01283nas a2200217 4500008004100000022001400041245013200055210006900187260000900256300001400265490000700279520052100286100001800807700001800825700001900843700002000862700001900882700001600901700001700917856013100934 2019 eng d a1463-926200aRapid and efficient sequestration of arsenic from contaminated water using hypertolerant Bacillus L-148 sp.: a two-step process0 aRapid and efficient sequestration of arsenic from contaminated w cMAY  a2245-22510 v213 a

A fast, robust and green bioreactor for arsenic sequestration from contaminated water was designed. In the two-tier bioreactor, bacterial arsenite detoxification (1 mM) was carried out followed by precipitation of arsenate in 5 min. The precipitate could be used for supercapacitor applications. This bioreactor yielded arsenic free water, therefore, this method can be adopted for scale-up. The combination of hypertolerant bacteria and fast precipitation indicates the robustness of this pilot bioreactor.

1 aBagade, Aditi1 aNandre, Vinod1 aGhosh, Sayanti1 aBattu, Shateesh1 aHaram, Santosh1 aGiri, Ashok1 aKodam, Kisan uhttps://library.ncl.res.in/content/rapid-and-efficient-sequestration-arsenic-contaminated-water-using-hypertolerant-bacillus-l02265nas a2200277 4500008004100000022001400041245008300055210006900138260000800207300001100215490000800226520139500234653002801629653001501657653002201672653002701694653003101721100001801752700001801770700001701788700002001805700001801825700001601843700001701859856011101876 2020 eng d a0269-749100aCharacterisation of hyper tolerant Bacillus firmus L-148 for arsenic oxidation0 aCharacterisation of hyper tolerant Bacillus firmus L148 for arse cJUN a1141240 v2613 a

Groundwater arsenic pollution causes millions of deaths worldwide. Long term natural and anthropogenic activities have increased arsenic levels in groundwater causing higher threats of arsenic exposure. Arsenic hyper-tolerant Firmicute Bacillus firmus L-148 was isolated from arsenic limiting Lonar lake soil, which tolerated more than 3 M arsenic and could oxidize 75 mM arsenite [As(III)] in 14 days. It oxidized As(III) in presence of heavy metals and had unusual pH optima at 9.2. B. firmus L-148 was studied at the biochemical, protein, genomic and transcript level for understanding its arsenic oxidizing machinery. The proteomic and transcript analysis exhibited the presence of ars and aio operon and supported the inducible nature of ars operon. Robust, hyper-tolerant, fast As(III) oxidizing, least nutrient requiring and multi-metal resistance qualities of the strain were used in microcosm studies for bioremediation. Artificial groundwater mimicking microcosm with 75 mM As(III) was developed. Modulation of carbon source, iron and multi metals affected growth and As(III) oxidation rate. The As(III) oxidation was recorded to be 77% in 15 days in presence of sodium acetate and Fe ions. This microcosm study can be explored for bioremediation of arsenic contaminated water and followed by precipitation using other methods. (C) 2020 Elsevier Ltd. All rights reserved.

10aArsenic hyper-tolerance10aLonar lake10aMicrocosm studies10aMulti-metal resistance10aNext generation sequencing1 aBagade, Aditi1 aNandre, Vinod1 aPaul, Dhiraj1 aPatil, Yugendra1 aSharma, Nisha1 aGiri, Ashok1 aKodam, Kisan uhttps://library.ncl.res.in/content/characterisation-hyper-tolerant-bacillus-firmus-l-148-arsenic-oxidation02128nas a2200241 4500008004100000022001400041245014400055210006900199260000800268300001100276490000800287520128400295653002501579653002201604653002301626653001501649653001701664100001901681700001901700700001901719700001701738856013101755 2024 eng d a0304-389400aHarnessing the potential of Achromobacter sp. M1 to remediate heavy metals from wastewater: genomic insights and environmental applications0 aHarnessing the potential of Achromobacter sp M1 to remediate hea cDEC a1361250 v4803 a

Lead, mercury, and cadmium are classified as toxic under the toxic Substances' Priority List by CDC-ATSDR (Center for Disease Control-Agency for Toxic Substances and Disease Registry). This toxic trio is capable of disrupting the one-health harmony due to its human, animal, and environmental hazards. The present study aimed in removing the toxic trio within 24 h using a novel Achromobacter sp. M1. Atomic absorption spectroscopic evaluation for removal efficiency of Pb, Hg, and Cd by M1 was 68.8 +/- 0.9%, 82.7 +/- 1.9%, and 94.9 +/- 1.2 %, respectively, within 24 h. Lab-scale evaluation of strain M1 with wastewater showed the removal of the toxic trio together with the reduction in TSS from 140 to 118 ppm, BOD from 100 to 58 ppm, and COD from 381 to 222 ppm. Furthermore, strain M1 was capable of mitigating heavy metal stress and promoting plant growth, evidenced through chlorophyll, malondialdehyde, and proline estimation, together with the production of indole acetic acid (23.84 mu g/mL), siderophore (85 %), and solubilization of silica (39.66 mu g/mL). Whole genome sequencing revealed an ANI of 89 %, indicating a novel species of Achromobacter genus. A total of 23 putative genes for Cd, Hg, and Pb resistance were identified through genome mining.

10aAchromobacter sp. M110aMetal biosorption10aMetal transporters10aToxic trio10aWhole genome1 aMahale, Mithil1 aSamson, Rachel1 aDharne, Mahesh1 aKodam, Kisan uhttps://library.ncl.res.in/content/harnessing-potential-achromobacter-sp-m1-remediate-heavy-metals-wastewater-genomic-insights