02636nas a2200265   4500008004100000022001400041245012500055210006900180260000900249300001200258490000800270520175800278653001702036653001602053653000802069653000802077653002702085653001702112100002102129700002202150700002802172700002002200700002402220856012602244       2019                        eng d  a0039-914000aQuantification of niclosamide polymorphic forms - a comparative study by Raman, NIR and MIR using chemometric techniques0 aQuantification of niclosamide polymorphic forms a comparative st  cJUL   a679-6880 v1993 a<p>Niclosamide, an anthelmintic drug recently repurposed for its activity against cancer, crystallizes into three solvated forms, two monohydrates (NHa, NHb) and one anhydrous (NAn) form. NAn is sensitive to pseudopolymorphic transformations that affect its dissolution and consequently, its bioavailability. NAn exhibits a polymorphic conversion to metastable monohydrate (NHa) form during high-energy milling in presence of poorly soluble solvents like water. It is hence very important to quantify polymorphic conversion from NAn to NHa, as water is a commonly used solvent during various processing like ball milling and wet granulation. This main objective of the study was to examine the feasibility of Raman, NIR and MIR spectroscopic techniques for identification and quantification of polymorphic forms of niclosamide in binary mixtures and multicomponent mixtures. Calibration models were developed and validated by vibrational spectroscopic techniques in binary mixtures of NAn and NHa and in multicomponent mixtures by chemometric techniques. These techniques were further used to identify and quantify NHa during ball milling, granulation and in presence of other polymorphic forms of niclosamide. Identification and quantification of pseudopolymorphs in binary and multicomponent mixtures with an acceptable recovery of 100.13-102.99% for Raman and 100.07-101.28% for NIR with low % RSD of 2.38-3.12 for both techniques were obtained. The % NHa determined during ball milling and granulation was similar by NIR and Raman. Raman spectroscopy however showed a greater advantage over other techniques in determining the NHa in presence of NHb due to significant difference in the spectral region of hydrates, when compared to NIR and MIR.</p>10aBall milling10aGranulation10aMSC10aPLS10aPolymorphic conversion10aspectroscopy1 aBhavana, Valmala1 aChavan, Rahul, B.1 aMannava, M., K. Chaitan1 aNangia, Ashwini1 aShastri, Nalini, R.  uhttps://library.ncl.res.in/content/quantification-niclosamide-polymorphic-forms-comparative-study-raman-nir-and-mir-using