01613nas a2200217   4500008004100000022001400041245007100055210006900126260005700195300001100252490000700263520084700270653003001117653002501147653002801172100001901200700001701219700002801236700002601264856010501290       2010                        eng d  a0046-899100aProtoplast formation and regeneration in lactobacillus delbrueckii0 aProtoplast formation and regeneration in lactobacillus delbrueck  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcMAR  a97-1000 v503 a<p>Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa and Sharpe (MRS) with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with sucrose as an osmotic stabilizer using a combination of lysozyme (1 mg/ml) and mutanolysin (10 mu g/ml). Maximum protoplast regeneration was obtained on MRS medium with sucrose (0.5 M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of lactobacilli as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of L. delbrueckii.</p>10aLactobacillus delbrueckii10aProtoplast formation10aProtoplast regeneration1 aSinghvi, Mamta1 aJoshi, Dipti1 aGaikaiwari, Shalaka, A.1 aGokhale, Digambar, V.  uhttps://library.ncl.res.in/content/protoplast-formation-and-regeneration-lactobacillus-delbrueckii-0