00739nas a2200157   4500008004100000022001400041245014700055210006900202260009500271300000800366490000800374100002200382700002500404700002400429856012800453       2012                        eng d  a0006-291X00aTrehalose as an indicator of desiccation stress in Drosophila melanogaster larvae: a potential marker of anhydrobiosis (vol 419, pg 638, 2012)0 aTrehalose as an indicator of desiccation stress in Drosophila me  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcMAY  a8600 v4211 aThorat, Leena, J.1 aGaikwad, Sushama, M.1 aNath, Bimalendu, B.  uhttps://library.ncl.res.in/content/trehalose-indicator-desiccation-stress-drosophila-melanogaster-larvae-potential-marker-102563nas a2200241   4500008004100000022001400041245012300055210006900178260009500247300001200342490000800354520164900362653001802011653001402029653002102043653001602064653002802080653001402108100002202122700002502144700002402169856012802193       2012                        eng d  a0006-291X00aTrehalose as an indicator of desiccation stress in Drosophila melanogaster larvae: a potential marker of anhydrobiosis0 aTrehalose as an indicator of desiccation stress in Drosophila me  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcMAR  a638-6420 v4193 a<p>In the current scenario of global climate change, desiccation is considered as one of the major environmental stressors for the biota exposed to altered levels of ambient temperature and humidity. Drosophila melanogaster, a cosmopolitan terrestrial insect has been chosen as a humidity-sensitive bioindicator model for the present study since its habitat undergoes frequent stochastic and/or seasonally aggravated dehydration regimes. We report here for the first time the occurrence of anhydrobiosis in D. melanogaster larvae by subjecting them to desiccation stress under laboratory conditions. Larvae desiccated for ten hours at &lt;5% relative humidity could enter anhydrobiosis and could revive upon rehydration followed by resumption of active metabolism. As revealed by FIR and HPLC analyzes, our findings strongly indicated the synthesis and accumulation of trehalose in the desiccating larvae. Biochemical measurements pointed out the desiccation-responsive trehalose metabolic pathway that was found to be coordinated in concert with the enzymes trehalose 6-phosphate synthase and trehalase. Further, an inhibitor-based experimental approach using deoxynojirimycin, a specific trehalase inhibitor, demonstrated the pivotal role of trehalose in larval anhydrobiosis of D. melanogaster. We therefore propose trehalose as a potential marker for the assessment of anhydrobiosis in Drosophila. The present findings thus add to the growing list of novel biochemical markers in specific bioindicator organisms for fulfilling the urgent need of environmental biomonitoring of climate change. (C) 2012 Elsevier Inc. All rights reserved.</p>10aAnhydrobiosis10abiomarker10aDeoxynojirimycin10aDesiccation10aDrosophila melanogaster10atrehalose1 aThorat, Leena, J.1 aGaikwad, Sushama, M.1 aNath, Bimalendu, B.  uhttps://library.ncl.res.in/content/trehalose-indicator-desiccation-stress-drosophila-melanogaster-larvae-potential-marker-202108nas a2200241   4500008004100000022001400041245007600055210006900131260008100200300001200281490000700293520126000300653002801560653002601588653002401614653001401638653001401652100001701666700002201683700002401705700002501729856011201754       2015                        eng d  a0959-665800aInsect trehalase: physiological significance and potential applications0 aInsect trehalase physiological significance and potential applic  aJOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USAbOXFORD UNIV PRESS INCcAPR  a357-3670 v253 a<p>Trehalose, a non-reducing disaccharide, is widespread throughout the biological world. It is the major blood sugar in insects playing a crucial role as an instant source of energy and in dealing with abiotic stresses. The hydrolysis of trehalose is under the enzymatic control of trehalase. The enzyme trehalase is gaining interest in insect physiology as it regulates energy metabolism and glucose generation via trehalose catabolism. The two forms of insect trehalase namely, Tre-1 and Tre-2, are important in energy supply, growth, metamorphosis, stress recovery, chitin synthesis and insect flight. Insect trehalase has not been reviewed in depth and the information available is quite scattered. The present mini review discusses our recent understanding of the regulation, mechanism and biochemical characterization of insect trehalase with respect to its physiological role in vital life functions. We also highlight the molecular and biochemical properties of insect trehalase that makes it amenable to competitive inhibition by most glycosidase inhibitors. Due to its crucial role in carbon metabolism in insects, application of inhibitors against trehalose can form a promising area towards formulating strategies for insect pest control.</p>10acarbohydrate metabolism10aglycosidase inhibitor10aglycoside hydrolase10atrehalase10atrehalose1 aShukla, Ekta1 aThorat, Leena, J.1 aNath, Bimalendu, B.1 aGaikwad, Sushama, M.  uhttps://library.ncl.res.in/content/insect-trehalase-physiological-significance-and-potential-applications-0