02808nas a2200289   4500008004100000022001400041245012000055210006900175260000800244300001100252490000800263520181600271653003102087653001802118653002202136653002402158653001802182100002102200700002502221700002402246700002602270700002002296700002302316700002202339700002602361856013102387       2020                        eng d  a0162-013400aNanocomposite of functional silver metal containing curcumin biomolecule model systems: Protein BSA bioavailability0 aNanocomposite of functional silver metal containing curcumin bio  cNOV  a1112100 v2123 a<p>Curcumin, a constituent of Curcuma longa L-Zingiberaceae is used in traditional Indian and worldwide medicine and shows anticancer and antioxidant properties. Curcumin has numerous biological and pharmacological ac-tivities but due to its hydrophobic nature, the major drawback is poor absorption and rapid elimination, rendering curcumin with the tag of a poor biomaterial. Hence, there is a need to develop functional metal containing curcumin model systems (FMCCMS) as a metallo-biomolecule to enhance the bioavailability of curcumin. We designed the interaction of silver metal ion with curcumin to form curcumin-silver nanocomposite (CURC-AgNCP) via ultrasonic synthetic route. Formations of FMCCMS were characterized by spectroscopic techniques. The crystalline face-centered cubic pattern and particle size of the nanocomposite was evaluated using X-ray diffraction and high-resolution transmission electron microscopy. The bonding of silver metal to curcumin was confirmed by X-ray photon spectroscopy. Interaction of the nanocomposite with bovine serum albumin (BSA) protein was performed using excitation, emission, and circular dichroism spectroscopy. In binding interaction of BSA, the negative value of Delta S degrees (-358.04 J mol(-1) K-1) and Delta H degrees (-129.42 KJ mol(-1)) demonstrates the hydrophilic nature of the nanocomposite. The binding distance r evaluated according to the Forster resonance energy transfer theory and was 4.69 nm for CURC-AgNCP, which suggested non-radiative transfer of energy between CURC-AgNCP and BSA. The role of FMCCMS metallo-biomolecule CURC-AgNCP in medicine for cancer activity can have immense importance and hence we performed Sulphorhodamine B based in-vitro cytotoxicity assay on human breast cancer Michigan Cancer Foundation-7 cell line.</p>
10aAg-based CURC biomaterials10aBreast cancer10aMetal in medicine10aMetallobiomolecules10aNanocomposite1 aShedge, Amol, A.1 aPansare, Shubham, V.1 aKhairkar, Shyam, R.1 aChhatre, Shraddha, Y.1 aChakrabarti, S.1 aNagarkar, Amit, A.1 aPansare, Amol, V.1 aPatil, Vishwanath, R.  uhttps://library.ncl.res.in/content/nanocomposite-functional-silver-metal-containing-curcumin-biomolecule-model-systems-protein