine and wound healing. Keywords: sophorolipid, biosurfactant, short chain fatty acid, antibacterial10aAntibacterial agents10aCells (Biology)10aInfection10aMicrobial drug resistance10aSaturated fatty acids10aSurface active agents1 aHirlekar, Swarali1 aAbhyankar, Isha1 aKane, Kartiki1 aTrimukhe, Kalpana1 aPrabhune, Asmita1 aNisal, Anuya uhttps://link.gale.com/apps/doc/A684660702/AONE?u=anon~d0cd1b4e&sid=googleScholar&xid=1a6d61d902246nas a2200181 4500008004100000022001400041245007300055210006900128260000800197300001100205490000700216520166400223100002001887700002201907700002101929700001701950856009701967 2024 eng d a1359-029400aBridging the gap: an investigation of biosurfactants-polymer systems0 aBridging the gap an investigation of biosurfactantspolymer syste cAUG a1018060 v723 a

Biosurfactants (BSs) have been extensively researched due to their potential applications in various fields, including textiles, cosmetics, pharmaceuticals, agriculture, and oil remediation. These BSs possess a diverse range of physical, chemical, and biological properties. In recent years, researchers have combined these biosurfactants with both natural and synthetic polymers, resulting in the development of advanced material systems that exhibit a unique combination of properties. This review focuses on highlighting the recent advancements in these biosurfactant-polymer material systems and identifies existing gaps in the literature. The combination of biosurfactants with polymers has led to the formation of interpenetrated hydrogels, films, chemically modified surfaces, vesicles, functionalized nanofiber nonwoven mats, nano-formulations, and nano-assemblies. Some studies have also investigated the interactions between biosurfactants and polymer molecules. In most cases, non-specific, non-covalent interactions, such as electrostatic interactions, hydrogen bonding, and hydrophobic interactions have been found to govern the properties of these systems. Moreover, promising results have been achieved through the covalent modification of polymer surfaces, followed by functionalization using biosurfactant molecules. The literature demonstrates that these advanced materials could find applications in various fields, including drug delivery, bioremediation, biomedical materials, and as antimicrobial agents. These findings indicate the promising potential of biosurfactant-polymer systems for future advancements in these areas.

1 aAbhyankar, Isha1 aHirlekar, Swarali1 aPrabhune, Asmita1 aNisal, Anuya uhttps://library.ncl.res.in/content/bridging-gap-investigation-biosurfactants-polymer-systems