02373nas a2200265   4500008004100000022001400041245011300055210006900168260007300237300001000310490000800320520140200328653001601730653002401746653002101770653002501791653002301816100002101839700002401860700002501884700002501909700002401934700002001958856012901978       2015                        eng d  a0927-776500aBio-physical evaluation and in vivo delivery of plant proteinase inhibitor immobilized on silica nanospheres0 aBiophysical evaluation and in vivo delivery of plant proteinase   aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUN  a84-920 v1303 a<p>Recombinant expression of Capsicum annuum proteinase inhibitors (CanPI-13) and its application via synthetic carrier for the crop protection is the prime objective of our study. Herein, we explored proteinase inhibitor peptide immobilization on silica based nanospheres and rods followed by its pH mediated release in vitro and in vivo. Initial studies suggested silica nanospheres to be a suitable candidate for peptide immobilization. Furthermore, the interactions were characterized biophysically to ascertain their conformational stability and biological activity. Interestingly, bioactive peptide loading at acidic pH on nanospheres was found to be 62% and showed 56% of peptide release at pH 10, simulating gut milieu of the target pest Helicoverpa armigera. Additionally, in vivo study demonstrated significant reduction in insect body mass (158 mg) as compared to the control insects (265 mg) on 8th day after feeding with CanPI-13 based silica nanospheres. The study confirms that peptide immobilized silica nanosphere is capable of affecting overall growth and development of the feeding insects, which is known to hamper fecundity and fertility of the insects. Our study illustrates the utility and development of peptide-nanocarrier based platform in delivering diverse biologically active complexes specific to gut pH of H. armigera. (C) 2015 Elsevier B.V. All rights reserved.</p>10aBiodelivery10aHelicovera armigera10aProtein `corona'10aProteinase inhibitor10aSilica nanospheres1 aKhandelwal, Neha1 aDoke, Dhananjay, S.1 aKhandare, Jayant, J.1 aJawale, Priyanka, V.1 aBiradar, Ankush, V.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/bio-physical-evaluation-and-vivo-delivery-plant-proteinase-inhibitor-immobilized-silica-0