02558nas a2200253   4500008004100000022001400041245009000055210006900145260000800214300001200222490000700234520173800241653001901979653001301998653001802011653002302029653001602052653002602068100001602094700002502110700002702135700002402162856011802186       2025                        eng d  a0971-781100aHarnessing microRNAs and their targets for salt stress tolerance in Pandanus odorifer0 aHarnessing microRNAs and their targets for salt stress tolerance  cSEP  a714-7300 v343 a<p>
	Salinity poses a formidable challenge to agriculture, detrimentally impacting plant growth and the yield of several crops globally. Pandanus odorifer (Forssk.) Kuntze is a highly salt-stress resilient plant that grows naturally along the coastal regions of Asia, Southeast Asia, and Polynesia. It is frequently found as a frontier species along the sandy shores receiving constant salt sprays. MicroRNAs (miRNAs) play essential regulatory roles in growth, development, and combating various biotic and abiotic stresses. Thus, characterizing miRNAs and their targets is critical to understanding their roles in salt stress tolerance. Through a rigorous analysis based on homology, secondary structure and signatures of other miRNA genes, we identified 52 conserved nonredundant miRNAs from the P. odorifer transcriptome data. These miRNAs were diverse and belonged to 23 miRNA phylogenetic families. Several miRNAs, including pod-MIR156a, pod-MIR164d, and pod-MIR535d, demonstrated distinct expression patterns in response to salt stress. Their target genes exhibited opposing expression trends, corroborating the intricate miRNA-target relationship. Delving deeper into their influence, we discovered that these miRNAs mainly targeted transcription factors that are known for their pivotal roles in stress responses. We also detected four miRNA-star (miRNA*) sequences (the complementary strands of functional mature miRNAs), which supports the authenticity of the identified miRNAs. This study unveils the intricate regulatory mechanism through which miRNAs may reinforce salt stress tolerance in P. odorifer, providing a promising avenue for enhancing crop resilience to various stresses through miRNA-based strategies.</p>
10aAbiotic stress10amicroRNA10amiRNA targets10aSalinity tolerance10asalt stress10aTranscription factors1 aRashmi, Deo1 aBarvkar, Vitthal, T.1 aNadaf, Altafhusain, B.1 aKadoo, Narendra, Y.  uhttps://library.ncl.res.in/content/harnessing-micrornas-and-their-targets-salt-stress-tolerance-pandanus-odorifer