02050nas a2200181 4500008004100000022001500041245012300056210006900179260000800248300001200256490000800268520138600276100002101662700001701683700001801700700001801718856013201736 2018 eng d a 0926-669000a In vitro propagation of Digitalis lanata Ehrh. through direct shoot regeneration - A source of cardiotonic glycosides0 aIn vitro propagation of Digitalis lanata Ehrh through direct sho cOCT a313-3190 v1213 a

An efficient method for the in vitro propagation of Digitalis lanata Ehrh. through direct organogenesis from leaf and petiole explants has been standardised. MS basal medium supplemented with cytokinins BAP, KIN, TDZ either alone or along with auxins IAA and NAA at different concentrations were tried. TDZ at 4.54 and 6.81 μmol/l were optimum for direct regeneration of shoots from leaf (4.4 ± 0.6 shoots/explant), and petiole (3.0 ± 0.8 shoots/explant) explants respectively. Among the various concentrations of auxins IAA, IBA and NAA tried for rooting, the best response occurred on MS basal medium supplemented with 17.13 μmol/l IAA. On greenhouse transfer about 60% of the plantlets survived. In vitro raised plantlets were morphologically similar to mother plants. Cardiotonic glycosides digoxin and digitoxin were extracted by modified methods and estimated by HPLC. There were no significant differences in digoxin and digitoxin content in leaves of naturally grown and in vitro raised plants. The method for in vitro propagation of D. lanata through direct organogenesis from leaf and petiole explants reported here will be of great use for the rapid and large scale clonal propagation, production of biomass for extraction of cardiotonic glycosides, ex situ conservation, and improvement through conventional plant breeding and transgenic methods.

1 aBhusare, Bhushan1 aJohn, C., K.1 aBhatt, V., P.1 aNikam, T., D. uhttps://library.ncl.res.in/content/vitro-propagation-digitalis-lanata-ehrh-through-direct-shoot-regeneration-source-cardiotonic00641nas a2200169 4500008004100000022001400041245011900055210006900174260000800243300001200251490000800263100002000271700001700291700001800308700001800326856012700344 2020 eng d a0254-629900aInduction of somatic embryogenesis in leaf and root explants of Digitalis lanata Ehrh.: direct and indirect method0 aInduction of somatic embryogenesis in leaf and root explants of  cMAY a356-3650 v1301 aBhusare, B., P.1 aJohn, C., K.1 aBhatt, V., P.1 aNikam, T., D. uhttps://library.ncl.res.in/content/induction-somatic-embryogenesis-leaf-and-root-explants-digitalis-lanata-ehrh-direct-and01513nas a2200241 4500008004100000022001400041245013700055210006900192260000800261300001100269490000800280520067900288653002300967653003400990653002101024653001401045653001201059100002001071700001701091700001801108700001801126856012701144 2021 eng d a0926-669000aColchicine induces tetraploids in in vitro cultures of digitalis lanata Ehrh.: enhanced production of biomass and cardiac glycosides0 aColchicine induces tetraploids in in vitro cultures of digitalis cDEC a1141670 v1743 aThis study describes a promising method for tetraploidy induction in the in vitro grown diploid shoots of the cardiotonic herb Digitalis lanata by colchicine treatment to obtain a higher content of the cardiac glycosides' digoxin and digitoxin. The best frequency of tetraploids (33 %) induction was observed with 0.5 % colchicine treatment for 8 h. The content of digoxin and digitoxin in the tetraploids increased by 1.61-fold and 1.73-fold over the control diploids respectively. The higher cardiotonic glycoside production in the tetraploid plants of D. lanata have the potential to satisfy the increasing demand of superior plant material for pharmaceutical industries.10aCardiac glycosides10aColchicine induced polyploidy10aDigitalis lanata10aDigitoxin10aDigoxin1 aBhusare, B., P.1 aJohn, C., K.1 aBhatt, V., P.1 aNikam, T., D. uhttps://library.ncl.res.in/content/colchicine-induces-tetraploids-vitro-cultures-digitalis-lanata-ehrh-enhanced-production03923nas a2200169 4500008004100000245010000041210006900141260000800210300001000218490000700228520332200235100002003557700001803577700001703595700001803612856012303630 2021 eng d00aUraria picta: a comprehensive review on evidences of utilization and strategies of conservation0 aUraria picta a comprehensive review on evidences of utilization  cAPR a41-480 v133 a

Uraria picta (Jacq.) DC. (Prishnaparni) is one of the most important medicinal plants used in different traditional systems of medicines including the Ayurveda and Traditional Chinese medicine. The major use of this plant was found in the most popular Ayurvedic formulation “Dashmula” and in several many other important Ayurvedic formulations. IUCN placed this woody herb in the least concern category as per version 3.1. It has extensive therapeutic uses and pharmacological activities. Though this plant is a source of many phytochemicals, the uses are uncertain because the raw plant parts or crude extracts are being used in all formulations. Therefore, extensive investigations are necessary to focus on the identification of these phytochemicals. It is an urgent need to give special attention to collecting various aspects and more efforts are required in all areas for utilization and conservation of this valuable medicinal herb. Herein, a compilation of all information with various aspects has been presented, including the authors published work on Uraria picta. This review pursues attention towards biological activity, phytochemical profile, utilization, propagation and conservation of Uraria picta.

1 aBhusare, B., P.1 aAhire, M., L.1 aJohn, C., K.1 aNikam, T., D. uhttps://library.ncl.res.in/content/uraria-picta-comprehensive-review-evidences-utilization-and-strategies-conservation