02399nas a2200145 4500008004100000245013100041210006900172260000800241520180000249100002002049700002002069700001902089700002202108856012302130 2019 eng d00aDevelopment of low-cost plant probiotic formulations of functional endophytes for sustainable cultivation of Coleus forskohlii0 aDevelopment of lowcost plant probiotic formulations of functiona cAUG3 a

Deployment of plant endophytes at field level is reported to make an impact on agricultural crop productivity; development and deployment of suitable crop specific plant probiotics in a suitable delivery matrix is a value-added task. In our study, we attempted to develop bioformulations of native, fungal endophytes of Coleus forskohlii to improve plant yield using two different carrier-based materials (talc and wheat bran). Initially, fungal endophytes (RF1, SF1, and SF2) were grown on sterilized wheat bran under solid state condition and their growth kinetics and pattern were analyzed by ergosterol content and scanning electron microscope, respectively. 10-day-grown fungal endophytic cultures were used for the development of two types of formulations (wheat bran and talc-based formulations) and tested for their efficacy on host plant, C. forskohlii under field conditions. Interestingly, application of wheat bran-based endophytic formulations significantly (p < 0.01) enhanced plant height (12-29%), number of branches (51-63%), root biomass (26-33%), photosynthetic pigments (32-101%), and forskolin content (35-56%) compared to talc-based formulations under field conditions. Shelf life of endophytes (RF1, SF1, and SF2) in both formulations revealed spore viability in wheat bran-based formulations for 6 months storage period as compared to talc-based formulations. Overall, the present investigation envisages developing plant probiotic bioformulations of functional endophytes of C. forskohlii to enhance root biomass and in planta forskolin content.

1 aMastan, Anthati1 aRane, Digeshwar1 aDastager, Syed1 aVivekBabu, C., S. uhttps://library.ncl.res.in/content/development-low-cost-plant-probiotic-formulations-functional-endophytes-sustainable02503nas a2200265 4500008004100000022001400041245013300055210006900188260000800257300001200265490000700277520158600284653002501870653001301895653001601908653001101924653002201935653001401957653004001971100002002011700002002031700002302051700004002074856012302114 2020 eng d a1867-130600aPlant probiotic bacterial endophyte, alcaligenes faecalis, modulates plant growth and forskolin biosynthesis incoleus forskohlii0 aPlant probiotic bacterial endophyte alcaligenes faecalis modulat cJUN a481-4930 v123 a

Coleus forskohliiis an herb, well-known for its medicinal compound forskolin present in its roots, with wide range of pharmaceutical applications. Here, we report, for the first time, the role of plant-probiotic bacterial endophytes ofC. forskohlii, CFLB1 and CFRB1, isolated from leaf and root, which regulate plant growth andin plantforskolin content. Native bacterial endophyte, CFRB1 (Alcaligenes faecalis), significantly modulates primary plant productivity and forskolin content under pot and field conditions. Under field conditions, CFRB1 endophyte application significantly enhanced photosynthetic pigments and reduced the severity of root-knot and root rot diseases. Expression analyses of functional genes involved in the forskolin biosynthesis inC. forskohliiplants treated with CFRB1 endophyte under field conditions revealed differential upregulation of fourC. forskohliiditerpene synthases (CfTPSs),CfTPS1,CfTPS2,CfTPS3andCfTPS4, along with cytochrome P450 (CfCYP76AH15) and acyltransferase (CfACT1-8) genes. CFRB1 treatment reduced the severity of nematode infection and root rot inC. forskohliiplants by 81 and 78%, respectively. Overall, we demonstrate that cross-talk of plant-endophyte interaction inC. forskohliiis beneficial, leading to enhanced forskolin content through modulation of forskolin biosynthetic pathway genes along with increased plant yield and reduced disease incidence. Thus, endophytic isolate,A. faecalis(CFRB1), could be deployed as a novel bio-stimulant for enhancingin plantaforskolin content during cultivation ofC. forskohlii.

10aAlcaligenes faecalis10aCfACT1-810aCfCYP76AH1510aCfTPSs10aColeus forskohlii10aForskolin10aPlant-probiotic bacterial endophyte1 aMastan, Anthati1 aRane, Digeshwar1 aDastager, Syed, G.1 aBabu, Chikkarasanahalli, Shivegowda uhttps://library.ncl.res.in/content/plant-probiotic-bacterial-endophyte-alcaligenes-faecalis-modulates-plant-growth-and02221nas a2200253 4500008004100000022001400041245015000055210006900205260000800274300001100282490000800293520134100301653001901642653002201661653001501683653001401698653001401712653002301726100002001749700002001769700002301789700002301812856013201835 2021 eng d a0031-942200aMolecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii0 aMolecular insights of fungal endophyte coinoculation with Tricho cAPR a1126540 v1843 a

To understand the compatibility of three native endophytic fungi Phialemoniopsis cornearis (SF1), Macrophomina pseudophaseolina (SF2) and Fusarium redolens (RF1) with Trichoderma viride (TV1) on Coleus forskohlii in enhancing plant growth and forskolin content, field experiments were conducted. Co-inoculation of RF1+TV1 showed significant improvement in plant growth (52%), root biomass (67%), and in planter forskolin content (94%), followed by treatment with SF2+TV1 and SF1+TV1. qRT-PCR was carried out to quantify expression of five key forskolin biosynthetic pathway genes (CfTPS2, CfTPS3, CfTPS4, CfCYP76AH15, and CfACT1-8) in RF1+TV1 treated C. forskohlii plants. Elevated expression of CfTPS2, CfTPS4, CfCYP76AH15 and CfACT1-8 genes was observed with RF1+TV1 combination as compared to uninoculated C. forskohlii plants. Besides, RF1+TV1 treatment considerably reduced the severity of nematode infection of C. forskohlii plants under field conditions. Thus, congruent properties of F. redolens (RF1) were witnessed with co-inoculation of T. viride (TV1) under field conditions which resulted in enhanced forskolin content, root biomass, and reduced nematode infections in C. forskohlii. Overall, this approach could be an economical and sustainable step towards cultivation of commercially important medicinal plants.

10aCo-inoculation10aColeus forskohlii10aendophytes10aForskolin10aLamiaceae10aTrichoderma viride1 aMastan, Anthati1 aRane, Digeshwar1 aDastager, Syed, G.1 aBabu, C., S. Vivek uhttps://library.ncl.res.in/content/molecular-insights-fungal-endophyte-co-inoculation-trichoderma-viride-augmentation-forskolin