03578nas a2200181 4500008004100000245012100041210006900162260000800231300001400239490000700253520288500260100002503145700000503170700003103175700002703206700003303233856013003266 2019 eng d00aImmobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles0 aImmobilization studies of Candida Antarctica lipase B on gallic  cMAY a3235-32440 v143 a

Purpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell.

Methods: Using a convenient co-precipitation method, we prepared Fe3O4 nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid-formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption.

Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins Also, optimal immobilization temperature and pH were 38 degrees C and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.

1 aSreeHarsha, Nagaraja1 a1 aAlzahrani, Abdullah, Mossa1 aAl-Dhubiab, Bandar, E.1 aVenugopala, Katharigatta, N. uhttps://library.ncl.res.in/content/immobilization-studies-candida-antarctica-lipase-b-gallic-acid-resin-grafted-magnetic-iron