01559nas a2200217   4500008004100000022001400041245011500055210006900170260000800239300001400247490000700261520076500268100002701033700003001060700001601090700002701106700002301133700002801156700002501184856013201209       2019                        eng d  a1477-052000aDemystifying a hexuronic acid ligand that recognizes Toxoplasma gondii and blocks its invasion into host cells0 aDemystifying a hexuronic acid ligand that recognizes Toxoplasma   cMAY  a4535-45420 v173 a<p>Toxoplasma gondii is a ubiquitous eukaryotic pathogen responsible for toxoplasmosis in humans and animals. This parasite is an obligate intracellular pathogen and actively invades susceptible host cells, a process which is mediated by specific receptor-ligand interactions. Here, we have identified an unnatural 2,4-disulfated D-glucuronic acid (Di-S-GlcA), a hexuronic acid composed of heparin/ heparan sulfate, as a potential carbohydrate ligand that can selectively bind to T. gondii parasites. More importantly, the gelatin conjugated Di-S-GlcA multivalent probe displayed strong inhibition of parasite entry into host cells. These results open perspective for the future use of Di-S-GlcA epitopes in biomedical applications against toxoplasmosis.</p>
1 aSubramani, Balamurugan1 aShantamurthy, Chethan, D.1 aMaru, Parag1 aBelekar, Meenakshi, A.1 aMardhekar, Sandhya1 aShanmugam, Dhanasekaran1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/demystifying-hexuronic-acid-ligand-recognizes-toxoplasma-gondii-and-blocks-its-invasion-host02262nas a2200217   4500008004100000022001400041245011000055210006900165260000900234300001500243490000700258520148900265100002801754700002601782700002101808700001601829700002801845700002401873700001901897856012801916       2020                        eng d  a0305-104800aTDR Targets 6: driving drug discovery for human pathogens through intensive chemogenomic data integration0 aTDR Targets 6 driving drug discovery for human pathogens through  cJAN   aD992-D10050 v483 a<p>The volume of biological, chemical and functional data deposited in the public domain is growing rapidly, thanks to next generation sequencing and highly-automated screening technologies. These datasets represent invaluable resources for drug discovery, particularly for less studied neglected disease pathogens. To leverage these datasets, smart and intensive data integration is required to guide computational inferences across diverse organisms. The TDR Targets chemogenomics resource integrates genomic data from human pathogens and model organisms along with information on bioactive compounds and their annotated activities. This report highlights the latest updates on the available data and functionality in TDR Targets 6. Based on chemogenomic network models providing links between inhibitors and targets, the database now incorporates network-driven target prioritizations, and novel visualizations of network subgraphs displaying chemical- and target-similarity neighborhoods along with associated target-compound bioactivity links. Available data can be browsed and queried through a new user interface, that allow users to perform prioritizations of protein targets and chemical inhibitors. As such, TDR Targets now facilitates the investigation of drug repurposing against pathogen targets, which can potentially help in identifying candidate targets for bioactive compounds with previously unknown targets. TDR Targets is available at https://tdrtargets.org.</p>
1 aLandaburu, Lionel, Uran1 aBerenstein, Ariel, J.1 aVidela, Santiago1 aMaru, Parag1 aShanmugam, Dhanasekaran1 aChernomoretz, Ariel1 aAguero, Fernan  uhttps://library.ncl.res.in/content/tdr-targets-6-driving-drug-discovery-human-pathogens-through-intensive-chemogenomic-data