00665nas a2200169   4500008004100000245012900041210006900170260000800239300001200247490000700259100002300266700001700289700001900306700002100325700002000346856012900366       2006                        eng d00aEfficient protocol for genetic transformation and shoot regeneration of turmeric (Curcuma longa L.) via particle bombardment0 aEfficient protocol for genetic transformation and shoot regenera  cMAR  a112-1160 v251 aShirgurkar, M., V.1 aNaik, V., B.1 avon Arnold, S.1 aNadgauda, R., S.1 aClapham, D., H.  uhttps://library.ncl.res.in/content/efficient-protocol-genetic-transformation-and-shoot-regeneration-turmeric-curcuma-longa-l01557nas a2200217   4500008004100000022001400041245012600055210006900181260011700250300001000367490000600377520065400383653002701037653001801064653001701082653003701099653002001136100002301156700003301179856012701212       2010                        eng d  a0973-626300aStudies of antimicrobial activity of turmeric (Curcuma Longa L.) leaves and rhizomes collected during plant growth phases0 aStudies of antimicrobial activity of turmeric Curcuma Longa L le  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcMAY  a24-280 v53 a<p>Turmeric is the processed underground rhizome used as spice, herbal medicines, dying agent and cosmetics. The leaves and rhizomes of this plant were collected at periodic intervals. These samples were air dried, powdered and extracted with MeOH and EtOH. Antibacterial activity of these extracts was determined against three different index microbes, gram-positive cocci, gram-negative rod and cocci using well diffusion method with appropriate controls. Antimicrobial activity of the 4-month-old leaves was found to be the maximum while that of mature and fresh rhizomes was the maximum against both gram-positive and Gram-negative bacteria.</p>10aantibacterial activity10aCurcuma longa10aLeaf extract10aMinimum inhibitory concentration10arhizome extract1 aShirgurkar, M., V.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/studies-antimicrobial-activity-turmeric-curcuma-longa-l-leaves-and-rhizomes-collected-002140nas a2200205   4500008004100000022001400041245008000055210006900135260005700204300001200261490000700273520142200280100001601702700002701718700001601745700000901761700002301770700002701793856011401820       2015                        eng d  a0971-589400aEfficient shoots regeneration and genetic transformation of bacopa monniera0 aEfficient shoots regeneration and genetic transformation of baco  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcAPR  a261-2670 v213 a<p>Bacopa monniera is an important source of metabolites with pharmaceutical value. It has been regarded as a valuable medicinal plant and its entire commercial requirement is met from wild natural population. Recently, metabolic engineering has emerged as an important solution for sustained supply of assured and quality raw material for the production of active principles. Present report describes efficient in vitro multiplication and transformation method for geneticmanipulation of this species. MS medium supplemented with 2 mgl(-1) BA and 0.2 mgl(-1) IAA was found optimum for maximum shoot regeneration (98.33 %) from in vitro leaves with 2-3 longitudinal cuts. Agrobacterium tumefaciens-mediated transformation method was used for generating transgenic B. monniera plants. Putative transformants were confirmed by GUS assay and PCR based confirmation of hptII gene. DNA blot analysis showed single copy insertion of transgene cassette. An average of 87.5 % of the regenerated shoots were found PCR positive for hptII gene and GUS activity was detected in leaves of transgenic shoots at a frequency of 82.5 % The efficient multiple shoots regeneration system described herein may help in mass production of B. monniera plant. Also, the high frequency transformation protocol described here can be used for genetic engineering of B. monniera for enhancement of its pharmaceutically important metabolites.</p>1 aKumari, Uma1 aVishwakarma, Rishi, K.1 aGupta, Neha1 aRuby1 aShirgurkar, M., V.1 aKhan, Bashir, Mohammad  uhttps://library.ncl.res.in/content/efficient-shoots-regeneration-and-genetic-transformation-bacopa-monniera-0