01030nas a2200157   4500008004100000245008800041210006900129260000800198300001400206490000600220520046600226100002400692700001900716700002100735856011600756       2016                        eng d00aDraft genome sequence of arthrobacter enclensis NCIM 5488T for secondary metabolism0 aDraft genome sequence of arthrobacter enclensis NCIM 5488T for s  cJUN  ae00497-160 v43 aHere, we report the draft genome sequence of Arthrobacter enclensis NCIM 5488T, an actinobacterium isolated from a marine sediment sample from Chorao Island, Goa, India. This draft genome sequence consists of 4,226,231 bp with a G+C content of 67.08%, 3,888 protein-coding genes, 50 tRNAs, and 10 rRNAs. Analysis of the genome using bioinformatics tools such as antiSMASH and NaPDoS showed the presence of many unique natural product biosynthetic gene clusters.1 aNeurgaonkar, P., S.1 aDharne, M., S.1 aDastager, S., G.  uhttps://library.ncl.res.in/content/draft-genome-sequence-arthrobacter-enclensis-ncim-5488t-secondary-metabolism02575nas a2200181   4500008004100000245013100041210006900172260000800241300001100249490000600260520190300266100001802169700002402187700001702211700002102228700001902249856012502268       2016                        eng d00aPeeping into genomic architecture by re-sequencing of Ochrobactrum intermedium M86 strain during laboratory adapted conditions0 aPeeping into genomic architecture by resequencing of Ochrobactru  cJUN  a 72-760 v83 aAdvances in de novo sequencing technologies allow us to track deeper insights into microbial genomes for restructuring events during the course of their evolution inside and outside the host. Bacterial species belonging to Ochrobactrum genus are being reported as emerging, and opportunistic pathogens in this technology driven era probably due to insertion and deletion of genes. The Ochrobactrum intermedium M86 was isolated in 2005 from a case of non-ulcer dyspeptic human stomach followed by its first draft genome sequence in 2009. Here we report re-sequencing of O. intermedium M86 laboratory adapted strain in terms of gain and loss of genes. We also attempted for finer scale genome sequence with 10 times more genome coverage than earlier one followed by comparative evaluation on Ion PGM and Illumina MiSeq. Despite their similarities at genomic level, lab-adapted strain mainly lacked genes encoding for transposase protein, insertion elements family, phage tail-proteins that were not detected in original strain on both chromosomes. Interestingly, a 5 kb indel was detected in chromosome 2 that was absent in original strain mapped with phage integrase gene of Rhizobium spp. and may be acquired and integrated through horizontal gene transfer indicating the gene loss and gene gain phenomenon in this genus. Majority of indel fragments did not match with known genes indicating more bioinformatic dissection of this fragment. Additionally we report genes related to antibiotic resistance, heavy metal tolerance in earlier and re-sequenced strain. Though SNPs detected, there did not span urease and flagellar genes. We also conclude that third generation sequencing technologies might be useful for understanding genomic architecture and re-arrangement of genes in the genome due to their ability of larger coverage that can be used to trace evolutionary aspects in microbial system. 1 aGohil, K., N.1 aNeurgaonkar, P., S.1 aParanjpe, A.1 aDastager, S., G.1 aDharne, M., S.  uhttps://library.ncl.res.in/content/peeping-genomic-architecture-re-sequencing-ochrobactrum-intermedium-m86-strain-during01941nas a2200181   4500008004100000245014400041210006900185260000800254300002300262490000700285520124700292100002001539700001601559700001701575700001801592700001901610856013001629       2019                        eng d00aCharacterization of novel acidic and thermostable phytase secreting Streptomyces sp. (NCIM 5533) for plant growth promoting characteristics0 aCharacterization of novel acidic and thermostable phytase secret  cMAR  aArticle No: 1010200 v183 aSuccessful agricultural practices in acidic and arid region soils are still challenging worldwide. We investigated Actinomycete strain obtained from soil, which was found to produce extracellular phytase having the optimum pH, temperature of 2.5 and 70 °C, respectively and Plant growth promoting (PGP) attributes. These abilities of the phytase make it novel as compared to other Streptomyces derived phytases. Strain was also evaluated for physiological traits such as salinity, temperature, and pH followed by morphological and molecular identification. The Streptomyces sp. strain (NCIM 5533) displayed additional PGP traits such as indole-3-acetic acid (IAA), ammonia and phosphate solubilization activity. A significant increment in Tomato plant (Solanum lycopersicum) root, shoot and as well as total height in bioassays at laboratory scale and green house level and its ability to colonize roots substantiated its potency as a true plant growth promoting rhizobacteria (PGPR). To our knowledge this is the first report of Streptomyces sp. having the ability to produce acidic and thermostable phytase as well as PGP traits showing enhanced plant growth could have implications in soils having acidic nature and also in arid region.1 aPuppala, K., R.1 aBhavsar, K.1 aSonalkar, V.1 aKhire, J., M.1 aDharne, M., S.  uhttps://library.ncl.res.in/content/characterization-novel-acidic-and-thermostable-phytase-secreting-streptomyces-sp-ncim-5533