02536nas a2200241   4500008004100000022001400041245012700055210006900182260007400251300001200325490000800337520161800345653002301963653002701986653002102013653001502034653002402049100002102073700002002094700002402114700003302138856012302171       2011                        eng d  a0167-685700aInfluence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L.0 aInfluence of endophytic fungal elicitation on production of inop  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcAUG  a345-3520 v1063 a<p>The influence of dried cell powder and culture filtrates of endophytic fungi on production of inophyllum in cell suspension cultures of leaf- and stem-derived callus of Calophyllum inophyllum was investigated. Two fungi, Nigrospora sphaerica and Phoma spp., endophytic to C. inophyllum, were isolated from leaf tissues, and were identified by both 18S rRNA gene amplification and sequencing. Elicitation of suspension cultures of both callus types of C. inophyllum with dried cell powder and culture filtrates of both fungi consistently elicited production of inophyllum A, B, C, and P. In comparison to stem-derived callus, suspension cultures of leaf-derived callus enhanced production of most inophyllum. Of the four inophyllum studied, the highest production of inophyllum A, C, and P was achieved in elicited suspension cultures of leaf-derived callus. Suspension cultures of stem-derived callus enhanced production only of inophyllum B. When suspension cultures of leaf-derived callus were elicited with 40 mg dried cell powder of Phoma spp., a level of 751-fold (6.84 mg/100 g elicited biomass) of inophyllum A was produced, compared to control. Whereas, a level of 414-fold (6.22 mg/100 g elicited biomass) of inophyllum B was produced when suspension cultures of stem-derived callus were elicited with 20 mg dried cell powder of N. sphaerica. When compared to control, a 10% culture filtrate of N. sphaerica in suspension cultures of leaf-derived callus elicited inophyllum C and P production by 928-fold (7.43 mg/100 g elicited biomass) and 750-fold (1.5 mg/100 g elicited biomass), respectively.</p>10aBiotic elicitation10aCalophyllum inophyllum10aEndophytic fungi10aInophyllum10aSuspension cultures1 aPawar, Kiran, D.1 aYadav, Amit, V.1 aShouche, Yogesh, S.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/influence-endophytic-fungal-elicitation-production-inophyllum-suspension-cultures-001733nas a2200277   4500008004100000022001400041245011000055210006900165260007900234300001200313490000800325520075200333653002201085653001701107653001501124653001101139653002401150653001301174100002501187700002201212700002701234700002401261700002401285700002101309856012501330       2011                        eng d  a0273-228900aIsolation, identification and optimization of a new extracellular lipase producing strain of rhizopus sp.0 aIsolation identification and optimization of a new extracellular  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcAUG  a969-9780 v1643 a<p>A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S-ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h, which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production.</p>10aCorn steep liquor10aFermentation10aITS region10aLipase10aMedium optimization10aRhizopus1 aKantak, Jayshree, B.1 aBagade, Aditi, V.1 aMahajan, Siddharth, A.1 aPawar, Shrikant, P.1 aShouche, Yogesh, S.1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/isolation-identification-and-optimization-new-extracellular-lipase-producing-strain-0