02089nas a2200241   4500008004100000022001400041245015400055210006900209260000800278300000800286490000600294520125200300653000901552653001201561653001201573653002701585653003401612100002301646700001601669700001601685700002201701856012401723       2019                        eng d  a2190-572X00aUse of Plackett-Burman design for enhanced phytase production by Williopsis saturnus NCIM 3298 for applications in animal feed and ethanol production0 aUse of PlackettBurman design for enhanced phytase production by   cJUN  a2370 v93 a<p>Distiller-dried grain solid (DDGS), a co-product of alcohol production, contains cereal grain residues, proteins, and yeast metabolites, which make it suitable in poultry feeding. However, high phytate content of DDGS limits its applicability in poultry feed. In this study, Plackett-Burman design was used to improve cell-bound phytase production by Williopsis saturnus NCIM 3298, and we achieved an enzyme activity of 269IU/g of dry-wet biomass. The effect of this enhanced phytase-displaying yeast strain on hydrolysis of corn phytate and subsequently on ethanol production and DDGS quality was then investigated. Results of saccharification in the presence of phytase showed that reducing sugar content of liquefied mash increased by 11%, which subsequently improved the ethanol production by 18% (w/v) (p&lt;0.01) compared with the control. Notably, phytase treatment decreased the phytate content of corn by 70% (p&lt;0.01) compared with the control, thereby improving the availability of free phosphate in fermentation broth and DDGS. Thus, the results obtained suggest that the addition of W. saturnus NCIM 3298 strain has the potential of providing a new source of phytase that would be useful in the feed and ethanol industries.</p>
10aDDGS10aEthanol10aPhytase10aPlackett-Burman Design10aWilliopsis saturnus NCIM 32981 aPable, Anupama, A.1 aShah, Sarah1 aKumar, Ravi1 aKhire, Jayant, M.  uhttps://library.ncl.res.in/content/use-plackett-burman-design-enhanced-phytase-production-williopsis-saturnus-ncim-329802131nas a2200265   4500008004100000022001400041245011800055210006900173260000800242300001000250490000800260520122700268653002501495653001701520653002101537653002701558653001601585653001001601100002501611700002301636700002501659700002301684700002701707856013101734       2021                        eng d  a0168-165600aRhizobacterial consortium mediated aroma and yield enhancement in basmati and non-basmati rice ( Oryza sativa L.)0 aRhizobacterial consortium mediated aroma and yield enhancement i  cFEB  a47-580 v3283 a<p>Basmati and non-basmati rice varieties are commercially important. Aromatic rice varieties are low yielding and recently depletion in aroma is observed due to the shift towards modern agriculture. Therefore, it is necessary to restore the aroma and increase the yield through sustainable agriculture. The use of microbial bioinoculants is one of the promising ways to achieve these targets. With these objectives, rhizospheric bacterial strains Enterobacter hormaechei (AM122) and Lysinibacillus xylanilyticus (DB25) having the property of synthesizing 2acetyl-1-pyrroline (2AP) were isolated from the rhizosphere of two aromatic rice varieties, Ambemohar-157 and Dehradun Basmati respectively and their effect on plant growth, aroma and yield enhancement under mono-inoculation and consortium conditions was analyzed. The bacterial inoculum in consortium resulted in significant improvement in vegetative growth, yield and 2AP content over mono inoculation and control. The study highlights the potential of E. hormaechei and L. xylanilyticus in plant growth, yield and aroma enhancement in basmati and non-basmati rice varieties. These strains can be taken up further for developing a commercial bioformulation.</p>
10a2-Acetyl-1-pyrroline10aBasmati rice10aNon-basmati rice10aplant growth promotion10aRhizosphere10ayield1 aDhondge, Harshal, V.1 aPable, Anupama, A.1 aBarvkar, Vitthal, T.1 aDastager, Syed, G.1 aNadaf, Altafhusain, B.  uhttps://library.ncl.res.in/content/rhizobacterial-consortium-mediated-aroma-and-yield-enhancement-basmati-and-non-basmati-rice02726nas a2200277   4500008004100000022001400041245011000055210006900165260000800234300001100242490000800253520182900261653001502090653002402105653001502129653001702144653001602161653000902177100002102186700002502207700001702232700002302249700002302272700002702295856012602322       2022                        eng d  a0944-501300aExploring the core microbiota in scented rice (Oryza sativa L.) rhizosphere through metagenomics approach0 aExploring the core microbiota in scented rice Oryza sativa L rhi  cOCT  a1271570 v2633 a<p>
	Rice is a major food crop cultivated around the globe. Specially scented rice varieties are of commercial importance but they are low-yielding. The rhizospheric microflora plays a significant role in improving yield and aroma. However, the core microbiome of the scented rice rhizosphere is comparatively less explored. Here, we analyzed the core microbiome associated with the rhizosphere of the scented (Ambemohar-157 and Dehradun basmati) in comparison with non-scented rice (Kolam and Arize 6444 Gold) cultivated at two different geoclimatic zones of India (Maharashtra and Uttarakhand) using the metagenomics approach. The alpha and beta diversity analysis showed that the microbial communities associated with scented and non-scented varieties significantly changes with respect to richness, diversity, and evenness. The taxonomic profiling revealed the variation in composition, diversity, and abundance of the microbiome in terms of phyla and genera associated with scented rice varieties over non-scented. The cluster analysis distinguishes the microbial communities based on their geographical positions. The core microbiome analysis revealed that scented rice rhizosphere shelters distinct and unique microbiota. 28.6 % of genera were exclusively present only in the scented rice rhizosphere. The putative functional gene annotation revealed the high abundance of genes related to the biosynthesis of 2-acetyl-1-pyrroline (2AP) precursors in scented rice. The precursor feeding analysis revealed proline as a preferred substrate by 2AP synthesizing bacteria. The 2AP precursor proline and proline metabolism genes showed a positive correlation. The scented rice-specific rhizobacteria pointed out in this study can be used as bioinoculants for enhancing aroma, yield, and sustainable rice cultivation.</p>
10aMetagenome10aMicrobial community10aMicrobiome10aOryza sativa10aRhizosphere10arice1 aDhondge, Harshal1 aBarvkar, Vitthal, T.1 aPaul, Dhiraj1 aDastager, Syed, G.1 aPable, Anupama, A.1 aNadaf, Altafhusain, B.  uhttps://library.ncl.res.in/content/exploring-core-microbiota-scented-rice-oryza-sativa-l-rhizosphere-through-metagenomics02549nas a2200241   4500008004100000022001400041245009700055210006900152260000800221300000800229490000700237520175400244100001501998700002102013700001802034700002302052700001902075700002502094700001902119700002502138700002302163856012102186       2023                        eng d  a0343-865100aBacterial communities and diversity of western ghats soil: a study of a biodiversity hotspot0 aBacterial communities and diversity of western ghats soil a stud  cAPR  a1080 v803 a<p>
	The Western Ghats is one of India's mega-diversity hotspots and an ecologically and geologically important area for the diversity of endemic plants and animals. The present study provides insights into the aerobic bacterial diversity and composition of the soils of North Western Ghats located in Maharashtra state (NWGM), India. The samples for the culture-dependent study were collected from 6 different locations namely Malshej Ghat, Bhimashankar, Lonavala, Mulshi, Tail-Baila, and Mahabaleshwar. A total of 173 isolates were obtained from the different samples, which belonged to Proteobacteria (43%), Firmicutes (36%), and Actinobacteria (19%). Sequences of 15 strains shared &lt;= 98.7% similarity (a species cut-off) which represent potential novel species. Metagenomic analysis revealed the presence of Actinobacteria and Proteobacteria as the most dominant phyla at both MB and MG. However, both sites showed variation in the composition of rare phyla and other dominant phyla. This difference in bacterial community composition could be due to differences in altitude or other physicochemical properties. The functional prediction from the amplicon sequencing showed the abundance of carbohydrate, protein, and lipid metabolism which was corroborated by screening the isolated bacterial strains for the same. The present study has a unique take on microbial diversity and defines the importance of community assembly processes such as drift, dispersal, and selection. Such processes are relatively important in controlling community diversity, distribution, as well as succession. This study has shown that the microbial community of NWGM is a rich source of polysaccharide degrading bacteria having biotechnological potential.</p>
1 aGhare, Uma1 aNarvekar, Simran1 aLodha, Tushar1 aMallebhari, Rubiya1 aDastager, Syed1 aBarvkar, Vitthal, T.1 aDhotre, Dhiraj1 aKarmalkar, Nitin, R.1 aPable, Anupama, A.  uhttps://library.ncl.res.in/content/bacterial-communities-and-diversity-western-ghats-soil-study-biodiversity-hotspot02902nas a2200301   4500008004100000022001400041245009800055210006900153260000900222300001100231490000800242520188100250653002502131653002402156653003702180653002702217653001802244653002602262100002502288700002502313700002302338700002302361700001802384700002302402700002202425700002702447856012602474       2024                        eng d  a0168-160500aGenome sequencing and protein modeling unraveled the 2AP biosynthesis in Bacillus cereus DB250 aGenome sequencing and protein modeling unraveled the 2AP biosynt  cMAR   a1106000 v4133 a<p>
	2-Acetyl-1-pyrroline (2AP) is an important and major flavor aroma compound responsible for the fragrance of basmati rice, cheese, wine, and several other food products. Biosynthesis of 2AP in aromatic rice and a few other plant species is associated with a recessive Betaine aldehyde dehydrogenase 2 (BADH2) gene. However, the literature is scant on the relationship between the functional BADH2 gene and 2AP biosynthesis in prokaryotic systems. Therefore, in the present study, we aimed to explore the functionality of the BADH2 gene for 2AP biosynthesis in 2AP synthesizing rice rhizobacterial isolate Bacillus cereus DB25 isolated from the rhizosphere of basmati rice (Oryza sativa L.). Full-length BcBADH2 sequence was obtained through whole genome sequencing (WGS) and further confirmed through traditional PCR and Sanger sequencing. Then the functionality of the BcBADH2 gene was evaluated in-silico through bioinformatics analysis and protein docking studies and further experimentally validated through enzyme assay. The sequencing and bioinformatics analysis results revealed a full-length 1485 bp BcBADH2 coding sequence without any deletion or premature stop codons. Full-length BcBADH2 was found to encode a fully functional protein of 54.08 kDa with pI of 5.22 and showed the presence of the conserved amino acids responsible for enzyme activity. The docking studies confirmed a good affinity between the protein and its substrate whereas the presence of BcBADH2 enzyme activity confirmed the functionality of BADH2 enzyme in B. cereus DB25. In conclusion, the findings of the present study suggest that B. cereus DB25 is able to synthesize 2AP despite a functional BADH2 gene and there may be a different molecular mechanism responsible for 2AP biosynthesis in bacterial systems, unlike that found in aromatic rice and other eukaryotic plant species.</p>
10a2-Acetyl-1-pyrroline10aBasmati rice flavor10aBetaine aldehyde dehydrogenase 210aProtein-ligand docking10aRhizobacteria10aWhole-genome sequence1 aDhondge, Harshal, V.1 aBarvkar, Vitthal, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S.1 aRajput, Vinay1 aPable, Anupama, A.1 aHenry, Robert, J.1 aNadaf, Altafhusain, B.  uhttps://library.ncl.res.in/content/genome-sequencing-and-protein-modeling-unraveled-2ap-biosynthesis-bacillus-cereus-db25