02829nas a2200241   4500008004100000022001400041245011200055210006900167260000800236300001300244490000700257520196500264100002502229700002402254700002702278700002502305700002602330700002602356700002402382700002902406700002402435856012802459       2018                        eng d  a1935-273500aMetabolite profiling for biomarkers in schistosoma haematobium infection and associated bladder pathologies0 aMetabolite profiling for biomarkers in schistosoma haematobium i  cAPR  ae00064520 v123 a<p>Background Metabolic fingerprinting analysis can offer insights into underlying reactions in a biological system; hence it is crucial to the understanding of disease pathogenesis and could provide useful tools for discovering biomarkers. We sought to examine the urine and plasma metabolome in individuals affected by urogenital schistosomiasis and its associated-bladder pathologies. Methodology Blood and midstream urine were obtained from volunteers who matched our inclusion criteria among residents from Eggua, southwestern Nigeria. Samples were screened by urinalysis, microscopy, PCR and ultrasonography, and categorised as advanced (urogenital schistosomiasis associated-bladder pathologies), infection-only (urogenital schistosomiasis alone) and controls (no infection and no pathology). Metabolites were extracted and data acquired with ultra high-performance liquid chromatography coupled with Thermo Q-Exactive orbitrap HRMS. Data was analysed with MetaboAnalyst, Workflow4Metabolomics, HMDB, LipidMaps and other bioinformatics tools, with univariate and multivariate statistics for metabolite selection. Principal findings There were low levels of host sex steroids, and high levels of several benzenoids, catechols and lipids (including ganglioside, phosphatidylcholine and phosphatidylethanolamine), in infection-only and advanced cases (FDR&lt; 0.05, VIP&gt; 2, delta&gt; 2.0). Metabolites involved in biochemical pathways related to chorismate production were abundant in controls, while those related to choline and sphingolipid metabolism were upregulated in advanced cases FDR&lt;0.05). Some of these human host and Schistosoma haematobium molecules, including catechol estrogens, were good markers to distinguish infection-only and advanced cases. Conclusions Altered glycerophospholipid and sphingolipid metabolism could be key factors promoting the development of bladder pathologies and tumours during urogenital schistosomiasis.</p>1 aAdebayo, Adewale, S.1 aMundhe, Swapnil, D.1 aAwobode, Henrietta, O.1 aOnile, Olugbenga, S.1 aAgunloye, Atinuke, M.1 aIsokpehi, Raphael, D.1 aShouche, Yogesh, S.1 aSanthakumari, Bayatigeri1 aAnumudu, Chiaka, I.  uhttps://library.ncl.res.in/content/metabolite-profiling-biomarkers-schistosoma-haematobium-infection-and-associated-bladder02644nas a2200289   4500008004100000022001400041245008600055210006900141260000800210300001400218490000700232520174800239653001901987653001302006653001702019653001702036100001802053700001802071700002802089700002102117700002202138700002102160700002302181700002102204700002402225856010502249       2018                        eng d  a1466-502600aMicrobacterium telephonicum sp nov., isolated from the screen of a cellular phone0 aMicrobacterium telephonicum sp nov isolated from the screen of a  cAPR  a1052-10580 v683 a<p>A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63(T), exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234(T) and Microbacterium kitamiense Kitami C2(T). The DNA-DNA relatedness values of the strain S2T63(T) to M. aurantiacum KACC 20510(T), M. kitamiense KACC 20514(T) and Microbacterium laevaniformans KACC 14463(T) were 65% (+/- 4), 29.5% (+/- 3) and 55.9% (+/- 4), respectively. The genomic DNA G+C content was 71.8 mol%. The major fatty acids were anteiso C-15:0, iso-C-16 : 0, C-16 : 0 and anteiso-C-17 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids. The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1 alpha. The predominant menaquinones were MK-12 and MK-13. Rhamnose, fucose and galactose were the whole-cell sugars detected. The strain also showed biofilm production, estimated by using crystal violet assay. Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacterium telephonicum is proposed, with S2T63(T) (=MCC 2967 T = KACC 18715 T = LMG 29293(T)) as the type strain.</p>10aactinobacteria10abio-film10aMALDI-TOF MS10amobile phone1 aRahi, Praveen1 aKurli, Rashmi1 aPansare, Aabeejjeet, N.1 aKhairnar, Mitesh1 aJagtap, Shubhangi1 aPatel, Nisha, B.1 aDastager, Syed, G.1 aLawson, Paul, A.1 aShouche, Yogesh, S.  uhttps://library.ncl.res.in/content/microbacterium-telephonicum-sp-nov-isolated-screen-cellular-phone