03578nas a2200181 4500008004100000245012100041210006900162260000800231300001400239490000700253520288500260100002503145700000503170700003103175700002703206700003303233856013003266 2019 eng d00aImmobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles0 aImmobilization studies of Candida Antarctica lipase B on gallic  cMAY a3235-32440 v143 a

Purpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell.

Methods: Using a convenient co-precipitation method, we prepared Fe3O4 nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid-formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption.

Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins Also, optimal immobilization temperature and pH were 38 degrees C and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.

1 aSreeHarsha, Nagaraja1 a1 aAlzahrani, Abdullah, Mossa1 aAl-Dhubiab, Bandar, E.1 aVenugopala, Katharigatta, N. uhttps://library.ncl.res.in/content/immobilization-studies-candida-antarctica-lipase-b-gallic-acid-resin-grafted-magnetic-iron02492nas a2200277 4500008004100000022001400041245016500055210006900220260000800289300001100297490000800308520149600316653001801812653001201830653001801842653002701860100002101887700002701908700002301935700002501958700002701983700002302010700003102033700002602064856012402090 2020 eng d a1381-514800aEpoxy functionalized polymer grafted magnetic nanoparticles by facile surface initiated polymerization for immobilization studies of Candida Antarctica lipase B0 aEpoxy functionalized polymer grafted magnetic nanoparticles by f cFEB a1044540 v1473 a

Immobilization of Candida Antarctica lipase B (Cal-B) was done on the epoxy functionalized polymer (EFP) grafted magnetic nanoparticles (MNPs) via covalent attachment with the active epoxy groups. The EFP brushes were grafted on iron-oxide based MNPs by a facile surface-initiated atom transfer radical polymerization (ATRP) using activators generated by electron transfer (AGET) of glycidyl methacrylate (GMA). Each step of the surface modification, polymer grafting, and enzyme immobilization process on the polymer grafted MNPs was studied using Fourier transform infrared spectroscopy (FTIR). A thermogravimetric analysis (TGA) calculated the amount of engineered organic components, a transmission electron microscopy (TEM) visualized the core-shell formation of the MNPs, and a vibrating sample magnetometer (VSM) validated their magnetic properties at various modification stages. The lipase immobilization efficiency was described as a function of immobilization time, as well as, enzyme amount. The activity was characterized within a range of pH, temperature, kinetic parameters, resusability and storage stability, for both the free and immoblized Cal-B enzyme. The results of this study suggested that poly(GMA) grafted MNPs can be successfully used for the immobilization of Cal-B with improved efficiencies compared to those obtained with free soluble lipase. The reported enzyme immobilization method appears to be reproducible and scalable for industrial production.

10aCal-B enzymes10aenzymes10aImmoblization10aMagnetic nanoparticles1 aAfzal, Haq, Asif1 aGhorpade, Ravindra, V.1 aThorve, Asmita, K.1 aNagaraja, Sreeharsha1 aAl-Dhubiab, Bandar, E.1 aMeravanige, Girish1 aRasool, Sahibzada, Tasleem1 aRoopashree, Teeka, S. uhttps://library.ncl.res.in/content/epoxy-functionalized-polymer-grafted-magnetic-nanoparticles-facile-surface-initiated