02623nas a2200229   4500008004100000022001400041245012800055210006900183260000800252300001100260490000800271520184300279653001302122653001902135653001102154653002002165653001602185100002302201700001802224700001902242856013202261       2021                        eng d  a0013-935100aFunctional metagenomic landscape of polluted river reveals potential genes involved in degradation of xenobiotic pollutants0 aFunctional metagenomic landscape of polluted river reveals poten  cJAN  a1103320 v1923 a<p>Rapid industrialization contributes substantially to xenobiotic pollutants in rivers. As a result, most of the rivers traversing urban settlements are in significantly deteriorated conditions. These pollutants are recalcitrant, requiring robust catabolic machinery for their complete transformation into bioavailable and non-toxic byproducts. Microbes are versatile dwellers that could adapt to such contaminants by using them as a source of nutrients during growth. However, efficient bioremediation requires an in-depth knowledge of microbial diversity and their metabolism related genes in the polluted niches. We employed MinION shotgun sequencing, to comprehend the biodegradation related genes and their function potential operating in the polluted urban riverine system of Western India. A vast number of catabolic genes were detected for the xenobiotic pollutants such as Benzoate, Nitrotoluene, Aminobenzoate, Drug metabolism, and Polycyclic Aromatic Hydrocarbons. Aerobic, and anaerobic catabolism genes, were mapped for their ability of degradation of xenobiotics. Interestingly, catabolism profiles of multiple aromatic compounds culminated into the Benzoate degradation pathway, suggesting it as a plausible central pathway for the autochthonous bacterial communities. Further mapping with RemeDB database, predicted plastic and dye degrading enzymes. Moreover, the diversity indices for the pollutant degrading enzymes suggested little variations (R-2 value of 18%) between the city and non-city (outskirts of city limits) riverine stretch indicating the impact of industrialization in the outskirts of the city stretch as well. Altogether, this study would serve as a preliminary baseline for future explorations concerning river cleaning programs and also exploiting such microbes for bioremediation applications.</p>
10aBenzoate10aBioremediation10aMinION10aRiverine system10aXenobiotics1 aYadav, Rakeshkumar1 aRajput, Vinay1 aDharne, Mahesh  uhttps://library.ncl.res.in/content/functional-metagenomic-landscape-polluted-river-reveals-potential-genes-involved-degradation02557nas a2200217   4500008004100000022001400041245014200055210006900197260000800266300001600274490000700290520179300297653001902090653001702109653001102126653001902137653001502156100002302171700001902194856012602213       2024                        eng d  a0944-134400aUtility of metagenomics for bioremediation: a comprehensive review on bioremediation mechanisms and microbial dynamics of river ecosystem0 aUtility of metagenomics for bioremediation a comprehensive revie  cMAR  a18422-184340 v313 a<p>
	Global industrialization has contributed substantial amounts of chemical pollutants in rivers, resulting in an uninhabitable state and impacting different life forms. Moreover, water macrophytes, such as water hyacinths, are abundantly present in polluted rivers, significantly affecting the overall water biogeochemistry. Bioremediation involves utilizing microbial metabolic machinery and is one of the most viable approaches for removing toxic pollutants. Conventional techniques generate limited information on the indigenous microbial population and their xenobiotic metabolism, failing the bioremediation process. Metagenomics can overcome these limitations by providing in-depth details of microbial taxa and functionality-related information required for successful biostimulation and augmentation. An in-depth summary of the findings related to pollutant metabolizing genes and enzymes in rivers still needs to be collated. The present study details bioremediation genes and enzymes functionally mined from polluted river ecosystems worldwide using a metagenomic approach. Several studies reported a wide variety of pollutant-degrading enzymes involved in the metabolism of dyes, plastics, persistent organic pollutants, and aromatic hydrocarbons. Additionally, few studies also noted a shift in the microbiome of the rivers upon exposure to contaminants, crucially affecting the ecological determinant processes. Furthermore, minimal studies have focused on the role of water-hyacinth-associated microbes in the bioremediation potentials, suggesting the need for the bioprospecting of these lesser-studied microbes. Overall, our study summarizes the prospects and utilities of the metagenomic approach and proposes the need to employ it for efficient bioremediation.</p>
10aBioremediation10aMetagenomics10aRivers10aWater hyacinth10aXenobiotic1 aYadav, Rakeshkumar1 aDharne, Mahesh  uhttps://library.ncl.res.in/content/utility-metagenomics-bioremediation-comprehensive-review-bioremediation-mechanisms-and