01030nas a2200181   4500008004100000245009000041210006900131260000800200300001400208490000700222520038400229100002300613700001500636700003000651700001900681700002400700856012400724       2017                        eng d00aDendrimeric amide- and carbamate-linked lysine-based efficient molecular transporters0 aDendrimeric amide and carbamatelinked lysinebased efficient mole  cDEC  a9579-95840 v153 a<p>Amide- and carbamate-linked dendrimeric oligomers are reported as molecular transporters. They effectively complex with pDNA and transport it into cells at an efficiency superior to Lipofectamine, when complexation is carried out by incubation overnight. The carbamate-linked K2C is superior to amide-linked K2A; their pDNA complexes have very low associated cytotoxicity.</p>1 aYadav, Amit, Kumar1 aDey, Namit1 aChattopadhyay, Sabyasachi1 aGanguli, Munia1 aFernandes, Moneesha  uhttps://library.ncl.res.in/content/dendrimeric-amide-and-carbamate-linked-lysine-based-efficient-molecular-transporters02952nas a2200241   4500008004100000022001400041245011100055210006900166260000900235300001600244490000600260520209500266100002902361700002002390700002802410700002402438700002302462700002402485700002002509700002902529700002502558856012702583       2024                        eng d  a2470-134300aDiscovery of free glycated amines and glycated urea in diabetic plasma: potential implications in diabetes0 aDiscovery of free glycated amines and glycated urea in diabetic   cMAY   a24907-249150 v93 a<p>
	The role of protein glycation in the pathogenesis of diabetes has been well established. Akin to proteins, free amino acids and other small-molecule amines are also susceptible to glycation in hyperglycemic conditions and may have a role in the pathogenesis of the disease. However, information about glycation of free amino acids and other small-molecule amines is relatively obscure. In the quest to discover small-molecule glycated amines in the plasma, we have synthesized glycated amino acids, glycated creatine, and glycated urea, and by using a high-resolution accurate mass spectrometer, a mass spectral library was developed comprising the precursor and predominant fragment masses of glycated amines. Using this information, we report the discovery of the glycation of free lysine, arginine, and leucine/isoleucine from the plasma of diabetic patients. This has great physiological significance as glycation of these amino acids may create their deficiency and affect vital physiological processes such as protein synthesis, cell signaling, and insulin secretion. Also, these glycated amino acids could serve as potential markers of diabetes and its complications. While other amines, such as creatinine and urea, accumulate in the plasma and act as biomarkers of diabetic nephropathy. For the first time, we report the detection of glycated urea in diabetic plasma, which is confirmed by matching the precursor and fragment masses with the in vitro synthesized glycated urea by using C-12(6) and C-13(6)-glucose. Further, we quantified glycated urea detected in two forms, monoglycated urea (MGU) and diglycated urea (DGU), by a targeted mass spectrometric approach in the plasma of healthy, diabetic, and diabetic nephropathy subjects. Both MGU and DGU showed a positive correlation with clinical parameters, such as blood glucose and HbA1c. Given that urea gets converted to glycated urea in hyperglycemic conditions, it is crucial to quantify MGU and DGU along with the urea for the diagnosis of diabetic nephropathy and study their physiological role in diabetes.</p>
1 aShaikh, Rashdajabeen, Q.1 aDas, Sancharini1 aChaurasiya, Arvindkumar1 aAshtamy, Murali, G.1 aSheikh, Amreen, B.1 aFernandes, Moneesha1 aTiwari, Shalbha1 aUnnikrishnan, Ambika, G.1 aKulkarni, Mahesh, J.  uhttps://library.ncl.res.in/content/discovery-free-glycated-amines-and-glycated-urea-diabetic-plasma-potential-implications