03284nas a2200289 4500008004100000022001400041245012400055210006900179260000800248300001400256490000700270520233000277653001302607653001902620653001602639653002002655653001902675653001302694100002502707700002602732700002102758700002302779700002602802700001802828700002802846856012002874 2019 eng d a0885-328200aClopidogrel eluting electrospun polyurethane/polyethylene glycol thromboresistant, hemocompatible nanofibrous scaffolds0 aClopidogrel eluting electrospun polyurethanepolyethylene glycol  cMAY a1327-13470 v333 a

Biomaterials used as blood-contacting material must be hemocompatible and exhibit lower thrombotic potential while maintaining hemostasis and angiogenesis. With the aim of developing thromboresistant, hemocompatible nanofibrous scaffolds, polyurethane/polyethylene glycol scaffolds incorporated with 1, 5, and 10 wt% Clopidogrel were fabricated and evaluated for their physiochemical properties, biocompatibility, hemocompatibility, and antithrombotic potential. The results of physicochemical characterization revealed the fabrication of nanometer-sized scaffolds with smooth surfaces. The incorporation of both polyethylene glycol and Clopidogrel to polyurethane enhanced the hydrophilicity and water uptake potential of polyurethane/polyethylene glycol/Clopidogrel scaffolds. The dynamic mechanical analysis revealed the enhancement in mechanical strength of the polyurethane/polyethylene glycol scaffolds on incorporation of Clopidogrel. The polyurethane/polyethylene glycol/Clopidogrel scaffolds showed a tri-phasic drug release pattern. The results of hemocompatibility assessment demonstrated the excellent blood compatibility of the polyurethane/polyethylene glycol/Clopidogrel scaffolds, with the developed scaffolds exhibiting lower hemolysis, increased albumin and plasma protein adsorption while reduction in fibrinogen adsorption. Further, the platelet adhesion was highly suppressed and significant increase in coagulation period was observed for Clopidogrel incorporated scaffolds. The results of cell adhesion and cell viability substantiate the biocompatibility of the developed nanofibrous scaffolds with the HUVEC cell viability on polyurethane/polyethylene glycol, polyurethane/polyethylene glycol/Clopidogrel-1, 5, and 10% at day 7 found to be 12.35, 13.36, 14.85, and 4.18% higher as compared to polyurethane scaffolds, and the NIH/3T3 cell viability found to be 35.27, 70.82, 36.60, and 7.95% higher as compared to polyurethane scaffolds, respectively. Altogether the results of the study advocate the incorporation of Clopidogrel to the polyurethane/polyethylene glycol blend in order to fabricate scaffolds with appropriate antithrombotic property, hemocompatibility, and cell proliferation capacity and thus, might be successfully used as antithrombotic material for biomedical application.

10aadhesion10acardiovascular10acoagulation10aelectrospinning10aHemocompatible10aplatelet1 aShitole, Ajinkya, A.1 aGiram, Prabhanjan, S.1 aRaut, Piyush, W.1 aRade, Priyanka, P.1 aKhandwekar, Anand, P.1 aSharma, Neeti1 aGarnaik, Baijayantimala uhttps://library.ncl.res.in/content/clopidogrel-eluting-electrospun-polyurethanepolyethylene-glycol-thromboresistant02679nas a2200181 4500008004100000245007500041210006900116260000800185300001600193490000600209520204900215653003802264653001902302653001902321100002302340700002802363856010602391 2020 eng d00aOfloxacin-loaded PLLA nanofibrous mats for wound dressing applications0 aOfloxacinloaded PLLA nanofibrous mats for wound dressing applica cAUG a6648–66600 v33 a

Poly(l-lactide) (PLLA) was synthesized from l-lactide in the presence of a zinc salen complex. Ofloxacin (OFLX)-loaded PLLA nanofibrous mats were fabricated by electrospinning using dichloromethane/dimethyl sulfoxide (4:1 v/v) solutions containing different amounts of OFLX (1, 3, 5, and 10 w/w%). The morphology and diameter of nanofibrous mats were studied by scanning electron microscopy (SEM). The internal morphology of the nanofibers was examined by transmission electron microscopy (TEM). Miscibility between OFLX and PLLA in nanofibrous mats was confirmed by attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopy. Differential scanning calorimetry (DSC) confirmed the interaction of OFLX with PLLA nanofibrous mats. The presence of OFLX in PLLA nanofibrous mats increased the tensile strength significantly, which was confirmed by dynamic mechanical analysis (DMA). The in vitro drug release profile was studied at pH values 4.6, 5.8, and 7.4. OFLX-loaded PLLA nanofibrous mats showed burst release till first 12 h, and sustained release followed up to 168 h. The in vitro biocompatibility test of nanofibrous mats was carried out using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against the NIH/3T3 fibroblast cell line. The results of MTT assay demonstrated cell viability and cell proliferation at a concentration as low as 10 w/w%, which proved their nontoxicity and biocompatibility. OFLX-loaded PLLA nanofibrous mats exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli. The in vivo wound healing study on rats showed 44, 65, and 88% wound closure rates on 14th day with control, PLLA, and OFLX-loaded PLLA nanofibrous mats, respectively. The results proved that the PLLA nanofibrous mats loaded with an appropriate concentration of OFLX are promising candidates for wound dressing applications.

10ananofibers nontoxic biocompatible10aPLLA ofloxacin10awound dressing1 aRade, Priyanka, P.1 aGarnaik, Baijayantimala uhttps://library.ncl.res.in/content/ofloxacin-loaded-plla-nanofibrous-mats-wound-dressing-applications01460nas a2200217 4500008004100000022001400041245009900055210006900154260000900223300001200232490000700244520071700251653001800968653002800986653001301014653000901027653002801036100002301064700002801087856012701115 2020 eng d a1023-666X00aSynthesis and characterization of biocompatible poly (L-lactide) using zinc (II) salen complex0 aSynthesis and characterization of biocompatible poly Llactide us cJUL  a283-2990 v253 a

A biocompatible zinc (II) complex based on a tetradentate N,N,O,O-type salen ligand was synthesized, characterized and used for the solvent-free ring-opening polymerization (ROP) of L-lactide in bulk at 180 degrees C to prepare high molecular weight poly(L-lactide) (M-n: 82,600 Da;M-w: 140,000 Da; PDI: 1.70). Poly(L-lactide) (PLLA) was characterized using FTIR,H-1 NMR,C-13 NMR, GPC, TGA, DSC, WAXD, and MALDI-ToF. Kinetic measurement was carried out and first-order behavior to monomer was observed. Thek(app)was found as 6 +/- 0.001 x 10(-4 )s(-1). The biocompatibility of the PLLA was confirmed byin vitrocytotoxicity against NIH/3T3 fibroblast cell line and can be used in biomedical applications.

10aBiocompatible10aBiomedical Applications10ain vitro10aPLLA10azinc (II) salen complex1 aRade, Priyanka, P.1 aGarnaik, Baijayantimala uhttps://library.ncl.res.in/content/synthesis-and-characterization-biocompatible-poly-l-lactide-using-zinc-ii-salen-complex02370nas a2200253 4500008004100000022001400041245019400055210006900249260000800318300001200326490000600338520140500344100002601749700002501775700002201800700002301822700002101845700001801866700002201884700002401906700002801930700002601958856013201984 2021 eng d a2047-483000aGreen synthesis of methoxy-poly(ethylene glycol)-block-poly(l-lactide-co-glycolide) copolymer using zinc proline as a biocompatible initiator for irinotecan delivery to colon cancer in vivo0 aGreen synthesis of methoxypolyethylene glycolblockpolyllactideco cFEB a795-8060 v93 a

Poly(lactic-co-glycolic acid) (PLGA) is the most commonly described biocompatible copolymer used in biomedical applications. In this work, a green synthetic approach based on the biocompatible zinc proline complex, as an initiator for PLGA synthesis, is reported for the first time for the synthesis of methoxy-poly(ethylene glycol)-block-poly(l-lactic-co-glycolic acid) (mPEG-PLGA). mPEG-PLGA with controlled molecular weight and narrow polydispersity was synthesised. Its potential for delivery of irinotecan (Ir), a poorly water-soluble chemotherapeutic drug used for the treatment of colon and pancreatic cancer, was studied. Nanoparticles of controlled size (140-160 nm), surface charge (similar to-10 mV), release properties and cytotoxicity against CT-26 (colon) and BxPC-3 (pancreatic) cancer cells, were prepared. Tumor accumulation was confirmed by optical imaging of fluorescently labelled nanoparticles. Unlike Tween (R) 80 coated NP-Ir, the Pluronic (R) F-127 coated NP-Ir exhibits significant tumor growth delay compared to untreated and blank formulation treated groups in the CT-26 subcutaneous tumor model, after 4 treatments of 30 mg irinotecan per kg dose. Overall, this proof-of-concept study demonstrates that the newly synthesized copolymer, via a green route, is proven to be nontoxic, requires fewer purification steps and has potential applications in drug delivery.

1 aGiram, Prabhanjan, S.1 aWang, Julie, Tzu-Wen1 aWalters, Adam, A.1 aRade, Priyanka, P.1 aAkhtar, Muhammad1 aHan, Shunping1 aFaruqu, Farid, N.1 aAbdel-Bar, Hend, M.1 aGarnaik, Baijayantimala1 aAl-Jamal, Khuloud, T. uhttps://library.ncl.res.in/content/green-synthesis-methoxy-polyethylene-glycol-block-polyl-lactide-co-glycolide-copolymer-using01709nas a2200193 4500008004100000020001400041245014700055210006900202260000800271300001000279490000600289520097400295100002301269700002601292700002501318700001801343700002801361856012601389 2021 eng d a2524-793X00aPhysicochemical and in vitro antibacterial evaluation of metronidazole loaded eudragit S-100 nanofibrous mats for the intestinal drug delivery0 aPhysicochemical and in vitro antibacterial evaluation of metroni cAUG a76-880 v43 a

Metronidazole (MTZ) loaded Eudragit S-100 (ES-100) nonwoven nanofibrous mats were successfully electrospun and evaluated for intestinal drug delivery. MTZ was varied in the range of 1–15% (w/w) in ES-100 nanofibrous mats, the morphological characterization of nanofibrous mats was carried out using FE-SEM and the average diameter of nanofiber was found in the range 150–600 nm. WAXD and DSC demonstrated the amorphous nature of MTZ in ES-100 nanofibrous mats. Their contact angle analysis confirmed the hydrophobic nature. The mechanical strength of ES-100 nanofibrous mats decreased with increasing MTZ concentration. The drug release profiles showed 74% MTZ release from ES-100d within 2 h at pH 6.8 which is the colonic environment. Antibacterial activities against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli) showed that the ES-100 nanofibrous mats loaded with MTZ exhibited good activity.

1 aRade, Priyanka, P.1 aGiram, Prabhanjan, S.1 aShitole, Ajinkya, A.1 aSharma, Neeti1 aGarnaik, Baijayantimala uhttps://library.ncl.res.in/content/physicochemical-and-vitro-antibacterial-evaluation-metronidazole-loaded-eudragit-s-100