01802nas a2200193   4500008004100000022001400041245010800055210006900163260000800232490000700240520109100247100002401338700002401362700002501386700002401411700002101435700002801456856012401484       2018                        eng d  a2045-232200aEvaluating antimalarial efficacy by tracking glycolysis in Plasmodium falciparum using NMR spectroscopy0 aEvaluating antimalarial efficacy by tracking glycolysis in Plasm  cDEC0 v 83 aGlucose is an essential nutrient for Plasmodium falciparum and robust glycolytic activity is indicative of viable parasites. Using NMR spectroscopy, we show that P. falciparum infected erythrocytes consume similar to 20 times more glucose, and trophozoites metabolize similar to 6 times more glucose than ring stage parasites. The glycolytic activity, and hence parasite viability, can be measured within a period of 2 h to 5 h, using this method. This facilitates antimalarial bioactivity screening on ring and trophozoite stage parasites, exclusively. We demonstrate this using potent and mechanistically distinct antimalarial compounds such as chloroquine, atovaquone, cladosporin, DDD107498 and artemisinin. Our findings indicate that ring stage parasites are inherently more tolerant to antimalarial inhibitors, a feature which may facilitate emergence of drug resistance. Thus, there is a need to discover novel antimalarial compounds, which are potent and fast acting against ring stage parasites. The NMR method reported here can facilitate the identification of such molecules.1 aShivapurkar, Rupali1 aHingamire, Tejashri1 aKulkarni, Akshay, S.1 aRajamohanan, P., R.1 aReddy, Srinivasa1 aShanmugam, Dhanasekaran  uhttps://library.ncl.res.in/content/evaluating-antimalarial-efficacy-tracking-glycolysis-plasmodium-falciparum-using-nmr03085nas a2200577   4500008004100000022001400041245011300055210006900168260000800237300000700245490000700252520136700259653001001626653001601636653001701652653000801669653001101677653001001688653002001698100002301718700002601741700003301767700002501800700002001825700002001845700002001865700002201885700002201907700002101929700002201950700002201972700002201994700001802016700002402034700002402058700001602082700002802098700001902126700001402145700002802159700002002187700002402207700002202231700002502253700002002278700002002298700002102318700001502339700002302354856013002377       2025                        eng d  a1573-388200aRecommendations for sample selection, collection and preparation for NMR-based metabolomics studies of blood0 aRecommendations for sample selection collection and preparation   cMAY  a660 v213 a<p>
	BackgroundMetabolic profiling of blood metabolites, particularly in plasma and serum, is vital for studying human diseases, human conditions, drug interventions and toxicology. The clinical significance of blood arises from its close ties to all human cells and facile accessibility. However, patient-specific variables such as age, sex, diet, lifestyle and health status, along with pre-analytical conditions (sample handling, storage, etc.), can significantly affect metabolomic measurements in whole blood, plasma, or serum studies. These factors, referred to as confounders, must be mitigated to reveal genuine metabolic changes due to illness or intervention onset.Review objectiveThis review aims to aid metabolomics researchers in collecting reliable, standardized datasets for NMR-based blood (whole/serum/plasma) metabolomics. The goal is to reduce the impact of confounding factors and enhance inter-laboratory comparability, enabling more meaningful outcomes in metabolomics studies.Key conceptsThis review outlines the main factors affecting blood metabolite levels and offers practical suggestions for what to measure and expect, how to mitigate confounding factors, how to properly prepare, handle and store blood, plasma and serum biosamples and how to report data in targeted NMR-based metabolomics studies of blood, plasma and serum.</p>
10aBlood10aMetabolites10aMetabolomics10aNMR10aPlasma10aSerum10aStandardization1 aEmwas, Abdul-Hamid1 aZacharias, Helena, U.1 aAlborghetti, Marcos, Rodrigo1 aGowda, G., A. Nagana1 aRaftery, Daniel1 aMckay, Ryan, T.1 aChang, Chung-ke1 aSaccenti, Edoardo1 aGronwald, Wolfram1 aSchuchardt, Sven1 aLeiminger, Roland1 aMerzaban, Jasmeen1 aMadhoun, Nour, Y.1 aIqbal, Mazhar1 aAlsiary, Rawiah, A.1 aShivapurkar, Rupali1 aPain, Arnab1 aShanmugam, Dhanasekaran1 aRyan, Danielle1 aRoy, Raja1 aSchirra, Horst, Joachim1 aMorris, Vanessa1 aZeri, Ana, Carolina1 aAlahmari, Fatimah1 aKaddurah-Daouk, Rima1 aSalek, Reza, M.1 aLeVatte, Marcia1 aBerjanskii, Mark1 aLee, Brian1 aWishart, David, S.  uhttps://library.ncl.res.in/content/recommendations-sample-selection-collection-and-preparation-nmr-based-metabolomics-studies