02574nas a2200253   4500008004100000022001400041245013200055210006900187260000800256300001200264490000700276520169200283653002601975653002502001653002502026653001802051653001202069653002902081100002002110700001802130700002002148700002402168856012802192       2019                        eng d  a1438-793X00aTranscriptomics analysis of propiconazole-treated Cochliobolus sativus reveals new putative azole targets in the plant pathogen0 aTranscriptomics analysis of propiconazoletreated Cochliobolus sa  cMAY  a453-4650 v193 a<p>Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a filamentous fungus from the class Dothideomycetes. It is a pathogen of cereals including wheat and barley, and causes foliar spot blotch, root rot, black point on grains, head blight, leaf blight, and seedling blight diseases. Annual yields of these economically important cereals are severely reduced due to this pathogen attack. Evolution of fungicide resistant pathogen strains, availability of a limited number of potent antifungal compounds, and their efficacy are the acute issues in field management of phytopathogenic fungi. Propiconazole is a widely used azole fungicide to control the disease in fields. The known targets of azoles are the demethylase enzymes involved in ergosterol biosynthesis. Nonetheless, azoles have multiple modes of action, some of which have not been explored yet. Identifying the off-target effects of fungicides by dissecting gene expression profiles in response to them can provide insights into their modes of action and possible mechanisms of fungicide resistance. Moreover it can also reveal additional targets for development of new fungicides. Hence, we analyzed the global gene expression profile of C. sativus on exposure to sub-lethal doses of propiconazole in a time series. The gene expression patterns were confirmed using quantitative reverse transcriptase PCR (qRT-PCR). This study revealed overexpression of target genes from the sterol biosynthesis pathway supporting the reported mode of resistance against azoles. In addition, some new potential targets have also been identified, which could be explored to develop new fungicides and plant protection strategies.</p>
10aBipolaris sorokiniana10aCochliobolus sativus10aFungicide resistance10aPropiconazole10aRNA-seq10aTranscriptomics analysis1 aSomani, Deepika1 aAdhav, Ragini1 aPrashant, Ramya1 aKadoo, Narendra, Y.  uhttps://library.ncl.res.in/content/transcriptomics-analysis-propiconazole-treated-cochliobolus-sativus-reveals-new-putative02482nas a2200277   4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520152400261653001801785653003101803653001301834653002601847653002001873100002301893700002401916700002001940700002701960700002901987700003302016700002402049856013102073       2025                        eng d  a0971-589400aTranscriptome analyses reveal TaWRKY41 as a potential candidate governing spot blotch resistance in wheat0 aTranscriptome analyses reveal TaWRKY41 as a potential candidate   cAPR  a591-6080 v313 a<p>
	Spot blotch disease caused by Bipolaris sorokiniana poses a significant threat to wheat production. Cultivation of disease-resistant wheat genotypes appears to be the most practical approach to mitigate the impact of this devastating disease. However, the molecular responses of wheat plants during spot blotch disease progression remain poorly understood. This study employed RNA-sequencing to unravel the spatiotemporal molecular events underlying the resistance mechanism in the spot blotch susceptible and resistant wheat genotypes. This study further provides a comprehensive overview of differentially expressed transcripts through functional analysis and transcription factor identification, elucidating the biological mechanisms governing wheat-B. sorokiniana interaction. In the resistant genotype, the expression of one of the key transcription factors, TaWRKY41, was significantly induced upon pathogen inoculation. Computational studies, electrophoretic-mobility shift assay, and yeast one-hybrid assay confirmed the interaction of the recombinant TaWRKY41 protein with W-box elements present in the promoters of plant defense-related genes. Furthermore, co-expression network analyses identified downstream genes positively correlated with TaWRKY41, providing insights into their probable involvement in the defense response. Overall, our investigation suggests that TaWRKY41 contributes to spot blotch resistance in wheat. This knowledge can help develop new disease-resistant wheat varieties.</p>
10aPlant defense10aplant-pathogen interaction10aTaWRKY4110aTranscription factors10aTranscriptomics1 aDayama, Bhakti, R.1 aMahadik, Varsha, A.1 aSomani, Deepika1 aShinde, Balkrishna, A.1 aKondhare, Kirtikumar, R.1 aKarthikeyan, Muthukumarasamy1 aKadoo, Narendra, Y.  uhttps://library.ncl.res.in/content/transcriptome-analyses-reveal-tawrky41-potential-candidate-governing-spot-blotch-resistance