01721nas a2200265 4500008004100000022001400041245007400055210006900129260000800198300001200206490000700218520090400225653001901129653001401148653001501162653002701177653001001204100002201214700002401236700002101260700002601281700002601307700002301333856009901356 2018 eng d a0935-964800aIn situ nanoparticle embedding for authentication of epoxy composites0 aIn situ nanoparticle embedding for authentication of epoxy compo cAUG a18015230 v303 a

In situ reduction of chloroauric acid inside an amine-cured epoxy matrix leads to formation of gold nanoparticles which are embedded inside the part. This phenomenon is leveraged to design an authentication system for composites wherein the particles are embedded spatially and are invisible to the naked eye. Under UV light, the particles diffract light and create an easily visible path. The particles penetrate inside the part and create a permanent, cost-effective, tamper-proof code. The advantage of this technique is that this authentication system can be built in composite parts after fabrication of the composite structure. As very small amount (nanograms) of particles are present in the part, negligible change in the thermal characteristics of the parent matrix is observed. The particles can be embedded easily in carbon fiber as well as glass fiber reinforced epoxy structures.

10aauthentication10abarcoding10acomposites10aembedded nanoparticles10aEpoxy1 aPansare, Amol, V.1 aKhairkar, Shyam, R.1 aShedge, Amol, A.1 aChhatre, Shraddha, Y.1 aPatil, Vishwanath, R.1 aNagarkar, Amit, A. uhttps://library.ncl.res.in/content/situ-nanoparticle-embedding-authentication-epoxy-composites02026nas a2200277 4500008004100000022001400041245012700055210006900182260000800251300001100259490000800270520106100278653003201339653002401371653001901395653003301414100002401447700000501471700002101476700002601497700001901523700002801542700002601570700002301596856012901619 2020 eng d a0045-653500aHydrophobic interpenetrating polyamide-PDMS membranes for desalination, pesticides removal and enhanced chlorine tolerance0 aHydrophobic interpenetrating polyamidePDMS membranes for desalin cNOV a1271790 v2583 a

Hydrophobic membranes for desalination and toxic organic pollutant removal have been fabricated using polyamide - PDMS (polydimethylsiloxane) chemistries in a one-step protocol. The curing of polyamide and PDMS are orthogonal and co-curing both networks imparts hydrophobicity to the thin film composite membranes. The membranes exhibit increased adsorption of pesticides from the feed water along with maintaining excellent salt rejection capability (97% NaCl rejection), thus giving the membranes a multifunctional character. Three toxic pesticides have been used in this study to demonstrate the viability of combining osmosis desalination technology with organic matter adsorption. The membranes also show excellent resistance to fouling by toxic pesticides (85% salt rejection vs 67% for commercial membranes in the presence of pesticides) and significantly improved chlorine tolerance (93.8% salt rejection vs 86.5% for commercial membranes after 20 h of exposure to sodium hypochlorite solution). (C) 2020 Elsevier Ltd. All rights reserved.

10aPDMS (Polydimethylsiloxane)10aPesticide rejection10aSalt rejection10aWater purification membranes1 aKhairkar, Shyam, R.1 a1 aShedge, Amol, A.1 aChhatre, Shraddha, Y.1 aSuresh, A., K.1 aChakrabarti, Subhananda1 aPatil, Vishwanath, R.1 aNagarkar, Amit, A. uhttps://library.ncl.res.in/content/hydrophobic-interpenetrating-polyamide-pdms-membranes-desalination-pesticides-removal-and02808nas a2200289 4500008004100000022001400041245012000055210006900175260000800244300001100252490000800263520181600271653003102087653001802118653002202136653002402158653001802182100002102200700002502221700002402246700002602270700002002296700002302316700002202339700002602361856013102387 2020 eng d a0162-013400aNanocomposite of functional silver metal containing curcumin biomolecule model systems: Protein BSA bioavailability0 aNanocomposite of functional silver metal containing curcumin bio cNOV a1112100 v2123 a

Curcumin, a constituent of Curcuma longa L-Zingiberaceae is used in traditional Indian and worldwide medicine and shows anticancer and antioxidant properties. Curcumin has numerous biological and pharmacological ac-tivities but due to its hydrophobic nature, the major drawback is poor absorption and rapid elimination, rendering curcumin with the tag of a poor biomaterial. Hence, there is a need to develop functional metal containing curcumin model systems (FMCCMS) as a metallo-biomolecule to enhance the bioavailability of curcumin. We designed the interaction of silver metal ion with curcumin to form curcumin-silver nanocomposite (CURC-AgNCP) via ultrasonic synthetic route. Formations of FMCCMS were characterized by spectroscopic techniques. The crystalline face-centered cubic pattern and particle size of the nanocomposite was evaluated using X-ray diffraction and high-resolution transmission electron microscopy. The bonding of silver metal to curcumin was confirmed by X-ray photon spectroscopy. Interaction of the nanocomposite with bovine serum albumin (BSA) protein was performed using excitation, emission, and circular dichroism spectroscopy. In binding interaction of BSA, the negative value of Delta S degrees (-358.04 J mol(-1) K-1) and Delta H degrees (-129.42 KJ mol(-1)) demonstrates the hydrophilic nature of the nanocomposite. The binding distance r evaluated according to the Forster resonance energy transfer theory and was 4.69 nm for CURC-AgNCP, which suggested non-radiative transfer of energy between CURC-AgNCP and BSA. The role of FMCCMS metallo-biomolecule CURC-AgNCP in medicine for cancer activity can have immense importance and hence we performed Sulphorhodamine B based in-vitro cytotoxicity assay on human breast cancer Michigan Cancer Foundation-7 cell line.

10aAg-based CURC biomaterials10aBreast cancer10aMetal in medicine10aMetallobiomolecules10aNanocomposite1 aShedge, Amol, A.1 aPansare, Shubham, V.1 aKhairkar, Shyam, R.1 aChhatre, Shraddha, Y.1 aChakrabarti, S.1 aNagarkar, Amit, A.1 aPansare, Amol, V.1 aPatil, Vishwanath, R. uhttps://library.ncl.res.in/content/nanocomposite-functional-silver-metal-containing-curcumin-biomolecule-model-systems-protein02127nas a2200265 4500008004100000022001400041245010800055210006900163260000800232300001400240490000700254520123000261653001501491653001701506653001601523653003801539100002401577700002101601700002101622700002201643700002701665700002601692700001801718856012501736 2022 eng d a0170-083900aBiological macromolecule chitosan grafted co-polymeric composite: bio-adsorption probe on cationic dyes0 aBiological macromolecule chitosan grafted copolymeric composite  cNOV a9441-94550 v793 a

Chitosan biological macromolecule is a versatile polymer; chemical modification has been carried out that lead to the formation of chitosan grafted polymers composites (Chito-g-PC). We proposed synthesis of six various Chito-g-PC as sorbents for toxic dyes. A novel graft copolymerization method based on radical polymerization with vinyl monomer like acrylic acid, acrylamide, N-isopropylacrylamide, methacrylic acid and polyacrylonitrile were utilized in order to address the large amount of swelling at four different pH buffers solution. The effect of initiator and monomer concentration, time and temperature on % grafting and % grafting efficiency were performed. Comparative characterization of Chito and Chito-g-PC were evaluated by SEM, XRD and FTIR, as well as solubility characteristics of the composites were determined by various pH buffer solution. Cationic toxic dyes Malachite green (MG) and Methylene blue (MB) were selected as the sorbet, and Chito-g-PC were used as biosorbents. Thermodynamic analysis showed that the sorption process was spontaneous and endothermic with an increased randomness. The sorption experiments were realized with six different Chito-g-PC for MG and MB at various pH.

10aAcrylamide10aacrylic acid10aBio sorbent10aChitosan biological macromolecule1 aKhairkar, Shyam, R.1 aPansare, Shubham1 aShedge, Amol, A.1 aChhatre, Shraddha1 aKulal, Dnyaneshwar, K.1 aPatil, Vishwanath, R.1 aPansare, Amol uhttps://library.ncl.res.in/content/biological-macromolecule-chitosan-grafted-co-polymeric-composite-bio-adsorption-probe02107nas a2200229 4500008004100000245009700041210006900138260000800207300001400215490000700229520131000236100001801546700002101564700002701585700002101612700002401633700002401657700002601681700002701707700002601734856011701760 2022 eng d00aDeciphering the sensing of alpha-amyrin acetate with hs-DNA: a multipronged biological probe0 aDeciphering the sensing of alphaamyrin acetate with hsDNA a mult cJAN a1238-12430 v123 aIn this study, we focus on the biomimetic development of small molecules and their biological sensing with DNA. The binding of herring sperm deoxyribonucleic acid (hs-DNA) with naturally occurring bioactive small molecule alpha-amyrin acetate (alpha-AA), a biomimetic - isolated from the leaves of Ficus (F.) arnottiana is investigated. Collective information from various imaging, spectroscopic and biophysical experiments provides evidence that alpha-AA is a minor groove sensor of hs-DNA and preferentially binds to the A-T-rich regions. Interactions of different concentrations of small molecule alpha-AA with hsDNA were evaluated via various analytical techniques such as UV-Vis, circular dichroism (CD) and fluorescence emission spectroscopy. Fluorescence emission spectroscopy results suggest that alpha-AA decreases the emission level of hsDNA. DNA minor groove sensor Hoechst 33258 and intercalative sensor EB, melting transition analysis (T-M) and viscosity analysis clarified that alpha-AA binds to hs-DNA via a groove site. Biophysical chemistry and molecular docking studies show that hydrophobic interactions play a major role in this binding. The present research deals with a natural product biosynthesis-linked chemical-biology interface sensor as a biological probe for alpha-AA: hs-DNA.1 aPansare, Amol1 aShedge, Amol, A.1 aSonawale, Maryappa, C.1 aPansare, Shubham1 aMahakal, Akshay, D.1 aKhairkar, Shyam, R.1 aChhatre, Shraddha, Y.1 aKulal, Dnyaneshwar, K.1 aPatil, Vishwanath, R. uhttps://library.ncl.res.in/content/deciphering-sensing-alpha-amyrin-acetate-hs-dna-multipronged-biological-probe01897nas a2200205 4500008004100000245011300041210006900154260000800223300001100231490000600242520113500248100002701383700002501410700002701435700002501462700002801487700002601515700002201541856012801563 2025 eng d00aDevelopment of reversed-phase HPLC method for purity assessment of aryl porphyrins and their metal complexes0 aDevelopment of reversedphase HPLC method for purity assessment o cMAY ae700580 v83 a

A gradient reversed-phase HPLC method was developed for in-process monitoring of the formation of aryl porphyrins and their corresponding metallo-aryl porphyrins. The method also proved effective for determining their quantitative purity. Optimal results achieving proper peak shape and separation of peaks in the chromatogram were obtained using C8 column and gradient of acetic acid-water and acetonitrile. The detector wavelength was selected for simultaneous detection of starting materials and products for in-process analysis for conversion of aryl aldehydes to corresponding tetraaryl porphyrins at 254 nm and metallo-aryl porphyrins formation from tetraaryl porphyrin at 413 nm, respectively; while the purity of products could be determined at 413 nm. The developed method is efficient, versatile, accurate, and easy to carry out and could serve as a handy tool for the reaction monitoring and determination of purity in the processes development of synthetic porphyrins and metalloporphyrins.

1 aUjagare, Ashish, Mohan1 aUzagare, Matthew, C.1 aGhogare, Bhausaheb, N.1 aGupta, Lokesh, Kumar1 aWadgaonkar, Prakash, P.1 aPatil, Vishwanath, R.1 aDonde, Kamini, J. uhttps://library.ncl.res.in/content/development-reversed-phase-hplc-method-purity-assessment-aryl-porphyrins-and-their-metal02374nas a2200325 4500008004100000022001400041245006100055210005400116260000800170300001200178490000700190520139900197653001901596653001401615653002701629653002101656653003101677653002201708100002201730700002601752700002501778700002001803700002101823700002201844700002201866700002201888700002601910700002301936856008901959 2025 eng d a2365-709X00aX-ray fluorescence-based spray-on ``elemental barcodes''0 aXray fluorescencebased sprayon elemental barcodes cJUL a24016870 v103 a

A novel spray-on elemental barcode technology is reported for the secure, permanent, and tamper-proof identification of polymeric and fiber-reinforced composite materials. This system utilizes the in situ reduction of metal salts to nanoparticles embedded within a polymer matrix, creating a unique barcode that is detectable through X-ray fluorescence (XRF). The barcode's composition is based on the metallic nanoparticle mixture and offers a semi-quantitative, non-destructive, and thermally stable method for material authentication. The process is straightforward, involving the manual application of metal salt solutions followed by mild heating, ensuring no significant alteration to the material's properties. The barcodes can be read through protective coatings or paints and are robust even under extreme conditions, such as high temperatures. This low-cost method does not introduce additional manufacturing steps and demonstrates significant potential for anti-counterfeiting and lifecycle tracking in various industries. Moreover, with an information density of up to 12 bits mm-1, this elemental barcode significantly surpasses the data storage capabilities of traditional optical barcodes. This approach holds promise for broad substrate applicability and can be expanded to other metals and reduction protocols, making it versatile for diverse material applications.

10aauthentication10abarcoding10aembedded nanoparticles10aepoxy thermosets10afiber-reinforced composite10ain situ reduction1 aPansare, Amol, V.1 aTerrasi, Giovanni, P.1 aPansare, Shubham, V.1 aKhairkar, Shyam1 aShedge, Amol, A.1 aZote, Santosh, W.1 aChhatre, Shraddha1 aBarbezat, Michael1 aPatil, Vishwanath, R.1 aNagarkar, Amit, A. uhttps://library.ncl.res.in/content/x-ray-fluorescence-based-spray-elemental-barcodes