01465nas a2200217   4500008004100000022001400041245012800055210006900183260000800252300001400260490000800274520068300282100002500965700002400990700002001014700002201034700002401056700002401080700002101104856012201125       2018                        eng d  a1523-706000aTotal synthesis and biological evaluation of cell adhesion inhibitors peribysin A and B: structural revision of peribysin B0 aTotal synthesis and biological evaluation of cell adhesion inhib  cNOV  a7003-70060 v 203 aTotal synthesis of potent cell-adhesion inhibitors peribysins A and B has been accomplished for the first time in racemic form. A Diels-Alder/aldol sequence to build the skeleton and decoration of the desired functionalities of the targeted natural products using highly stereoselective operations are the highlights. The structures of synthesized peribysins were fully characterized using spectral data and single-crystal X-ray analysis. Through this total synthesis, the initially proposed structure of peribysin B has been revised. Furthermore, the cell-adhesion inhibition potential of the scaffold (two peribysins + three analogues) was confirmed using anti-adhesion assay.1 aKalmode, Hanuman, P.1 aHandore, Kishor, L.1 aRajput, Raveena1 aShaikh, Samir, R.1 aGonnade, Rajesh, G.1 aKulkarni, Kiran, A.1 aReddy, Srinivasa  uhttps://library.ncl.res.in/content/total-synthesis-and-biological-evaluation-cell-adhesion-inhibitors-peribysin-and-b01311nas a2200253   4500008004100000022001400041245011000055210006900165260000800234300001100242490000700253520044700260653001700707653002400724653002200748653002300770100002600793700002000819700002000839700002100859700002400880700002400904856012900928       2022                        eng d  a0960-894X00aPostmodification of voxelotor (GBT 440) via [Rh]-catalyzed cross dehydrogenative coupling with olefins br0 aPostmodification of voxelotor GBT 440 via Rhcatalyzed cross dehy  cDEC  a1290220 v773 a<p>
	The directed Rh(III)-catalyzed cross dehydrogenative coupling of the pyrazole unit of the GBT-440 scaffold has been explored with simple as well as conjugated olefins to synthesize post-functionalized GBT-440 analogues. The screening of these synthesized compounds for improving the oxygen binding efficiency of the hemoglobin isolated from the sickled red blood cells revealed that some of these compounds are as good as GBT-440.</p>
10aCDC Coupling10aGBT-440 (Voxelotor)10aRh(III) catalysis10asickle cell anemia1 aKalshetti, Rupali, G.1 aRajput, Raveena1 aMotiwala, Zenia1 aReddy, Srinivasa1 aKulkarni, Kiran, A.1 aRamana, Chepuri, V.  uhttps://library.ncl.res.in/content/postmodification-voxelotor-gbt-440-rh-catalyzed-cross-dehydrogenative-coupling-olefins-br03041nas a2200193   4500008004100000245013000041210006900171260000900240300001400249490000700263520232300270100001902593700002202612700001902634700002002653700002002673700002402693856013002717       2023                        eng d00aCocrystallization of multi-kinase inhibitor pazopanib with fenamic acids: improving dissolution and inhibiting cell migration0 aCocrystallization of multikinase inhibitor pazopanib with fenami  cOCT   a5565-55740 v253 a<p>
	A multi-kinase inhibitor, pazopanib (PAZ) is cocrystallized with cyclooxygenase (COX) inhibitor fenamic acids to investigate the dissolution rate and inhibition of cell migration in VEGF-triggered HUVEC cells to test the efficacy of stoichiometric drug-drug combinations. Crystallization experiments at the sub-milligram level in an acetonitrile-methanol mixture yielded two drug-drug salt forms of PAZ with flufenamic acid (FFA) and niflumic acid (NFA) as PAZ+center dot FFA-center dot ACN (an acetonitrile solvate named as form I) and PAZ+center dot NFA-. Structures of the crystal forms were characterized by single crystal X-ray diffraction (SC-XRD) method. Crystal structures revealed that the presence of a 2-aminopyrimidine group in PAZ is a strong partner for the carboxyl group in all solid forms, forming an acidMIDLINE HORIZONTAL ELLIPSISpyrimidine heterosynthon with COX inhibitor fenamic acids. To perform dissolution experiments and cell line analysis, the scale-up of both salt forms were done in the acetonitrile-methanol mixture through crystallization, which showed a polymorphic transformation in the case of PAZ+center dot FFA-center dot ACN (an acetonitrile solvate named form II). The thermodynamic stability of PAZ+center dot FFA-center dot ACN (form II) and PAZ+center dot NFA- were analysed using slurry experiment under ambient conditions in pH 1.2 (0.1 N HCl) buffer medium and the residual solid phase was characterized by powder XRD, which showed that PAZ+center dot FFA-center dot ACN (form II) was a metastable solid form while PAZ+center dot NFA- was a stable solid form. The dissolution experiments at gastric pH 1.2 showed that the rate of dissolution of PAZ+center dot FFA-center dot ACN was 10 times higher than PAZ+center dot NFA-. The cell migration assay suggested that PAZ+center dot FFA-center dot ACN inhibited similar to 25% and PAZ+center dot NFA- inhibited similar to 20% migration of HUVEC cells compared to PAZ alone. These investigations suggested that the drug-drug salts PAZ+center dot FFA- and PAZ+center dot NFA- would be potential combo drug candidates for clinical trials. Multi-drug crystalline salts of pazopanib with fenamic acids exhibit enhanced dissolution and 20% higher inhibition in the migration of HUVEC cells compared to the reference drug.</p>
1 aRai, Sunil, K.1 aGunnam, Anilkumar1 aRoy, Debopriya1 aRajput, Raveena1 aKulkarni, Kiran1 aNangia, Ashwini, K.  uhttps://library.ncl.res.in/content/cocrystallization-multi-kinase-inhibitor-pazopanib-fenamic-acids-improving-dissolution-and