01987nas a2200169   4500008004100000245007500041210006900116260000800185300001200193490000600205520142100211100001401632700001401646700001701660700002201677856011801699       2016                        eng d00aCetyl alcohol and oleic acid sophorolipids exhibit anticancer activity0 aCetyl alcohol and oleic acid sophorolipids exhibit anticancer ac  cFEB  a399-4020 v83 aObjective: Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to have anticancer activity. We investigated the anti-cancer activity of cetyl alcohol sophorolipids (CAS) and oleic acid sophorolipids (OAS) in breast cancer (MCF-7, MDA-MB-231), cervical cancer (SiHa, HeLa) and non-cancerous (HaCaT and RAW264.7) cell lines. Methods: For cell viability assay, MCF-7, MDA-MB-231, SiHa, HeLa, HaCaT and RAW264.7 cell lines were treated with different concentrations (0-160 μg/ml) of OAS and CAS for 24h. The cell viability was determined by MTT dye uptake method. Cell proliferation assay was determined by using trypan blue dye exclusion method. Results: Our preliminary data shows that compared to OAS, CAS exhibited more significant reduction in the viability of MCF-7, MDA-MB-231 and SiHa. However, compared to CAS, OAS induced more decrease in viability in HeLa cells. Interestingly, both the types of SLs did not affect the viability of non-cancerous cells. Moreover, CAS, when used as a coating material, induced proliferation in macrophage cell line, RAW264.7. Conclusion: The present study provides an important clue towards the anti-cancer potential of OAS and CAS derived from Candida bombicola. Interestingly, the ability of CAS to promote the proliferation of non-cancerous cells suggests its future application as a scaffold for enhancing the adhesion and proliferation of normal cells.1 aDubey, P.1 aRaina, P.1 aPrabhune, A.1 aKaul-Ghanekar, R.  uhttps://www.scopus.com/inward/record.url?eid=2-s2.0-84959564023&partnerID=40&md5=54a0f06b61b63c66744a7580e4e091d602416nas a2200301   4500008004100000020001400041022001400055245012000069210006900189260000800258300001600266490000700282520145200289653001501741653001201756653001901768653000601787653001901793653002201812100002401834700001601858700002401874700001701898700002401915700002301939700002201962856013001984       2020                        eng d  a1572-8862  a1040-727800aInhibition of quorum sensing, motility and biofilm formation ofpseudomonas aeruginosaby copper oxide nanostructures0 aInhibition of quorum sensing motility and biofilm formation ofps  cOCT  a1531 - 15410 v323 a<p>Quorum sensing (QS) is the communication between bacterial cells governed by their population density and regulated by the genes controlling virulence factors and biofilm formation. Multiple mechanisms of biofilms are resistive to antimicrobial chemotherapy; therefore novel strategies are required to overcome its limitations. Here, we report the effect of various copper oxide nanostructures (CuO-NSs) on quorum sensing inhibition. The two-dimensional CuO-NSs such as interlaced nanodiscs, nanodiscs and leaf-shaped nanosheets are prepared via a simple chemical method. The Quorum sensing inhibition (QSI) activity of all the CuO-NS are examined using reporter strainChromobacterium violaceumCV026 andEscherichia colipSB1142. We found that the CuO-interlaced nanodisc structures exhibit better QSI activity than nanodiscs and leaf-shaped sheets. The interlaced nanodisc structures are inhibited various long-chainN-acyl homoserine lactones (AHLs) mediated QS individually and confirmed by other QS-associated phenomena forPseudomonas aeruginosa, including biofilm inhibition, inhibition of virulence factors such as pyocyanin, protease production and swarming motility. Thus QSI activity of CuO-NSs is solely dependent on specific shape offering large surface area and more active sites. The CuO-NS is effective quorum sensing inhibitors, which has potential clinical applications in the management ofP. aeruginosaassociated infections.</p>
10aaeruginosa10aBiofilm10aNanostructures10aP10aquorum sensing10aVirulence factors1 aDesai, Dnyanada, G.1 aSwarali, H.1 aNavale, Govinda, R.1 aPrabhune, A.1 aLate, Dattatray, J.1 aDharne, Mahesh, S.1 aWalke, Pravin, S.  uhttps://library.ncl.res.in/content/inhibition-quorum-sensing-motility-and-biofilm-formation-ofpseudomonas-aeruginosaby-copper