02001nas a2200193   4500008004100000245014500041210006900186260000800255300001200263490000900275520127500284100001801559700002001577700002001597700002201617700001801639700002201657856012801679       2017                        eng d00aStability studies of cuticle degrading and mycolytic enzymes of myrothecium verrucaria for control of insect pests and fungal phytopathogens0 aStability studies of cuticle degrading and mycolytic enzymes of   cJUN  a404-4120 v16  3 aMyrothecium verrucaria produced extracellularly hydrolytic enzymes which can hydrolyse the insect cuticle as well as fungal cell wall. The addition of polyols, such as glycerol, sorbitol, xylitol (1 M) during ultra -filtration or freeze- drying of enzyme mixture increased the recovery of the enzymes in a concentrated form. Polyols (5 M) increased the temperature and pH stability of the enzymes, in the presence of glycerol chitinase, beta-1,3-glucanase, lipase and protease retained 50-60% of initial activities at 40 degrees C after 3 h. While xylitol (5 M) was effective in stabilizing activities at pH 5.0 and 7.5 at 25 degrees C for 7 d. In the freeze-dried powder form, > 90% at 4 degrees C for 1 year and 80-85% at 25 degrees C for 2 months enzyme activities were retained. The addition of glycerol (1 M) to the enzyme mixture protected enzyme activities under sunlight (60-65% activity at RT) for 5 d. Because of glycerol (1 M), the efficacy of M verrucaria enzyme preparation to control Helicoverpa armigera infestation in chick pea was increased to 70 +/- 19%. While without glycerol the efficacy was 55 +/- 23%. The germination of peanut seeds infected with Sclerotium rolfsii was observed to be increased (70 +/- 5%) in a pot irrigated with enzyme mixture.1 aChavan, S., B1 aVidhate, R., P.1 aKallure, G., S.1 aDandawate, N., L.1 aKhire, J., M.1 aDeshpande, M., V.  uhttps://library.ncl.res.in/content/stability-studies-cuticle-degrading-and-mycolytic-enzymes-myrothecium-verrucaria-control