02201nas a2200229   4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520141300261100001801674700002101692700001901713700001801732700002301750700002501773700002301798700002101821856012901842       2016                        eng d  a0894-028200aPyramiding PvPGIP2 and TAXI-III but not PvPGIP2 and PMEI enhances resistance against fusarium graminearum0 aPyramiding PvPGIP2 and TAXIIII but not PvPGIP2 and PMEI enhances  cAUG  a629-6390 v293 a<p>Plant protein inhibitors counteract the activity of cell wall degrading enzymes (CWDEs) secreted by pathogens to breach the plant cell-wall barrier. Transgenic plants expressing a single protein inhibitor restrict pathogen infections. However, since pathogens secrete a number of CWDEs at the onset of infection, we combined more inhibitors in a single wheat genotype to reinforce further the cell-wall barrier. We combined polygalacturonase (PG) inhibiting protein (PGIP) and pectin methyl esterase inhibitor (PMEI), both controlling the activity of PG, one of the first CWDEs secreted during infection. We also pyramided PGIP and TAXI-M, a xylanase inhibitor that controls the activity of xylanases, key factors for the degradation of xylan, a main component of cereal cell wall. We demonstrated that the pyramiding of PGIP and PMEI did not contribute to any further improvement of disease resistance. However, the presence of both pectinase inhibitors ensured a broader spectrum of disease resistance. Conversely, the PGIP and TAXI-HI combination contributed to further improvement of Fusarium head blight (FHB) resistance, probably because these inhibitors target the activity of different types of CWDEs, i.e., PGs and xylanases. Worth mentioning, the reduction of FHB symptoms is accompanied by a reduction of deoxynivalenol accumulation with a foreseen great benefit to human and animal health.</p>1 aTundo, Silvio1 aKalunke, Raviraj1 aJanni, Michela1 aVolpi, Chiara1 aLionetti, Vincenzo1 aBellincampi, Daniela1 aFavaron, Francesco1 aD'Ovidio, Renato  uhttps://library.ncl.res.in/content/pyramiding-pvpgip2-and-taxi-iii-not-pvpgip2-and-pmei-enhances-resistance-against-fusarium